LOCUS       sequence-26524-e         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Bunnell SC, Diehn M, Yaffe MB, Findell PR, Cantley LC, Berg LJ
  TITLE     Biochemical interactions integrating Itk with the T cell 
            receptor-initiated signaling cascade.
  JOURNAL   J Biol Chem. 2000 Jan 21. 275(3):2219-30.
  PUBMED    10636929
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Jan 21. 275(3):2219-30."
COMMENT     Sequence Label: pGEX Itk TEC
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(321..343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(488..506)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        574..678
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctggttccgc gtggatccga gagtcggcag cgctgggtgc tgacccttaa agaagaaacg
       61 aggaataaca acagcctggt atccaagtat caccctaatt tctggatgga tgggcggtgg
      121 aggtgctgct cccagctgga gaagcctgct gtaggctgtg ctccctacga cccatccaag
      181 aatgcttcaa agaagcctct tcctcctact cctgaagaca acaggcggtc atttcaggaa
      241 cctgaagaaa ccggatcccc gggaattcat cgtgactgac tgacgatctg cctcgcgcgt
      301 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
      361 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
      421 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga
      481 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      541 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
      601 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
      661 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
      721 gcggcatttt gccttcctgt ttttgctcac ccagaaac
//
