LOCUS       sequence-265248-        3617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression clone from T7 promoter for full-length (signal peptide 
            containing) B. burgdorferi BB0238 with C-terminal 
            TEV-protease-cleavable His12.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Robertson KE, Truong CD, Craciunescu FM, Chiu PL, Fromme P, Hansen 
            DT
  TITLE     Membrane directed expression in Escherichia coli of BBA57 and other 
            virulence factors from the Lyme disease agent Borrelia burgdorferi.
  JOURNAL   Sci Rep. 2019 Nov 26;9(1):17606. doi: 10.1038/s41598-019-53830-x.
  PUBMED    31772280
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-11-26"; volume: "9(1):17606. doi"; pages: " 
            10.1038/s41598-019-53830-x"
COMMENT     Sequence Label: pBB0238-TEV-His12
FEATURES             Location/Qualifiers
     source          1..3617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             868..888
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             889..918
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             892..921
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             895..924
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     primer_bind     complement(1018..1036)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1032..1079
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1176..1631
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1263..1282)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1473..1494
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1657..1761
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1762..1830,1831..2622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1980..1999)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2793..3381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3282..3301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3535..3552
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcgtcgtct gttcctgctg
      121 tatatcctgt gcagctttgt tttcctgaac ctgtttgcgc aaggtagcag cagctacatt
      181 gacaaacaga aggagctggc gatcttctac tatgaagtgg gccaacgtta catcaacgtt
      241 ggcaagatca aaaagggcaa gctgtttcag gcgaaggcgc tgaaaattta cccggatctg
      301 aaaaagggtt tcgacatcaa actggcggtg aaggagctgg atgcgcgtat taaagacgat
      361 aacccgaagg ttgtgatgct ggaagacatc aagctggagg aaattccggg catcgttcac
      421 gagaagatcg aaatcaacga tttcaccaac gcgccgaaga ttgagtacat cgcgcagcgt
      481 gaacgtagca agaaccaaga caagatcatc aagttccagt tcggcaagtt cgcgcgtgcg
      541 ctgattagcc gtaactttga cctgttcgac agcgtgatcg cggataaggt gaacgttatg
      601 ggccaatttg aaagcaaaaa cgacttcatc agcaccctga gcagcgcgag cagcaaagcg
      661 gatgcggacg agctggaata cctgagcgtg gacgattact atgatctgaa gagcctgaaa
      721 atcagcaaga gcaacgacac cagcttcgcg gtgaacgtta acgcgaaaaa gaacgacgtt
      781 accaaaaact ttccgttctg gaaggagcgt cagaccctga tttttaccac cgaagacgat
      841 aacaactggt tcctgagcag catcaacgaa aatctttatt ttcaaggtca ccatcatcac
      901 caccatcacc atcatcacca ccattaataa aagggcgaat tccagcacac tggcggccgt
      961 tactagtgga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     1021 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     1081 tgaaaggagg aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc
     1141 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     1201 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     1261 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     1321 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     1381 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     1441 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     1501 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     1561 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     1621 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     1681 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     1741 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     1801 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1861 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1921 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1981 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2041 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2101 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2161 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2221 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2281 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2341 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2401 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2461 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2521 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     2581 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     2641 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     2701 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     2761 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     2821 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2881 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     2941 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3001 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3061 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3121 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3181 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3241 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3301 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3361 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3421 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3481 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3541 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     3601 ccgattcatt aatgcag
//
