LOCUS       sequence-265260-        7698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-PCAF fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7698)
  AUTHORS   Rafalska-Metcalf IU, Powers SL, Joo LM, LeRoy G, Janicki SM
  TITLE     Single cell analysis of transcriptional activation dynamics.
  JOURNAL   PLoS One. 2010 Apr 21;5(4):e10272. doi: 
            10.1371/journal.pone.0010272.
  PUBMED    20422051
REFERENCE   2  (bases 1 to 7698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-21"; volume: "5(4):e10272. doi"; pages: " 
            10.1371/journal.pone.0010272"
COMMENT     Sequence Label: YFP-PCAF-C2
FEATURES             Location/Qualifiers
     source          1..7698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(25..27,28..30,31..741)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(70..91)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(331..350)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     678..699
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(776..792)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(777..793)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    4138..4259
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4175..4194)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4229..4248
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4266..4721)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4403..4424)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4615..4634
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4748..4852
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4854..5211
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4854..5045
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      5062..5197
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5246..6040
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5246..6040
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5300..5319)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5910..5929
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6228..6247)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6272..6319
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6648..7236
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7137..7156
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7369..7698
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7549..7684
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 aagctagcgc taccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
       61 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      121 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      181 aagctgcccg tgccctggcc caccctcgtg accaccttcg gctacggcct gcagtgcttc
      241 gcccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      301 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      361 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      421 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      481 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      541 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      601 cccgtgctgc tgcccgacaa ccactacctg agctaccagt ccgccctgag caaagacccc
      661 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      721 ggcatggacg agctgtacaa gtccggccgg actcagatct cgagctcaag cttatcgata
      781 ccgtcgacct cgaggaagga agacgcgtcc ggtcggggcg gcatggccga ggctggcggg
      841 gccgggtccc cagcactgcc tcccgcgccc ccgcacggtt ccccccggac cctggccacc
      901 gctgccggga gctctgcttc ctgcgggcca gcgaccgcgg tggccgcggc gggcacggcc
      961 gagggaccgg gaggaggcgg ctcggcccgg atcgccgtga agaaggcgca gttgcgctct
     1021 gctccgcggg cgaagaagct ggagaaactc ggcgtgtact ccgcctgcaa ggcagaggag
     1081 tcctgtaaat gcaatggctg gaagaaccct aacccctctc ctactccacc aagaggagac
     1141 ctccagcaga taattgtcag tttgacagaa tcctgtcgaa gctgtagcca tgcccttgct
     1201 gctcacgttt ctcacttgga gaatgtgtca gaggaagaga tggacagact cctgggaatt
     1261 gtgttggatg tggagtacct cttcacctgc gtccacaaag aagaagatgc agataccaaa
     1321 caagtgtact tctacctatt caagctcttg agaaagtcaa ttttacaaag aggaaaacct
     1381 gtggttgaag gctccttgga gaagaagccg ccatttgaga agcccagtat tgaacagggt
     1441 gtgaacaact tcgtgcagta caagtttagt cacttgccat cgaaagagag gcagacaacg
     1501 atcgagctgg ccaagatgtt tctgaaccgc atcaactact ggcatctgga ggctccatct
     1561 cagcggagac tacggtctcc caatgatgac atctctggat acaaggaaaa ctacacaagg
     1621 tggttgtgct actgcaatgt accgcagttc tgtgacagct tacctcggta cgaaaccaca
     1681 aaggtgtttg ggagaacatt gcttcgctcg gtcttcacca tcatgagacg acagctcttg
     1741 gaacaagcca gacaggaaaa agacaaactg cctcttgaga aacgcacgct tatcctcaca
     1801 catttcccaa agtttctgtc catgttggaa gaagaagtgt atagtcaaaa ttctcctatc
     1861 tgggatcagg attttctctc agcctcttcc agaaccagcc cgctaggaat ccaaacagta
     1921 atcagtcctc ctgttactgg gacagcattg ttcagttcaa attccacttc ccatgagcag
     1981 atcaatggag ggagaacaag tcctggatgc agaggctctt ctgggcttga agcaaacccg
     2041 ggagaaaaga ggaaaatgaa caactctcat gctcccgagg aggccaagag atctcgagtg
     2101 atgggggata ttcccgtgga attgatcaat gaggtcatgt ctaccatcac agaccctgca
     2161 gggatgcttg gaccagagac caattttctg tcagcccatt cggccagaga tgaggcggca
     2221 cggctggaag aacgcagggg tgtcattgaa ttccacgtgg tgggcaactc cctgaaccag
     2281 aaaccaaaca agaagatcct gatgtggctc gtgggcctcc agaatgtgtt ttcccaccag
     2341 ctgcccagaa tgcccaaaga gtacatcaca cggctcgtct ttgacccgaa acacaaaacc
     2401 cttgctttaa ttaaagatgg ccgtgtcatt ggtggtatct gtttccggat gtttccatcc
     2461 cagggattca cagagattgt tttctgtgca gtaacctcaa atgaacaagt caagggctat
     2521 ggaacccacc tgatgaacca tctcaaagaa taccacataa agcacgagat cctcaacttc
     2581 ctcacatatg cagatgagta tgccatcggc tatttcaaga agcagggttt ctccaaagaa
     2641 atcaaaatac ctaaaaccaa atatgttggc tacatcaagg attatgaagg ggccactttg
     2701 atgggatgtg agctgaaccc tcagatccca tacacagagt tctctgtcat cattaaaaag
     2761 cagaaggaga tcattaaaaa gctgatagaa agaaaacaag cccagattcg aaaagtctac
     2821 cctggacttt cgtgtttcaa agatggagtt cggcagattc ctatagaaag cattcctgga
     2881 atcagagaga caggctggaa accaagtgga aaagagaaaa gtaaagagcc caaagaccct
     2941 gagcagcttt acagcaccct caagaacatc ctgcagcagg tgaagaacca tccaaatgct
     3001 tggcctttca tggaaccagt gaagagaaca gaagctccgg gatattatga agttataagg
     3061 ttccccatgg atctgaaaac catgagtgaa cgcctcagga acaggtacta tgtgtctaag
     3121 aagttattca tggcggactt gcaacgagtg ttcaccaact gcaaggagta caaccctccc
     3181 gagagcgagt actacaaatg cgccagcatc ctggagaagt tcttcttcag taaaattaag
     3241 gaagcagggt tgattgacaa gtgatttctt tcccttagac acacatcagc agtgtgccta
     3301 aaacaggtca tttaggtttt tggttgcttt tgtttttttg tttttgtttt taaattgtac
     3361 atcatgtgtt gaagagactt gtaataatta gcacttttga aaaacaaaca aaacctcctt
     3421 ttagcttttc agagacgtat ttaaatggaa atcatagatt ttttttttat tttgagcaat
     3481 agattttaat ctatttacta ctatcatgta atttttctct ggcatgtcca gtagctgact
     3541 attgcatggt agatgatcag gggcttcctc aaaacctgta tgtgaggaaa ttgcacacag
     3601 taccaagatt tggggtaatc tagagaaaag tttgaccatg aatgtctcca ttttttttct
     3661 aatggaacat gagagtttat tttattctga aggactttaa agggtccgtg acacacctgt
     3721 gctgtgaatg aactgtgtga tgtttgaagt ctccttgtag actttttata agtatatatt
     3781 tttaaaacac tcatctagat gaggtgcttt gagcagttct gaaaaaaaaa aatgcagctt
     3841 ccacaatagc ggctgctatg actcctaagg aagggacttt ttataagctc ctggggcttt
     3901 gatcattctg tctacctatt acagacttga gagtgcacaa ccactattta ataatatctt
     3961 ggcagatatg tgtccatctc atatttgact ttgcttgtgc agggcccggg atccaccgga
     4021 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     4081 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     4141 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     4201 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     4261 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     4321 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     4381 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4441 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4501 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4561 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4621 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4681 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     4861 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4921 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4981 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     5041 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     5101 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     5161 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     5221 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     5281 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     5341 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     5401 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     5461 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     5521 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     5581 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     5641 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     5701 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     5761 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     5821 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     5881 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     5941 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     6001 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     6061 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     6121 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     6181 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     6241 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     6301 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     6361 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     6421 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     6481 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     6541 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6601 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6661 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6721 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6781 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6841 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6901 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6961 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     7021 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7081 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7141 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7201 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7261 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7321 tctgtggata accgtattac cgccatgcat tagttattac tgtggaatgt gtgtcagtta
     7381 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7441 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7501 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7561 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7621 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7681 ggcctaggct tttgcaaa
//
