LOCUS       sequence-265264-       12568 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion and 
            Psd95 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12568)
  AUTHORS   Schluter OM, Xu W, Malenka RC
  TITLE     Alternative N-terminal domains of PSD-95 and SAP97 govern 
            activity-dependent regulation of synaptic AMPA receptor function.
  JOURNAL   Neuron. 2006 Jul 6;51(1):99-111.
  PUBMED    16815335
REFERENCE   2  (bases 1 to 12568)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2006-07-6"; volume: "51(1)"; pages: "99-111"
COMMENT     Sequence Label: FH95pUp95bGW (B5)
FEATURES             Location/Qualifiers
     source          1..12568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2373..2587
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2508..2527
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2691..3902
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3739..3758
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      6261..6270
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6267..6269,6270..6272,6273..6986)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6267..6269,6270..6272,6273..6983)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6312..6333)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6573..6592)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6920..6941
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7029..7617
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7082..7102)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7500..7511)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8136..8316
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8342..8359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8348..8572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8618..9046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8705..8724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8915..8936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9055..9075)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9060..9389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9240..9375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9302..9321
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9437..9484
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9503..9877
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10007..10128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10044..10063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10098..10117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10177..10193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10177..10193)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10190..10212)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10201..10217
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10225..10231,10232..10249,10250..10255))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10270..10291
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10408..10425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10579..11167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10659..10678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11338..12129,12130..12198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11961..11980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12199..12303)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12378..12397)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttaa tcgtacgaat tcgaacgctg acgtcatcaa cccgctccaa
     2401 ggaatcgcgg gcccagtgtc actaggcggg aacacccagc gcgcgtgcgc cctggcagga
     2461 agatggctgt gagggacagg ggagtggcgc cctgcaatat ttgcatgtcg ctatgtgttc
     2521 tgggaaatca ccataaacgt gaaatgtctt tggatttggg aatcttataa gttctgtatg
     2581 agaccacaga tccccggaca tccaggcaca caagttcaag agacttgtgt gcctggatgt
     2641 cctttttgga aagcttatcg aattaattaa cccgtgtcgg ctccagatct ggcctccgcg
     2701 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2761 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2821 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2881 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2941 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     3001 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     3061 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     3121 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     3181 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     3241 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     3301 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     3361 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     3421 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     3481 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3541 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3601 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3661 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3721 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3781 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3841 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3901 acgaagcttg ggctgcaggt cgactctaga ccaccatgtc ccagagacca agagctccca
     3961 ggtcagccct ctggctcctg gccccgccac tattgcggtg ggcacctccc ctcctcacag
     4021 tgctgcacag tgacctcttc caggccttgc tggacatcct ggactattat gaggcttgca
     4081 tcttagagag tcagaaatac cgctaccaag atgaagacac gccccctctg gaacacagcc
     4141 cggcccacct ccccaaccag gccaattctc cccctgtgat tgtcaacacg gacaccctag
     4201 aagccccagg atatgagttg caggtgaatg gaacagaggg ggagatggag tatgaggaga
     4261 tcacattgga aaggggtaac tcaggtctgg gcttcagcat cgcaggtggc actgacaacc
     4321 cgcacatcgg tgacgacccg tccattttta tcaccaagat cattcctggt ggggctgcag
     4381 cccaggatgg ccgcctcagg gtcaatgaca gcatcctgtt tgtaaatgaa gtggatgttc
     4441 gggaggtgac ccattcagct gcggtggagg ccctcaaaga ggcaggttcc atcgttcgcc
     4501 tctatgtcat gcgccggaaa cccccagccg aaaaggtcat ggagatcaaa ctcatcaaag
     4561 ggcctaaagg acttggcttc agcattgcgg ggggcgttgg gaaccagcac atccctggag
     4621 ataacagcat ctatgtaacg aagatcatcg aaggaggtgc tgcccacaag gatggcaggt
     4681 tgcagattgg agacaagatc ctggcggtca acagtgtggg gctggaggac gtcatgcacg
     4741 aggatgccgt ggcagccctg aagaacacat atgacgttgt gtacctaaag gtggccaagc
     4801 ccagcaatgc ctacctgagt gacagctatg ctcccccaga catcacaacc tcgtattctc
     4861 agcacctgga caatgagatc agtcatagca gctacttggg cactgactac cccacagcca
     4921 tgacccccac ttcccctcgg cgctactccc ctgtggccaa ggacctgctg ggggaggaag
     4981 acattccccg ggaaccaagg cggatcgtga tccatcgggg ctccaccggc ctgggcttca
     5041 acatcgtggg cggcgaggat ggtgaaggca tcttcatctc cttcatcctt gctgggggtc
     5101 cagccgacct cagtggggag ctacggaagg gggaccagat cctgtcggtc aatggtgttg
     5161 acctccgcaa tgccagtcac gaacaggctg ccattgccct gaagaatgcg ggtcagacgg
     5221 tcacgatcat cgctcagtat aaaccagaag agtatagtcg attcgaggcc aagatccatg
     5281 atcttcggga acagctcatg aatagtagcc taggctcagg gactgcatcc ttgcgaagca
     5341 accccaagag gggcttctac attagggccc tgtttgatta cgacaagacc aaggactgcg
     5401 gtttcttgag ccaggccctg agcttccgct tcggggatgt gcttcatgtc attgacgctg
     5461 gtgacgaaga gtggtggcaa gcacggcggg tccactccga cagtgagacc gacgacattg
     5521 gcttcattcc cagcaaacgg cgggtcgagc gacgagagtg gtcaaggtta aaggccaagg
     5581 actggggctc cagctctgga tcacagggtc gagaagactc ggttctgagc tatgagacgg
     5641 tgacccagat ggaagtgcac tatgctcgtc ccatcatcat ccttggaccc accaaagacc
     5701 gtgccaacga tgatcttctc tccgagttcc ccgacaagtt tggatcctgt gtccctcata
     5761 cgacacgtcc taagcgggaa tatgagatag acggccggga ttaccacttt gtctcctccc
     5821 gggagaaaat ggagaaggat attcaagcgc ataagttcat tgaggctggc cagtacaaca
     5881 gccacctcta tgggaccagc gtccagtctg tgcgagaggt agcagagcag gggaagcact
     5941 gcatcctcga tgtctcggcc aatgccgtgc ggcggctgca ggcggcccac ctgcacccca
     6001 tcgccatctt catccgtccc cgctccctgg agaatgtgct agagatcaat aagcggatca
     6061 cagaggagca agcccggaaa gccttcgaca gagccacgaa gctggagcag gagttcacag
     6121 agtgcttctc agccatcgta gagggcgaca gctttgaaga gatctatcac aaagtgaaac
     6181 gtgtcattga agacctctca ggcccctaca tctgggtccc agcccgagag agactcggta
     6241 ccgcgggccc gggatccatc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     6301 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6361 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6421 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     6481 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6541 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6601 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     6661 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     6721 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     6781 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     6841 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     6901 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     6961 tcggcatgga cgagctgtac aagtaagcta gctcgagcta gaattaattc gatatcaagc
     7021 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     7081 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7141 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7201 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7261 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7321 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7381 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     7441 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7501 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7561 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     7621 gataccgtcg agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca
     7681 atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac
     7741 ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7801 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc
     7861 tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag
     7921 ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga
     7981 aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg
     8041 ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc
     8101 atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct
     8161 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     8221 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     8281 tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca
     8341 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     8401 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     8461 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     8521 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag
     8581 gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta
     8641 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8701 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8761 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8821 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8881 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8941 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9001 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
     9061 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     9121 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     9181 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     9241 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9301 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     9361 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     9421 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
     9481 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
     9541 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
     9601 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
     9661 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
     9721 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
     9781 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
     9841 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
     9901 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     9961 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
    10021 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    10081 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
    10141 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
    10201 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
    10261 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
    10321 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
    10381 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
    10441 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
    10501 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
    10561 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
    10621 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
    10681 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
    10741 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
    10801 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
    10861 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
    10921 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
    10981 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
    11041 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
    11101 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
    11161 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
    11221 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
    11281 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
    11341 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    11401 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    11461 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    11521 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    11581 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    11641 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    11701 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    11761 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    11821 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    11881 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    11941 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    12001 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    12061 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    12121 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    12181 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    12241 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    12301 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
    12361 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
    12421 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
    12481 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
    12541 cgcgatgtac gggccagata tacgcgtt
//
