LOCUS       sequence-265265-        7036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Cherry-ICP0 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM
  TITLE     Single-cell analysis of Daxx and ATRX-dependent transcriptional 
            repression.
  JOURNAL   J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 
            10.1242/jcs.110148. Epub 2012 Sep 12.
  PUBMED    22976303
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-11-15"; volume: "125(Pt 22):5489-501. doi"; 
            pages: " 10.1242/jcs.110148. Epub 2012 Sep 12"
COMMENT     Sequence Label: Cherry-ICP0-C3
FEATURES             Location/Qualifiers
     source          1..7036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..1260
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(698..716)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     966..985
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    3764..3885
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3801..3820)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3855..3874
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3892..4347)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4029..4050)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4241..4260
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4374..4478
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4480..4837
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4480..4671
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4503..4523)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4688..4823
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4872..5666
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4872..5666
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4926..4945)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5536..5555
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5854..5873)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5898..5945
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6274..6862
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6763..6782
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      601 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      661 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      721 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      781 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      841 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      901 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      961 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1021 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1081 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1141 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1201 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1261 tactcagatc tcgacatgga gccccgcccc ggagcgagta cccgccggcc tgagggccgc
     1321 ccccagcgcg agcccgcccc ggatgtctgg gtgtttccct gcgaccgaga cctgccggac
     1381 agcagcgact ctgaggcgga gaccgaagtg ggggggcggg gggacgccga ccaccatgac
     1441 gacgactccg cctccgaggc ggacagcacg gacacggaac tgttcgagac ggggctgctg
     1501 gggccgcagg gcgtggatgg gggggcggtc tcggggggga gccccccccg cgaggaagac
     1561 cccggcagtt gcgggggcgc cccccctcga gaggacgggg ggagcgacga gggcgacgtg
     1621 tgcgccgtgt gcacggatga gatcgcgccc cacctgcgct gcgacacctt cccgtgcatg
     1681 caccgcttct gcatcccgtg catgaaaacc tggatgcaat tgcgcaacac ctgcccgctg
     1741 tgcaacgcca agctggtgta cctgatagtg ggcgtgacgc ccagcgggtc gttcagcacc
     1801 atcccgatcg tgaacgaccc ccagacccgc atggaggccg aggaggccgt cagggcgggc
     1861 acggccgtgg actttatctg gacgggcaat cagcggttcg ccccgcggta cctgaccctg
     1921 ggggggcaca cggtgagggc cctgtcgccc acccacccgg agcccaccac ggacgaggat
     1981 gacgacgacc tggacgacgc agactacgta ccgcccgccc cccgccggac gccccgcgcc
     2041 cccccacgca gaggcgccgc cgcgcccccc gtgacgggcg gggcgtctca cgcagccccc
     2101 cagccggccg cggctcggac agcgcccccc tcggcgccca tcgggccaca cggcagcagt
     2161 aacaccaaca ccaccaccaa cagcagcggc ggcggcggct cccgccagtc gcgagccgcg
     2221 gcgccgcggg gggcgtctgg cccctccggg ggggttgggg ttggggttgg ggttgttgaa
     2281 gcggaggcgg ggcggccgag gggccggacg ggcccccttg tcaacagacc cgcccccctt
     2341 gcaaacaaca gagaccccat agtgatcagc gactcccccc cggcctctcc ccacaggccc
     2401 cccgcggcgc ccatgccagg ctccgccccc cgccccgggc cccccgcgtc cgcggccgcg
     2461 tcgggacccg cgcgcccccg cgcggccgtg gccccgtgcg tgcgagcgcc gcctccgggg
     2521 cccggccccc gcgccccggc ccccggggcg gagccggccg cccgccccgc ggacgcgcgc
     2581 cgtgtgcccc agtcgcactc gtccctggct caggccgcga accaagaaca gagtctgtgc
     2641 cgggcgcgtg cgacggtggc gcgcggctcg ggggggccgg gcgtggaggg tgggcacggg
     2701 ccctcccgcg gcgccgcccc ctccggcgcc gccccgctcc cctccgccgc ctctgtcgag
     2761 caggaggcgg cggtgcgtcc gaggaagagg cgcgggtcgg gccaggaaaa cccctccccc
     2821 cagtccacgc gtccccccct cgcgccggca ggggccaaga gggcggcgac gcaccccccc
     2881 tccgactcag ggccgggggg gcgcggccag ggtgggcccg ggacccccct gacgtcctcg
     2941 gcggcctccg cctcttcctc ctctgcctct tcctcctcgg ccccgacccc cgcgggggcc
     3001 gcctcttccg ccgccggggc cgcgtcctcc tccgcttccg cctcctcggg cggggccgtc
     3061 ggtgccctgg gagggagaca agaggaaacc tccctcggcc cccgcgctgc ttctgggccg
     3121 cgggggccga ggaagtgtgc ccggaagacg cgccacgcgg agacttccgg ggccgtcccc
     3181 gcgggcggcc tcacgcgcta cctgcccatc tcgggggtct ctagcgtggt cgccctgtcg
     3241 ccttacgtga acaagactat cacgggggac tgcctgccca tcctggacat ggagacgggg
     3301 aacatcgggg cgtacgtggt cctggtggac cagacgggaa acatggcgac ccggctgcgg
     3361 gccgcggtcc ccggctggag ccgccgcacc ctgctccccg agaccgcggg taaccacgtg
     3421 atgccccccg agtacccgac ggcccccgcg tcggagtgga acagcctctg gatgaccccc
     3481 gtggggaaca tgctgttcga ccagggcacc ctagtgggcg ccctggactt ccgcagcctg
     3541 cggtctcggc acccgtggtc cggggagcag ggggcgtcga cccgggacga gggaaaacaa
     3601 taagaattct gcagtcgacg gtaccgcggg cccgggatcc accggatcta gataactgat
     3661 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     3721 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     3781 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     3841 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa
     3901 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     3961 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     4021 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     4081 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     4141 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     4201 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     4261 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     4321 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
     4381 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4441 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     4501 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4561 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4621 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4681 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4741 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4801 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     4861 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     4921 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     4981 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     5041 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     5101 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     5161 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     5221 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     5281 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     5341 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     5401 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     5461 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     5521 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     5581 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     5641 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     5701 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     5761 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     5821 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     5881 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     5941 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6001 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     6061 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     6121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6541 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6601 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6661 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6721 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6781 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6841 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6901 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6961 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7021 catatatgga gttccg
//
