LOCUS       sequence-265266-        7732 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-GCN5 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Rafalska-Metcalf IU, Powers SL, Joo LM, LeRoy G, Janicki SM
  TITLE     Single cell analysis of transcriptional activation dynamics.
  JOURNAL   PLoS One. 2010 Apr 21;5(4):e10272. doi: 
            10.1371/journal.pone.0010272.
  PUBMED    20422051
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-21"; volume: "5(4):e10272. doi"; pages: " 
            10.1371/journal.pone.0010272"
COMMENT     Sequence Label: YFP-mGCN5-C1
FEATURES             Location/Qualifiers
     source          1..7732
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..965)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(294..315)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(555..574)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     902..923
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4156..4277
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4193..4212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4247..4266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4284..4739)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4421..4442)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4633..4652
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4766..4870
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4872..5229
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4872..5063
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4895..4915)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5080..5215
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5264..6058
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5264..6058
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5318..5337)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5928..5947
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6246..6265)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6290..6337
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6666..7254
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7155..7174
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        7429..7732
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg
      241 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
      301 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
      361 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      421 ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc taccccgacc
      481 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      541 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
      601 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      661 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
      721 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
      781 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      841 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      901 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
      961 acaagtccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccatgg
     1021 cggaaccttc ccaggcccca aacccggtcc cggcagcgca gccccggccc cttcactccc
     1081 cagcccctgc cccaacttcg actcctgcgc ccagtccggc ttcagcctcc actccggctc
     1141 cgactccggc accagcccct gccccagctg cagccccagc tgggagcaca gggagtgggg
     1201 gcgccggggt aggaagtggg ggggatccgg ctcgacctgg cctgagccag cagcagcgcg
     1261 ctagccagag gaaggcgcaa gtccggggtc tgccgcgtgc caagaagctt gagaaactag
     1321 gggtcttctc agcttgcaag gccaatgaaa cctgcaagtg taatggctgg aaaaacccca
     1381 agccccccac cgcaccccgc atggacctcc agcagccagc tgccaacctg agcgagttgt
     1441 gccgtagctg tgagcaccct ttggcggacc atgtgtccca cctggagaat gtgtcagagg
     1501 acgagattaa ccggctcttg ggaatggtag tagatgtaga aaatctgttc atgtctgttc
     1561 acaaggaaga ggacacagac accaaacaag tctatttcta cctcttcaag ctcttgcgga
     1621 agtgcattct gcagatgaca cggccagtgg tggaggggtc tctaggcagc cccccatttg
     1681 agaagcctaa cattgaacag ggtgtactga actttgtgca gtacaaattt agccacctgg
     1741 ccccccgaga gcgccagaca atgtttgagc tctcaaagat gttcctgctc tgccttaact
     1801 actggaagct ggagacccct gctcaattcc ggcagcggtc ccagtctgag gatgtcgcta
     1861 cctacaaagt caattatacc agatggctct gttactgcca cgtgccccag agctgcgaca
     1921 gcctcccccg ctacgagacc acccacgtgt ttggccgaag ccttctacgg tccattttca
     1981 ctgtcacccg ccggcagcta ctggaaaaat ttcgggtgga aaaggacaaa ctggtgcctg
     2041 agaagaggac cctcatcctc actcacttcc ccaaattcct gtccatgctt gaggaagaga
     2101 tctacggggc aaattctccc atctgggagt caggcttcac catgccacct tcggagggga
     2161 cacagctggt gccccggcca gccacggtca gtgcaacagt tgtacccagc ttcagtcctt
     2221 ccatgggagg aggcagcaac agctccctga gcctagattc cgcagggact gagcccatgc
     2281 cagcagggga gaagaggaag cttcctgaga acctgaccct ggaggatgcc aagcggctcc
     2341 gcgtgatggg ggacatcccc atggagctgg tcaatgaggt catgctgacg atcactgacc
     2401 ccgctgccat gctggggcct gagacaagcc tgctttcggc caacgcagct cgagatgaga
     2461 cggctcgcct ggaagaacgg cgaggaatca tcgagttcca cgtcattggc aactccctga
     2521 cgcccaaggc caaccggcgc gtgctgctct ggctcgtggg gctgcagaat gtcttttccc
     2581 accagctgcc aagaatgccc aaggaatata tcgcccgcct cgtctttgac ccgaagcaca
     2641 agaccctggc cctgatcaag gatgggcggg tcatcggtgg catttgcttc cgcatgtttc
     2701 ccactcaggg cttcacggaa atcgtcttct gtgccgtcac ctcaaatgag caggtcaagg
     2761 gctatggcac ccacctgatg aaccacctaa aggagtacca catcaaacac agcatccttt
     2821 actttctcac ctatgctgac gagtacgcca ttggctactt caaaaagcag ggcttctcca
     2881 aagacatcaa ggtgcccaag agccgctatc tgggctacat caaggactat gaaggagcga
     2941 cgctgatgga gtgtgagctg aatccccgaa tcccctacac agagctgtcc cacatcatca
     3001 agaaacagaa agagatcatc aagaagttga ttgagcgcaa acaggcacag atccgcaagg
     3061 tctaccctgg actcagctgc ttcaaagagg gagtgaggca gatcccggtg gagagcgtcc
     3121 ctggcattcg agagacaggc tggaagccac tgggaaagga gaagggcaag gagcttaagg
     3181 atcctgatca gctatatacg accctcaaaa acctgctggc tcagatcaag tcacacccca
     3241 gtgcctggcc cttcatggaa cctgtgaaga agtcagaggc tcctgactac tacgaggtta
     3301 tccgtttccc cattgacctg aagaccatga cagaacggtt gcgcagtcgc tactatgtga
     3361 cccggaagct cttcgtagct gacctgcagc gggtcatcgc caactgccgg gagtacaatc
     3421 ccccagacag cgaatactgt cgctgcgcca gtgccctgga gaagttcttc tacttcaagc
     3481 tcaaagaggg agggctcatc gacaagtagc ccccagctgg gatccgcaac atccacctcc
     3541 atctcctcag atccgtttgg gggctcccgg ccccctctcc cggactcagc tggagaaact
     3601 ccagccaggg accccagatc ttacagactt tctggccagc aggcactcac tcccaagcca
     3661 gcagctctct ttccaccttc cttgcatccc accttgggga gacgtctggt ctggggccag
     3721 cccgttgtag aatgtctggt ggccagggca tgaactgttg gtccttatag agccatgcag
     3781 gctgagcatc ctgagcctgg tccccatgcc tgtttcccag ggccaggact attcagtgag
     3841 tagccttcac atggaaacag cccagactgg aggaaccatg aggggccttg gcttatccta
     3901 cactgttgtc tatctgtccc tccaggattg ggggcaggag gaccattcat tccttggcat
     3961 taatccctcg gagggaacaa taaagctttt tttttcccct gaaaaaaaaa aaaaagcttg
     4021 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
     4081 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     4141 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     4201 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     4261 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     4321 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     4381 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     4441 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     4501 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     4561 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     4621 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     4681 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     4741 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     4801 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     4861 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     4921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4981 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     5101 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     5161 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     5221 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     5281 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     5341 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     5401 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     5461 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     5521 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     5581 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     5641 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     5701 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     5761 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     5821 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     5881 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     5941 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6001 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     6061 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     6121 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     6181 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     6241 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     6301 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     6361 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     6421 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     6481 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     6541 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     6601 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     6661 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     6721 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6781 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6841 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6901 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6961 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7021 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     7081 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     7141 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     7201 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     7261 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     7321 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     7381 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     7441 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     7501 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     7561 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     7621 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     7681 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tg
//
