LOCUS       sequence-265275-        4032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC37A3
FEATURES             Location/Qualifiers
     source          1..4032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2157..2256)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2273..2292)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2274..2292)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2297..2313)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2297..2313)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2415..2434)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2426..3235
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2493..2512)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3382..3970
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3871..3890
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatggcct ggcccaacgt gttccagagg ggctctctgc tgagccagtt
      721 ttcccaccac cacgtggtgg tgttcctgct gaccttcttt agctattccc tgctgcacgc
      781 ctcccgcaag acattttcta atgtgaaggt gtctatcagc gagcagtgga ccccaagcgc
      841 cttcaacaca tccgtggagc tgcccgtgga gatctggagc tccaatcacc tgtttcctag
      901 cgccgagaag gccaccctgt tcctgggcac cctggacaca atctttctgt tctcttacgc
      961 cgtgggcctg tttatcagcg gcatcgtggg cgataggctg aacctgcgct gggtgctgtc
     1021 cttcggcatg tgctctagcg ccctggtggt gttcgtgttc ggcgccctga ccgagtggct
     1081 gcggttctat aacaagtggc tgtactgctg tctgtggatc gtgaatggcc tgctgcagag
     1141 cacaggatgg ccatgcgtgg tggccgtgat gggaaactgg tttggcaagg caggaagagg
     1201 agtggtgttc ggactgtggt ctgcctgcgc aagcgtgggc aatatcctgg gcgcctgtct
     1261 ggcctcctct gtgctgcagt acggctatga gtacgccttt ctggtgacag catccgtgca
     1321 gttcgcagga ggaatcgtga tcttctttgg cctgctggtg tctcccgagg agatcggcct
     1381 gagcggcatc gaggccgagg agaactttga ggaggactct cacaggcctc tgatcaacgg
     1441 cggcgagaat gaggatgagt atgagccaaa ttactctatc caggacgata gctccgtggc
     1501 ccaggtgaag gccatcagct tctaccaggc atgctgtctg ccaggcgtga tcccttattc
     1561 cctggcctac gcctgcctga agctggtgaa ctatagcttc tttttctggc tgccttttta
     1621 cctgtccaac aatttcggct ggaaggaggc cgaggccgac aagctgtcta tctggtatga
     1681 tgtgggagga atcatcggag gcaccctgca gggctttatc agcgacgtgc tgcagaagag
     1741 ggcacccgtg ctggccctgt ccctgctgct ggccgtgggc tctctgatcg gctactccag
     1801 atctcctaac gataagtcca tcaatgccct gctgatgacc gtgacaggct ttttcatcgg
     1861 cggcccatcc aatatgatct ctagcgccat ctctgccgac ctgggccggc aggagctgat
     1921 ccagagatcc tctgaggccc tggccaccgt gacaggcatc gtggacggaa gcggatccat
     1981 cggagcagca gtgggacagt atctggtgag cctgatccgg gataagctgg gctggatgtg
     2041 ggtgttctac tttttcatcc tgatgaccag ctgtacaatc gtgtttatct cccccctgat
     2101 cgtgagggag atcttctccc tggtgctgcg gagacaggcc cacatcctgc gcgaggaccc
     2161 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     2221 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2281 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2341 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     2401 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2461 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2521 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     2581 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2641 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2701 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2761 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2821 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2881 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2941 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     3001 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     3061 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     3121 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     3181 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     3241 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     3301 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3361 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3421 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3481 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3541 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3601 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3661 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3721 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3781 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3841 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3901 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3961 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     4021 tgctcacatg tt
//
