LOCUS       sequence-265281-        4038 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC2A3
FEATURES             Location/Qualifiers
     source          1..4038
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2163..2262)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2279..2298)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2280..2298)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2303..2319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2303..2319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2421..2440)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2432..3241
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2499..2518)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3388..3976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3877..3896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggca cacagaaggt gaccccagcc ctgatctttg ccatcacagt
      721 ggccaccatc ggcagcttcc agtttggcta caacaccggc gtgatcaatg cccccgagaa
      781 gatcatcaag gagttcatca ataagacact gaccgacaag ggaaacgcac cacctagcga
      841 ggtgctgctg acatccctgt ggtctctgag cgtggccatc ttttccgtgg gcggcatgat
      901 cggctccttc tctgtgggcc tgtttgtgaa ccggttcggc cggagaaatt ctatgctgat
      961 cgtgaacctg ctggcagtga ccggaggatg cttcatgggc ctgtgcaagg tggccaagtc
     1021 tgtggagatg ctgatcctgg gccggctggt catcggcctg ttttgcggcc tgtgcacagg
     1081 cttcgtgcct atgtatatcg gagagatcag cccaaccgcc ctgaggggag cctttggcac
     1141 actgaatcag ctgggcatcg tggtgggcat cctggtggcc cagatctttg gcctggagtt
     1201 catcctggga agcgaggagc tgtggccact gctgctgggc ttcaccatcc tgcctgcaat
     1261 cctgcagtcc gccgccctgc ctttttgccc agagtctccc cggttcctgc tgatcaatag
     1321 aaaggaggag gagaacgcca agcagatcct gcagcggctg tggggaaccc aggacgtgtc
     1381 ccaggatatc caggagatga aggacgagag cgccagaatg tcccaggaga agcaggtgac
     1441 agtgctggag ctgtttaggg tgagctccta ccgccagccc atcatcatct ctatcgtgct
     1501 gcagctgtct cagcagctga gcggcatcaa tgccgtgttt tactatagca ccggcatctt
     1561 caaggatgcc ggcgtgcagg agcctatcta tgccacaatc ggcgccggcg tggtgaacac
     1621 aatctttacc gtggtgtccc tgttcctggt ggagagggca ggcaggcgca ccctgcacat
     1681 gatcggactg ggaggcatgg ccttctgctc tacactgatg accgtgagcc tgctgctgaa
     1741 ggacaactac aatggcatga gcttcgtgtg catcggcgcc atcctggtgt tcgtggcctt
     1801 ctttgagatc ggcccaggcc ccatcccttg gtttatcgtg gccgagctgt tcagccaggg
     1861 accaaggcca gcagcaatgg cagtggccgg ctgttccaac tggacctcta attttctggt
     1921 gggactgctg ttcccttccg ccgcacacta cctgggagca tacgtgttca tcatctttac
     1981 aggcttcctg atcacctttc tggccttcac attctttaag gtgccagaga cacggggcag
     2041 aacctttgag gatatcacca gggccttcga gggacaggca cacggagcag accgcagcgg
     2101 caaggatggc gtgatggaga tgaattccat cgagcccgcc aaggagacca caaccaacgt
     2161 ggacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     2221 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     2281 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     2341 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     2401 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     2461 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     2521 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     2581 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     2641 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     2701 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     2761 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     2821 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     2881 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     2941 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3001 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     3061 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     3121 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     3181 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     3241 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     3301 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     3361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     3421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     3481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     3541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     3601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     3661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     3721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     3781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     3841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     3901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     3961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4021 gccttttgct cacatgtt
//
