LOCUS       sequence-26550-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Alive subunit for creating monovalent streptavidin with a single 
            femtomolar biotin binding site. Can also be used to create wild-type
            (tetravalent) streptavidin. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Howarth M, Chinnapen DJ, Gerrow K, Dorrestein PC, Grandy MR, 
            Kelleher NL, El-Husseini A, Ting AY
  TITLE     A monovalent streptavidin with a single femtomolar biotin binding 
            site.
  JOURNAL   Nat Methods. 2006 Apr . 3(4):267-73.
  PUBMED    16554831
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2006 Apr . 3(4):267-73."
COMMENT     Sequence Label: pET21a-Streptavidin-Alive
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..43
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             435..452
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             480..497
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(550..568)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      564..611
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(735..754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tnggaattcc ctctgaatat tttgtttaac tttaagaagg agatatacat atggctgaag
       61 ctggtatcac cggcacctgg tacaaccagc tgggatccac cttcatcgtt accgctggtg
      121 ctgacggtgc tctgaccggt acctacgaat ccgctgttgg taacgctgaa tctagatacg
      181 ttctgaccgg tcgttacgac tccgctccgg ctaccgacgg ttccggaacc gctctgggtt
      241 ggaccgttgc ttggaaaaac aactaccgta acgctcactc cgctaccacc tggtctggcc
      301 agtacgttgg tggtgctgaa gctcgtatca acacccagtg gttgttgacc tccggcacca
      361 ccgaagccaa cgcgtggaaa tccaccctgg ttggtcacga caccttcacc aaagttaaac
      421 cgtccgctgc ttcccatcac catcaccacc attaataaaa gcttgcggcc gcactcgagc
      481 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      541 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      601 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      661 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      721 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      781 tccccgtcaa gctctaatcg ggggctccct ttagggttcn aattagtgct tacgg
//
