LOCUS       sequence-26561-e         692 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  TITLE     Reya 2009
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MSCV-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..574
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(184..203)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     413..430
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(635..651)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(667..689)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 gctcctcgcc cttgctcacc atggtattat cgtgtttttc aaaggaaaac cacgtccccg
       61 tggttcgggg ggcctagacg tttttttaac ctcgactaaa cacatgtaaa gcatgtgcac
      121 cgaggcccca gatcagatcc catacaatgg ggtaccttct gggcatcctt cagccccttg
      181 ttgaatacgc ttgaggagag ccatttgact ctttccacaa ctatccaact cacaacgtgg
      241 cactggggtt gtgccgcctt tgcaggtgta tcttatacac gtggcttttg gccgcagagg
      301 cacctgtcgc caggtggggg gttccgctgc ctgcaaaggg tcgctacaga cgttgtttgt
      361 cttcaagaag cttccagagg aactgcttcc ttcacgacat tcaacagacc ttgcattcct
      421 ttggcgagag gggaaagacc cctaggaatg ctcgtcaaga agacagggcc aggtttccgg
      481 gccctcacat tgccaaaaga cggcaatatg gtggaaaata acatatagac aaacgcacac
      541 cggccttatt ccaagcggct tcggccagta acgttagggg gggggcggaa ttgatcccgc
      601 tcgaggttaa cgaattccgg cgcctagaga aggagtgagg gctggataaa gggaggatcg
      661 aggcggggtc gaacgaggag gttcaagggg ga
//
