LOCUS       sequence-26565-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Mejat A, Decostre V, Li J, Renou L, Kesari A, Hantai D, Stewart CL, 
            Xiao X, Hoffman E, Bonne G, Misteli T
  TITLE     Lamin A/C-mediated neuromuscular junction defects in Emery-Dreifuss 
            muscular dystrophy.
  JOURNAL   J Cell Biol. 2009 Jan 12. 184(1):31-44.
  PUBMED    19124654
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2009 Jan 12. 184(1):31-44."
COMMENT     Sequence Label: pEMBL-Si-LMNA
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(25..46)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(181..693,694..696,697..699))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     374..393
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     633..654
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      696..705
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     724..740
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     724..740
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gccgagagtg ancccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc
       61 gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg gcagcagcac
      121 ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgctgccgtc
      181 ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct tgtcggccat
      241 gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga tgttgccgtc
      301 ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc cctcgaactt
      361 cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc gctcctggac
      421 gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt cggggtagcg
      481 gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg gcacgggcag
      541 cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat cgccctcgcc
      601 ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga ccaggatggg
      661 caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg gtggatcggc
      721 cgctctagaa ctagtggatc ccccgggctg caggaattaa aaaacactta cgctgagtac
      781 ttcgatctct tgaatcgaag tactcagcgt aagggcccaa annnggnttt tctccnaggg
      841 anatttntag tctcaaaaca canc
//
