LOCUS       sequence-26571-e         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  TITLE     Unpublished Plasmids
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 1352wUV2-kpn1-bamH1-stop
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             197..466
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             476..499
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cctcccaatt caagtgcgga caacaaccct ttgcaacagc aatcatcaca aaataccgta
       61 ccaaacgtcc tcaaccaaat taaccaaatc ttttctccag aggagcaacg cagcttatta
      121 caagaagcca tcgaaacctg caagaatttt gaaaaaacac aattgtaagt ctcggctcca
      181 agaaggagat atacccatgg cacgcgtaac tgttcaggac gctgtagaga aaattggtaa
      241 ccgttttgac ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa
      301 ggacccgctc gtaccggaag aaaacgataa aaccactgta atcgcgctgc gcgaaatcga
      361 agaaggtctg atcaacaacc agatcctcga cgttcgcgaa cgccaggaac agcaagagca
      421 ggaagccgct gaattacaag ccgttaccgc tattgctgaa ggtcgtgcgg ccgcggacta
      481 caaggatgac gacgacaagt tccggaccgg ttccaagaca cccccccatg gtacccagcc
      541 ctataaatgt ccggagtgtg gcaagtcctt ctcgctaaag ggcaacctca cccgccacca
      601 acgtacgcac accggcgaaa aacctcatgc ttgccctgtc gagtcctgtg accgccgctt
      661 cagcacgagt ggcaacctca cccgccatat ccggattcac acaggcggtc agaagccctt
      721 ccagtgtcgc atctgcatgc gtaacttcag
//
