LOCUS       sequence-26575-e         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R
  TITLE     Efficient method to generate single-copy transgenic mice by 
            site-specific integration in embryonic stem cells.
  JOURNAL   Genesis. 2006 Jan . 44(1):23-8.
  PUBMED    16400644
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genesis. 
            2006 Jan . 44(1):23-8."
COMMENT     Sequence Label: Col1A-5'probe
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(479..497)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(518..534)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(518..534)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(531..553)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    542..558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(566..572,573..590,591..596))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    611..632
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggcggccgct ctagaactag tggatccccc gggctgcagg cccccgggta agttgcatct
       61 tccattactt cttcctgggt tcctccctgt cttggttact ttcagatatc cctctgttct
      121 ccagagaaga gttcagagac cagacagggg gtggggagaa aaagaagctt ggtcttacag
      181 aacctttgac cttcccaccc gtgccccctt gcaaacctgg ccataggaac tagttgaggg
      241 caccctctga gagacagaag cagagactgg gcggagccag cagtgggggg aggggaaaca
      301 cttctgagcc ggagtttggg ggctgccttg agtttctagc cactttttct aaccatctct
      361 ctatccctag ggtccccctg gctctgctgg ttctcctggc aaagacggac tcaacggtct
      421 ccctggcccc attggtcccc ctggtcctcg agggggggcc cggtaccagc ttttgttccc
      481 tttagtgagg gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
      541 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      601 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      661 agtcgggaaa
//
