LOCUS       sequence-265753-       13315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For Tol2 transposon-mediated stable expression of LRL, c-Myc and 
            IRES-luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13315)
  AUTHORS   Kawauchi D, Ogg RJ, Liu L, Shih DJH, Finkelstein D, Murphy BL, Rehg 
            JE, Korshunov A, Calabrese C, Zindy F, Phoenix T, Kawaguchi Y, 
            Gronych J, Gilbertson RJ, Lichter P, Gajjar A, Kool M, Northcott PA,
            Pfister SM, Roussel MF
  TITLE     Novel MYC-driven medulloblastoma models from multiple embryonic 
            cerebellar cells.
  JOURNAL   Oncogene. 2017 Sep 14;36(37):5231-5242. doi: 10.1038/onc.2017.110. 
            Epub 2017 May 15.
  PUBMED    28504719
REFERENCE   2  (bases 1 to 13315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/onc.2017.110"; journalName: "Oncogene"; date: "2017-09-14- 
            14"; volume: "36"; issue: "37"; pages: "5231-5242"
COMMENT     Sequence Label: pT2K-LRL-mouse c-MYC-IRES-Luc2
FEATURES             Location/Qualifiers
     source          1..13315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(34..462)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(144..165)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     356..375
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     589..611
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     603..620
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     604..620
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     630..649
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        630..648
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2499..2510,2511..2673)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     enhancer        3046..3425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          3700..4720
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4640..4662
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4728..4747
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(4793..4826)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4843..5520
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(5023..5043)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     5435..5458
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    5593..5648
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6009..6025)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6033..6049
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6057..6063,6064..6081,6082..6087))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6102..6123
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6182..6377
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6228..6363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    complement(6386..6419)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7815..8364
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7959..7976)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8186..8205
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8367..10019
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    10122..10177
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    join(10845..11032,11033..11044)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(11078..11094)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11078..11094)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(11131..11151)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11131..11149)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11170..11186)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11194..11210
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11218..11224,11225..11242,11243..11248))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11263..11284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11401..11418)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11572..12160)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11652..11671)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12331..13122,13123..13191))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12954..12973
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13192..13296)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      241 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      301 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      361 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      421 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
      481 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
      541 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
      601 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
      661 ccaaatagta ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt
      721 tgccattttc tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct
      781 tgaataaatg ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca
      841 ataatatgta atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga
      901 gaactggacc gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca
      961 aaatgaagtt aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga
     1021 cgttgccaat aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc
     1081 aacccctcta accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc
     1141 gggctgcaca aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt
     1201 aaggcatctg gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta
     1261 ttacagaaat tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca
     1321 ctggagcatt catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca
     1381 ctcattacag ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt
     1441 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     1501 gattggtagt caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca
     1561 tacataaaaa actggggggg ggggggatac aacaacacac ttaagtcaca tttgcctacg
     1621 taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt
     1681 aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt
     1741 ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca
     1801 caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga
     1861 ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag
     1921 actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga
     1981 agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt
     2041 ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt
     2101 attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc
     2161 ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct
     2221 aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa
     2281 tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc
     2341 caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc
     2401 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
     2461 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
     2521 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
     2581 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
     2641 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
     2701 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
     2761 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
     2821 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
     2881 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
     2941 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
     3001 agagactccc tggtgtctga aacacaggcc agatgggccc tcgtcgacat tgattattga
     3061 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3421 ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
     3481 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
     3541 gggggggcgc caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt
     3601 gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg
     3661 cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt
     3721 cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
     3781 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
     3841 gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag
     3901 ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc
     3961 gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
     4021 tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     4081 gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg
     4141 ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag
     4201 cacggcccgg cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg
     4261 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     4321 ggctcggggg aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc
     4381 cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa
     4441 atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg
     4501 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
     4561 cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg
     4621 gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagctagagc
     4681 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     4741 tattgtgctg tctcatcatt ttggcaaaga attcctcgag tctagatcgc gtataacttc
     4801 gtatagcata cattatacga agttatctcg agtctaggat ccatggcctc ctccgagaac
     4861 gtcatcaccg agttcatgcg cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag
     4921 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gccacaacac cgtgaagctg
     4981 aaggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
     5041 tacggctcca aggtgtacgt gaagcacccc gccgacatcc ccgactacaa gaagctgtcc
     5101 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggcgacc
     5161 gtgacccagg actcctccct gcaggacggc tgcttcatct acaaggtgaa gttcatcggc
     5221 gtgaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg ggaggcctcc
     5281 accgagcgcc tgtacccccg cgacggcgtg ctgaagggcg agacccacaa ggccctgaag
     5341 ctgaaggacg gcggccacta cctggtggag ttcaagtcca tctacatggc caagaagccc
     5401 gtgcagctgc ccggctacta ctacgtggac gccaagctgg acatcacctc ccacaacgag
     5461 gactacacca tcgtggagca gtacgagcgc accgagggcc gccaccacct gttcctgtag
     5521 gaattgatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     5581 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     5641 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     5701 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     5761 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     5821 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     5881 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     5941 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     6001 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6061 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6121 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     6181 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6241 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6301 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6361 gcctaggctt ttgcaaaaag ctaacataac ttcgtatagc atacattata cgaagttatg
     6421 agctcgaatt cactagtgat tggatccacg acgatgcccc tcaacgtgaa cttcaccaac
     6481 aggaactatg acctcgacta cgactccgta cagccctatt tcatctgcga cgaggaagag
     6541 aatttctatc accagcaaca gcagagcgag ctgcagccgc ccgcgcccag tgaggatatc
     6601 tggaagaaat tcgagctgct tcccaccccg cccctgtccc cgagccgccg ctccgggctc
     6661 tgctctccat cctatgttgc ggtcgctacg tccttctccc caagggaaga cgatgacggc
     6721 ggcggtggca acttctccac cgccgatcag ctggagatga tgaccgagtt acttggagga
     6781 gacatggtga accagagctt catctgcgat cctgacgacg agaccttcat caagaacatc
     6841 atcatccagg actgtatgtg gagcggtttc tcagccgctg ccaagctggt ctcggagaag
     6901 ctggcctcct accaggctgc gcgcaaagac agcaccagcc tgagccccgc ccgcgggcac
     6961 agcgtctgct ccacctccag cctgtacctg caggacctca ccgccgccgc gtccgagtgc
     7021 attgacccct cagtggtctt tccctacccg ctcaacgaca gcagctcgcc caaatcctgt
     7081 acctcgtccg attccacggc cttctctcct tcctcggact cgctgctgtc ctccgagtcc
     7141 tccccacggg ccagccctga gcccctagtg ctgcatgagg agacaccgcc caccaccagc
     7201 agcgactctg aagaagagca agaagatgag gaagaaattg atgtggtgtc tgtggagaag
     7261 aggcaaaccc ctgccaagag gtcggagtcg ggctcatctc catcccgagg ccacagcaaa
     7321 cctccgcaca gcccactggt cctcaagagg tgccacgtct ccactcacca gcacaactac
     7381 gccgcacccc cctccacaag gaaggactat ccagctgcca agagggccaa gttggacagt
     7441 ggcagggtcc tgaagcagat cagcaacaac cgcaagtgct ccagccccag gtcctcagac
     7501 acggaggaaa acgacaagag gcggacacac aacgtcttgg aacgtcagag gaggaacgag
     7561 ctgaagcgca gcttttttgc cctgcgtgac cagatccctg aattggaaaa caacgaaaag
     7621 gcccccaagg tagtgatcct caaaaaagcc accgcctaca tcctgtccat tcaagcagac
     7681 gagcacaagc tcacctctga aaaggactta ttgaggaaac gacgagaaca gttgaaacac
     7741 aaactcgaac agcttcgaaa ctctggtgca taaactgacc taactcgagc gggatcaatt
     7801 ccgccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     7861 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     7921 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     7981 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     8041 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     8101 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     8161 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     8221 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
     8281 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     8341 tttcctttga aaaacacgat aataccatgg aagatgccaa aaacattaag aagggcccag
     8401 cgccattcta cccactcgaa gacgggaccg ccggcgagca gctgcacaaa gccatgaagc
     8461 gctacgccct ggtgcccggc accatcgcct ttaccgacgc acatatcgag gtggacatta
     8521 cctacgccga gtacttcgag atgagcgttc ggctggcaga agctatgaag cgctatgggc
     8581 tgaatacaaa ccatcggatc gtggtgtgca gcgagaatag cttgcagttc ttcatgcccg
     8641 tgttgggtgc cctgttcatc ggtgtggctg tggccccagc taacgacatc tacaacgagc
     8701 gcgagctgct gaacagcatg ggcatcagcc agcccaccgt cgtattcgtg agcaagaaag
     8761 ggctgcaaaa gatcctcaac gtgcaaaaga agctaccgat catacaaaag atcatcatca
     8821 tggatagcaa gaccgactac cagggcttcc aaagcatgta caccttcgtg acttcccatt
     8881 tgccacccgg cttcaacgag tacgacttcg tgcccgagag cttcgaccgg gacaaaacca
     8941 tcgccctgat catgaacagt agtggcagta ccggattgcc caagggcgta gccctaccgc
     9001 accgcaccgc ttgtgtccga ttcagtcatg cccgcgaccc catcttcggc aaccagatca
     9061 tccccgacac cgctatcctc agcgtggtgc catttcacca cggcttcggc atgttcacca
     9121 cgctgggcta cttgatctgc ggctttcggg tcgtgctcat gtaccgcttc gaggaggagc
     9181 tattcttgcg cagcttgcaa gactataaga ttcaatctgc cctgctggtg cccacactat
     9241 ttagcttctt cgctaagagc actctcatcg acaagtacga cctaagcaac ttgcacgaga
     9301 tcgccagcgg cggggcgccg ctcagcaagg aggtaggtga ggccgtggcc aaacgcttcc
     9361 acctaccagg catccgccag ggctacggcc tgacagaaac aaccagcgcc attctgatca
     9421 cccccgaagg ggacgacaag cctggcgcag taggcaaggt ggtgcccttc ttcgaggcta
     9481 aggtggtgga cttggacacc ggtaagacac tgggtgtgaa ccagcgcggc gagctgtgcg
     9541 tccgtggccc catgatcatg agcggctacg ttaacaaccc cgaggctaca aacgctctca
     9601 tcgacaagga cggctggctg cacagcggcg acatcgccta ctgggacgag gacgagcact
     9661 tcttcatcgt ggaccggctg aagagcctga tcaaatacaa gggctaccag gtagccccag
     9721 ccgaactgga gagcatcctg ctgcaacacc ccaacatctt cgacgccggg gtcgccggcc
     9781 tgcccgacga cgatgccggc gagctgcccg ccgcagtcgt cgtgctggaa cacggtaaaa
     9841 ccatgaccga gaaggagatc gtggactatg tggccagcca ggttacaacc gccaagaagc
     9901 tgcgcggtgg tgttgtgttc gtggacgagg tgcctaaagg actgaccggc aagttggacg
     9961 cccgcaagat ccgcgagatt ctcattaagg ccaagaaggg cggcaagatc gccgtgtagg
    10021 gcggccgcga ctctagagtc gagtctagat atcagatctt tttccctctg ccaaaaatta
    10081 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
    10141 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
    10201 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
    10261 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
    10321 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
    10381 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
    10441 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
    10501 atccctcgac ctgcagccca agcttctgcg aagatacggc cacgggtgct cttgatcctg
    10561 tggctgattt tggactgtgc tgctcgcagc tgctgatgaa tcacatactt cctccatttt
    10621 cttccactga ttgactgtta taatttccct aatttccagg tcaaggtgct gtgcattgtg
    10681 gtaatagatg tgacatgacg tcacttccaa aggaccaatg aacatgtctg accaatttca
    10741 tataatgtga aaacgatttt cataggcaga ataaataaca tttaaattaa actgggcatc
    10801 agcgcaattc aattggtttg gtaatagcaa gggaaaatag aatgaagtga tctccaaaaa
    10861 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
    10921 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
    10981 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
    11041 tctggttctt gaccccctac cttcagcaag cccagcagat ccactagttc tagagcggcc
    11101 gccaccgcgg tggagctcca gcttttgttc cctttagtga gggttaattg cgcgcttggc
    11161 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
    11221 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
    11281 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
    11341 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
    11401 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
    11461 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
    11521 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
    11581 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
    11641 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
    11701 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
    11761 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
    11821 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
    11881 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
    11941 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
    12001 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
    12061 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
    12121 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
    12181 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
    12241 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
    12301 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
    12361 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
    12421 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
    12481 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
    12541 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
    12601 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
    12661 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
    12721 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
    12781 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
    12841 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
    12901 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
    12961 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
    13021 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
    13081 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
    13141 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
    13201 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
    13261 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtg
//
