LOCUS       sequence-265757-        8455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conjugative vector with streamlined cloning capabilities. Contains 
            two cloning sites for modular insertion of genetic parts..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY
FEATURES             Location/Qualifiers
     source          1..8455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(6..797)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    1003..1024
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     promoter        1025..1127
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1128..1787
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1194..1213)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     misc_feature    1839..1859
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(2060..2911)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(2898..3737)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(4248..5219)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            6458..6545
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      6887..7281
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     CDS             complement(7467..8282)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7467..8282)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8190..8209
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(8283..8374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8400..8417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agacattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
       61 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
      121 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
      181 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
      241 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
      301 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
      361 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
      421 gtggctggct cgaagatacc tgcaagaatg tcattgcgct gccattctcc aaattgcagt
      481 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
      541 acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
      601 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
      661 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
      721 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
      781 atcaccgctt ccctcatgat gtttaacttt gttttagggc gactgccctg ctgcgtaaca
      841 tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg ctgcttggat
      901 gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa aaccgccact
      961 gcgccgttac caccgctgcg ttcggtcaag gttctggacc aggaattcgc ggccgcttct
     1021 agagtgatcg gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg
     1081 gcgtattttt tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
     1141 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
     1201 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
     1261 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
     1321 gcctgatgaa tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat
     1381 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
     1441 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
     1501 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
     1561 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
     1621 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
     1681 tgccgctggc gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc
     1741 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
     1801 gttattggtg cccttaaacg cctggtgcta cgcctgaata ctagtagcgg ccgctgcagg
     1861 agcagaagag catacatctg gaagcaaagc caggaaagcg gcctatggag ctgtgcggca
     1921 gcgctcagta ggcaattttt caaaatattg ttaagccttt tctgagcatg gtatttttca
     1981 tggtattacc aattagcagg aaaataagcc attgaatata aaagataaaa atgtcttgtt
     2041 tacaatagag tggggggggt cagcctgccg ccttgggccg ggtgatgtcg tacttgcccg
     2101 ccgcgaactc ggttaccgtc cagcccagcg cgaccagctc cggcaacgcc tcgcgcaccc
     2161 gctggcggcg cttgcgcatg gtcgaaccac tggcctctga cggccagaca tagccgcaca
     2221 aggtatctat ggaagccttg ccggttttgc cggggtcgat ccagccacac agccgctggt
     2281 gcagcaggcg ggcggtttcg ctgtccagcg cccgcacctc gtccatgctg atgcgcacat
     2341 gctggccgcc acccatgacg gcctgcgcga tcaaggggtt cagggccacg tacaggcgcc
     2401 cgtccgcctc gtcgctggcg tactccgaca gcagccgaaa cccctgccgc ttgcggccat
     2461 tctgggcgat gatggatacc ttccaaaggc gctcgatgca gtcctgtatg tgcttgagcg
     2521 ccccaccact atcgacctct gccccgattt cctttgccag cgcccgatag ctacctttga
     2581 ccaccatggc atcagcggtg acggcctccc acttgggttc caggaacagc cggagctgcc
     2641 gtccgccttc ggtcttgggt tccgggccaa gcactaggcc attaggccca gccatggcca
     2701 ccagcccttg caggatgcgc agatcatcag cgcccagcgg ctccgggccg ctgaactcga
     2761 tccgcttgcc gtcgccgtag tcatacgtca cgtccagctt gctgcgcttg cgctcgcccc
     2821 gcttgagggc acggaacagg ccgggggcca gacagtgcgc cgggtcgtgc cggacgtggc
     2881 tgaggctgtg cttgttctta ggcttcacca cggggcaccc ccttgctctt gcgctgcctc
     2941 tccagcacgg cgggcttgag caccccgccg tcatgccgcc tgaaccaccg atcagcgaac
     3001 ggtgcgccat agttggcctt gctcacaccg aagcggacga agaaccggcg ctggtcgtcg
     3061 tccacacccc attcctcggc ctcggcgctg gtcatgctcg acaggtagga ctgccagcgg
     3121 atgttatcga ccagtaccga gctgccccgg ctggcctgct gctggtcgcc tgcgcccatc
     3181 atggccgcgc ccttgctggc atggtgcagg aacacgatag agcacccggt atcggcggcg
     3241 atggcctcca tgcgaccgat gacctgggcc atggggccgc tggcgttttc ttcctcgatg
     3301 tggaaccggc gcagcgtgtc cagcaccatc aggcggcggc cctcggcggc gcgcttgagg
     3361 ccgtcgaacc actccggggc catgatgttg ggcaggctgc cgatcagcgg ctggatcagc
     3421 aggccgtcag ccacggcttg ccgttcctcg gcgctgaggt gcgccccaag ggcgtgcagg
     3481 cggtgatgaa tggcggtggg cgggtcttcg gcgggcaggt agatcaccgg gccggtgggc
     3541 agttcgccca cctccagcag atccggcccg cctgcaatct gtgcggccag ttgcagggcc
     3601 agcatggatt taccggcacc accgggcgac accagcgccc cgaccgtacc ggccaccatg
     3661 ttgggcaaaa cgtagtccag cggtggcggc gctgctgcga acgcctccag aatattgata
     3721 ggcttatggg tagccattga ttgcctcctt tgcaggcagt tggtggttag gcgctggcgg
     3781 ggtcactacc cccgccctgc gccgctctga gttcttccag gcactcgcgc agcgcctcgt
     3841 attcgtcgtc ggtcagccag aacttgcgct gacgcatccc tttggccttc atgcgctcgg
     3901 catatcgcgc ttggcgtaca gcgtcagggc tggccagcag gtcgccggtc tgcttgtcct
     3961 tttggtcttt catatcagtc accgagaaac ttgccggggc cgaaaggctt gtcttcgcgg
     4021 aacaaggaca aggtgcagcc gtcaaggtta aggctggcca tatcagcgac tgaaaagcgg
     4081 ccagcctcgg ccttgtttga cgtataacca aagccaccgg gcaaccaata gcccttgtca
     4141 cttttgatca ggtagaccga ccctgaagcg cttttttcgt attccataaa acccccttct
     4201 gtgcgtgagt actcatagta taacaggcgt gagtaccaac gcaagcacta catgctgaaa
     4261 tctggcccgc ccctgtccat gcctcgctgg cggggtgccg gtgcccgtgc cagctcggcc
     4321 cgcgcaagct ggacgctggg cagacccatg accttgctga cggtgcgctc gatgtaatcc
     4381 gcttcgtggc cgggcttgcg ctctgccagc gctgggctgg cctcggccat ggccttgccg
     4441 atttcctcgg cactgcggcc ccggctggcc agcttctgcg cggcgataaa gtcgcacttg
     4501 ctgaggtcat caccgaagcg cttgaccagc ccggccatct cgctgcggta ctcgtccagc
     4561 gccgtgcgcc ggtggcggct aagctgccgc tcgggcagtt cgaggctggc cagcctgcgg
     4621 gccttctcct gctgccgctg ggcctgctcg atctgctggc cagcctgctg caccagcgcc
     4681 gggccagcgg tggcggtctt gcccttggat tcacgcagca gcacccacgg ctgataaccg
     4741 gcgcgggtgg tgtgcttgtc cttgcggttg gtgaagcccg ccaagcggcc atagtggcgg
     4801 ctgtcggcgc tggccgggtc ggcgtcgtac tcgctggcca gcgtccgggc aatctgcccc
     4861 cgaagttcac cgcctgcggc gtcggccacc ttgacccatg cctgatagtt cttcgggctg
     4921 gtttccacta ccagggcagg ctcccggccc tcggctttca tgtcatccag gtcaaactcg
     4981 ctgaggtcgt ccaccagcac cagaccatgc cgctcctgct cggcgggcct gatatacacg
     5041 tcattgccct gggcattcat ccgcttgagc catggcgtgt tctggagcac ttcggcggct
     5101 gaccattccc ggttcatcat ctggccggtg gtggcgtccc tgacgccgat atcgaagcgc
     5161 tcacagccca tggccttgag ctgtcggcct atggcctgca aagtcctgtc gttcttcatc
     5221 gggccaccaa gcgcagccag atcgagccgt cctcggttgt cagtggcgtc aggtcgagca
     5281 agagcaacga tgcgatcagc agcaccaccg taggcatcat ggaagccagc atcacggtta
     5341 gccatagctt ccagtgccac ccccgcgacg cgctccgggc gctctgcgcg gcgctgctca
     5401 cctcggcggc tacctcccgc aactctttgg ccagctccac ccatgccgcc cctgtctggc
     5461 gctgggcttt cagccactcc gccgcctgcg cctcgctggc ctgctgggtc tggctcatga
     5521 cctgccgggc ttcgtcggcc agtgtcgcca tgctctgggc cagcggttcg atctgctccg
     5581 ctaactcgtt gatgcctctg gatttcttca ctctgtcgat tgcgttcatg gtctattgcc
     5641 tcccggtatt cctgtaagtc gatgatctgg gcgttggcgg tgtcgatgtt cagggccacg
     5701 tctgcccggt cggtgcggat gccccggcct tccatctcca ccacgttcgg ccccaggtga
     5761 acaccgggca ggcgctcgat gccctgcgcc tcaagtgttc tgtggtcaat gcgggcgtcg
     5821 tggccagccc gctctaatgc ccggttggca tggtcggccc atgcctcgcg ggtctgctca
     5881 agccatgcct tgggcttgag cgcttcggtc ttctgtgccc cgcccttctc cggggtcttg
     5941 ccgttgtacc gcttgaacca ctgagcggcg ggccgctcga tgccgtcatt gatccgctcg
     6001 gagatcatca ggtggcagtg cgggttctcg ccgccaccgg catggatggc cagcgtatac
     6061 ggcaggcgct cggcaccggt caggtgctgg gcgaactcgg acgccagcgc cttctgctgg
     6121 tcgagggtca gctcgaccgg cagggcaaat tcgacctcct tgaacagccg cccattggcg
     6181 cgttcataca ggtcggcagc atcccagtag tcggcgggcc gctcgacgaa ctccggcatg
     6241 tgcccggatt cggcgtgcaa gacttcatcc atgtcgcggg catacttgcc ttcgcgctgg
     6301 atgaagtcgg ccttggccct ggccgattgg ccgcccgacc tgctgccggt tttcgccgta
     6361 aggtgataaa tcgccatgct gcctcgctgt tgcttttgct tttcggctcc atgcaatggc
     6421 cctcggagag cgcaccgccc gaagggtggc cgttaggcca gtttctcgaa gagaaaccgg
     6481 taagtgcgcc ctcccctaca aagtagggtc gggattgccg ccgctgtgcc tccatgatag
     6541 cctacgagac agcacattaa caatggggtg tcaagatggt taaggggagc aacaaggcgg
     6601 cggatcggct ggccaagctc gaagaacaac gagcgcgaat caatgccgaa attcagcggg
     6661 tgcgggcaag ggaacagcag caagagcgca agaacgaaac aaggcgcaag gtgctggtgg
     6721 gggccatgat tttggccaag gtgaacagca gcgagtggcc ggaggatcgg ctcatggcgg
     6781 caatggatgc gtaccttgaa cgcgaccacg accgcgcctt gttcggtctg ccgccacgcc
     6841 agaaggatga gccgggctga atgatcgacc gagacaggcc ctgcggggct gcacacgcgc
     6901 ccccaccctt cgggtagggg gaaaggccgc taaagcggct aaaagcgctc cagcgtattt
     6961 ctgcggggtt tggtgtgggg tttagcgggc tttgcccgcc tttccccctg ccgcgcagcg
     7021 gtggggcggt gtgtagccta gcgcagcgaa tagaccagct atccggcctc tggccgggca
     7081 tattgggcaa gggcagcagc gccccacaag ggcgctgata accgcgccta gtggattatt
     7141 cttagataat catggatgga tttttccaac accccgccag cccccgcccc tgctgggttt
     7201 gcaggtttgg gggcgtgaca gttattgcag gggttcgtga cagttattgc aggggggcgt
     7261 gacagttatt gcaggggttc gtgacagtta gtacgggagt gacgggggta ccgaggtcga
     7321 tgtcgactaa cgaccctgcc ctgaaccgac gacaagctga cgaccgggtc tccgcaagtg
     7381 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     7441 atatgtatcc gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc
     7501 aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa
     7561 ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt
     7621 ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca
     7681 agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt
     7741 tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca
     7801 accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg gtcgctgtta
     7861 aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca
     7921 acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg
     7981 atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga
     8041 agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca
     8101 acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga
     8161 tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc atataaatca
     8221 gcatccatgt tggaatttaa tcgcggccta gagcaagacg tttcccgttg aatatggctc
     8281 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8341 tacatatttg aatgtattta gaaaaataaa caaataggca tgcagcgctc ttccgcttcc
     8401 tcgctcactg actcgctacg ctcggtcgtt cgactctcga gagcggccga agctt
//
