LOCUS       sequence-265759-        1046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the activator rhaS, as well as rhamnose-inducible expression of 
            mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-Prha-mVenus_rhaS
FEATURES             Location/Qualifiers
     source          1..1046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      926..997
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1013..1040
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gccacaattc agcaaattgt gaacatcatc acgttcatct ttccctggtt gccaatggcc
       61 cattttcctg tcagtaacga gaaggtcgcg aattcaggcg ctttttagac tggtcgtaat
      121 gaatactaga gtagtggagg ttactagatg gctagcaaag gagaggaact gtttaccggt
      181 gtggtaccca ttttagtaga attggatggt gatgtgaacg gccacaagtt cagcgtttcc
      241 ggggaaggcg aaggggatgc aacctatgga aagctaactt tgaaactcat ttgcactacc
      301 ggtaaactcc ccgtcccctg gcctacgtta gtcacgaccc tcggttatgg tctgcaatgt
      361 tttgcccgtt atcccgatca tatgaaacaa cacgatttct ttaaatctgc catgccagag
      421 ggatatgttc aagaacggac aattttcttt aaggatgatg gtaattataa aacccgtgca
      481 gaagtcaaat ttgaaggtga tacactcgtg aatcgcatcg aactaaaagg tattgacttt
      541 aaggaggatg gaaacattct cggccataag ttagaataca attacaacag ccataatgtg
      601 tacattactg cggataaaca aaaaaacggt atcaaagcca actttaaaat tcgacataac
      661 atcgaagatg gtggcgttca gttagcggat cactaccaac aaaatactcc cattggagac
      721 ggaccagtcc tgctgcctga taaccattat ctgtcttatc aatccaaact gtccaaggat
      781 ccgaatgaaa agcgggatca catggttctg ttggagtttg tgaccgcggc aggtattact
      841 ctcggaatgg acgaactata caaaaggcct gccgccaacg acgaaaacta cgctgcatca
      901 gtttaatagt actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
      961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1021 ttcgggtggg cctttctgcg tttata
//
