LOCUS       sequence-265760-        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the activator rhaS, as well as rhamnose-inducible expression of 
            mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-Prha-mVenus_rhaS
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     RBS             36..47
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             54..890
                     /codon_start=1
                     /gene="E. coli rhaS"
                     /product="positive regulator of the rhaB promoter
                     "
                     /label=rhaS
                     /note="activated by L-rhamnose"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHGT
                     GIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLLPQ
                     ELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKSSLQ
                     ENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQLYLNR
                     LRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGFLQ"
     terminator      935..1006
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1022..1049
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcaaaga ggagaaatac tagatgaccg
       61 tattacatag tgtggatttt tttccgtctg gtaacgcgtc cgtggcgata gaaccccggc
      121 tcccgcaggc ggattttcct gaacatcatc atgattttca tgaaattgtg attgtcgaac
      181 atggcacggg tattcatgtg tttaatgggc agccctatac catcaccggt ggcacggtct
      241 gtttcgtacg cgatcatgat cggcatctgt atgaacatac cgataatctg tgtctgacca
      301 atgtgctgta tcgctcgccg gatcgatttc agtttctcgc cgggctgaat cagttgctgc
      361 cacaagagct ggatgggcag tatccgtctc actggcgcgt taaccacagc gtattgcagc
      421 aggtgcgaca gctggttgca cagatggaac agcaggaagg ggaaaatgat ttaccctcga
      481 ccgccagtcg cgagatcttg tttatgcaat tactgctctt gctgcgtaaa agcagtttgc
      541 aggagaacct ggaaaacagc gcatcacgtc tcaacttgct tctggcctgg ctggaggacc
      601 attttgccga tgaggtgaat tgggatgccg tggcggatca attttctctt tcactgcgta
      661 cgctacatcg gcagcttaag cagcaaacgg gactgacgcc tcagctatac ctgaaccgcc
      721 tgcgactgat gaaagcccga catctgctac gccacagcga ggccagcgtt actgacatcg
      781 cctatcgctg tggattcagc gacagtaacc acttttcgac gctttttcgc cgagagttta
      841 actggtcacc gcgtgatatt cgccagggac gggatggctt tctgcaataa taacactgat
      901 agtgctagtg tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      961 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1021 tggctcacct tcgggtgggc ctttctgcgt ttata
//
