LOCUS       sequence-265767-         709 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the repressor tetR, as well as aTc-inducible expression of mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-PL03-mVenus_tetR
FEATURES             Location/Qualifiers
     source          1..709
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     CDS             52..675
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(125..144)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      676..703
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcaagag agaaatacta gatgtccaga
       61 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
      121 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
      181 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
      241 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
      301 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
      361 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
      421 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
      481 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
      541 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
      601 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
      661 gaaagtgggt cctaaggctc accttcgggt gggcctttct gcgtttata
//
