LOCUS       sequence-265769-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the repressor vanR, as well as vanillate-inducible expression of 
            mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-PvanCC-mVenus_vanR
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        54..103
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      929..1000
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1016..1043
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 gattggatcc aattgacagc tagctcagtc ctaggtacca ttggatccaa tagctgtcac
       61 cggatgtgct ttccggtctg atgagtccgt gaggacgaaa cagcctctac aaataatttt
      121 gtttaatact agagtagtgg aggttactag atggctagca aaggagagga actgtttacc
      181 ggtgtggtac ccattttagt agaattggat ggtgatgtga acggccacaa gttcagcgtt
      241 tccggggaag gcgaagggga tgcaacctat ggaaagctaa ctttgaaact catttgcact
      301 accggtaaac tccccgtccc ctggcctacg ttagtcacga ccctcggtta tggtctgcaa
      361 tgttttgccc gttatcccga tcatatgaaa caacacgatt tctttaaatc tgccatgcca
      421 gagggatatg ttcaagaacg gacaattttc tttaaggatg atggtaatta taaaacccgt
      481 gcagaagtca aatttgaagg tgatacactc gtgaatcgca tcgaactaaa aggtattgac
      541 tttaaggagg atggaaacat tctcggccat aagttagaat acaattacaa cagccataat
      601 gtgtacatta ctgcggataa acaaaaaaac ggtatcaaag ccaactttaa aattcgacat
      661 aacatcgaag atggtggcgt tcagttagcg gatcactacc aacaaaatac tcccattgga
      721 gacggaccag tcctgctgcc tgataaccat tatctgtctt atcaatccaa actgtccaag
      781 gatccgaatg aaaagcggga tcacatggtt ctgttggagt ttgtgaccgc ggcaggtatt
      841 actctcggaa tggacgaact atacaaaagg cctgccgcca acgacgaaaa ctacgctgca
      901 tcagtttaat agtactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
      961 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1021 accttcgggt gggcctttct gcgtttatat
//
