LOCUS       sequence-265770-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Conjugative vector derived from pSHDY. Constitutive expression of 
            the repressor vanR, as well as vanillate-inducible expression of 
            mVenus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 35)
  AUTHORS   Behle A, Saake P, Germann AT, Dienst D, Axmann IM
  TITLE     Comparative Dose-Response Analysis of Inducible Promoters in 
            Cyanobacteria.
  JOURNAL   ACS Synth Biol. 2020 Apr 17;9(4):843-855. doi: 
            10.1021/acssynbio.9b00505. Epub 2020 Mar 17.
  PUBMED    32134640
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.9b00505"; journalName: "ACS Synth Biol"; date: 
            "2020-04-17- 17"; volume: "9"; issue: "4"; pages: "843-855"
COMMENT     Sequence Label: pSHDY-PvanCC-mVenus_vanR
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..35
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      806..833
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagcgctta aactaacgaa cgtaaataag
       61 gaggatagac atggacatgc ctcgtattaa accgggtcag cgtgttatga tggcactgcg
      121 taaaatgatt gcaagcggtg aaatcaaaag tggtgaacgt attgcagaaa ttccgaccgc
      181 agcagcactg ggtgttagcc gtatgccggt tcgtatcgca ctgcgttcac tggaacaaga
      241 aggtctggtt gttcgtctgg gtgcacgtgg ttatgcagcc cgtggtgtta gcagcgatca
      301 gattcgtgat gcaattgaag ttcgtggtgt tctggaaggt tttgcagcac gtcgtctggc
      361 agaacgtggt atgaccgcag aaacccatgc acgttttgtt gtactgattg cagaaggtga
      421 agcactgttt gcagccggtc gcctgaatgg tgaagatctg gatcgttatg ccgcatataa
      481 tcaggcattt catgataccc tggttagcgc agcaggtaat ggtgcagttg aaagcgcact
      541 ggcacgtaat ggttttgaac cgtttgcagc agccggtgca ctggccctgg atctgatgga
      601 cctgtctgcc gaatatgaac atctgctggc agcacatcgt cagcatcagg cagttctgga
      661 tgcagttagc tgtggtgatg ccgaaggtgc agaacgtatt atgcgtgatc atgcactggc
      721 agcaattcgt aatgcaaaag tttttgaagc agcagcaagc gcaggcgcac cgctgggtgc
      781 agcatggtca attcgtgcag attgaggctc accttcgggt gggcctttct gcgtttata
//
