LOCUS       sequence-265773-        4005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221_SLC30A10
FEATURES             Location/Qualifiers
     source          1..4005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2130..2229)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2246..2265)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2247..2265)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2270..2286)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2270..2286)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2388..2407)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2399..3208
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2466..2485)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3355..3943
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3844..3863
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc accatgggcc gctactccgg caagacctgc aggctgctgt tcatgctggt
      721 gctgacagtg gccttctttg tggccgagct ggtgtccggc tatctgggca actctatcgc
      781 cctgctgtcc gactctttta atatgctgtc cgatctgatc tctctgtgcg tgggactgag
      841 cgccggatac atcgcacgga gacctaccag aggcttctcc gccacatacg gctatgcaag
      901 ggcagaggtg gtgggcgccc tgagcaacgc cgtgtttctg accgccctgt gcttcacaat
      961 ctttgtggag gccgtgctga ggctggcaag acctgagagg atcgacgatc cagagctggt
     1021 gctgatcgtg ggcgtgctgg gcctgctggt gaatgtggtg ggcctgctga tcttccagga
     1081 ctgcgcagca tggtttgcat gctgtctgag gggcagatcc aggcgcctgc agcagaggca
     1141 gcagctggcc gagggatgcg tgccaggagc cttcggcgga cctcagggcg ccgaggaccc
     1201 aaggagagca gcagatccaa ccgcaccagg aagcgattcc gccgtgaccc tgaggggcac
     1261 atctgtggag aggaagcgcg agaagggagc aaccgtgttc gcaaacgtgg caggcgactc
     1321 ctttaacaca cagaatgagc ctgaggatat gatgaagaag gagaagaagt ctgaggccct
     1381 gaatatcaga ggcgtgctgc tgcacgtgat gggcgacgca ctgggatccg tggtggtggt
     1441 catcacagcc atcatcttct acgtgctgcc cctgaagtct gaggaccctt gcaactggca
     1501 gtgttatatc gatccaagcc tgaccgtgct gatggtcatc atcatcctga gctccgcctt
     1561 tcctctgatc aaggagacag ccgccatcct gctgcagatg gtgccaaagg gcgtgaatat
     1621 ggaggagctg atgtctaagc tgagcgccgt gccaggcatc tctagcgtgc acgaggtgca
     1681 catctgggag ctggtgagcg gcaagatcat cgccaccctg cacatcaagt accccaagga
     1741 ccgcggctat caggatgcct ccaccaagat ccgggagatc ttccaccacg ccggcatcca
     1801 caacgtgaca atccagtttg agaatgtgga cctgaaggag ccactggagc agaaggatct
     1861 gctgctgctg tgcaacagcc cctgtatctc caagggatgc gcaaagcagc tgtgctgtcc
     1921 acctggcgcc ctgccactgg cacacgtgaa cggctgtgcc gagcacaatg gcggcccttc
     1981 tctggacacc tacggatctg atggactgag caggagggac gcaagagagg tggcaatcga
     2041 ggtgtccctg gactcttgcc tgagcgatca cggccagagc ctgaacaaga cccaggagga
     2101 tcagtgttat gtgaatagga cacactttga cccagctttc ttgtacaaag ttggcattat
     2161 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     2221 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt
     2281 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa
     2341 tatatcatca tgaacaataa aactgtctgc ttacataaac agtaatacaa ggggtgttat
     2401 gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg atgctgattt
     2461 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt
     2521 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2581 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2641 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatccccgg
     2701 aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2761 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2821 cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2881 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     2941 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3001 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3061 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3121 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3181 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3241 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3301 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3361 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3421 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3481 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3541 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3601 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3661 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3721 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3781 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3841 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     3901 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     3961 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
