LOCUS       sequence-265778-        9735 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pH measurement, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9735)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 9735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: pT2MP.EF-pHlu
FEATURES             Location/Qualifiers
     source          1..9735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(18..446)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(128..149)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     340..359
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     573..595
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     587..604
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     588..604
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     611..630
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        611..629
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(643..654,655..842)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2111..2159
                     /note="synthetic polyadenylation signal"
     promoter        2194..3376
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2425..3367
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3324..3344
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3386..3466
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      3468..3477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4247..4471
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4518..4541)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4915..4934
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4999..5598
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4999..5018)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5495..5515
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5685..5909
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5937..5953)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5961..5977
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5985..5991,5992..6009,6010..6015))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6030..6051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7367..7504,7505..7516)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7535..7553)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7574..7590)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7598..7614
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7622..7628,7629..7646,7647..7652))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7667..7688
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7805..7822)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7976..8564)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8056..8075)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8735..9526,9527..9595))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9358..9377
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9596..9700)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
       61 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
      121 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
      181 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
      241 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
      301 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
      361 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
      421 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
      481 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
      541 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
      601 cagtgaattg taatacgact cactataggg cgaattgggt accagaggtg taaagtactt
      661 gagtaatttt acttgattac tgtacttaag tattattttt ggggattttt actttacttg
      721 agtacaatta aaaatcaata cttttacttt tacttaatta cattttttta gaaaaaaaag
      781 tactttttac tccttacaat tttatttaca gtcaaaaagt acttattttt tggagatcac
      841 ttgtcgagta gggataacag ggtaatgatc ccccgggctg cagtcgcgac gcgtcctaga
      901 gggacagccc ccccccaaag cccccaggga tgtaattacg tccctccccc gctagggggc
      961 agcagcgagc cgcccggggc tccgctccgg tccggcgctc cccccgcatc cccgagccgg
     1021 cagcgtgcgg ggacagcccg ggcacgggga aggtggcacg ggatcgcttt cctctgaacg
     1081 cttctcgctg ctctttgagc ctgcagacac ctggggggat acggggaaaa agctttaggc
     1141 tgaaagagag atttagaatg acagaatcat agaacggcct gggttgcaaa ggagcacagt
     1201 gctcatccag atccaacccc ctgctatgtg cagggtcatc aaccagcagc ccaggctgcc
     1261 cagagccaca tccagcctgg ccttgaatgc ctgcagggat ggggcatcca cagcctcctt
     1321 gggcaacctg ttcagtgcgt caccaccctc tgggggaaaa actgcctcct catatccaac
     1381 ccaaacctcc cctgtctcag tgtaaagcca ttcccccttg tcctatcaag ggggagtttg
     1441 ctgtgacatt gttggtctgg ggtgacacat gtttgccaat tcagtgcatc acggagaggc
     1501 agatcttggg gataaggaag tgcaggacag catggacgtg ggacatgcag gtgttgaggg
     1561 ctctgggaca ctctccaagt cacagcgttc agaacagcct taaggataag aagataggat
     1621 agaaggacaa agagcaagtt aaaacccagc atggagagga gcacaaaaag gccacagaca
     1681 ctgctggtcc ctgtgtctga gcctgcatgt ttgatggtgt ctggatgcaa gcagaagggg
     1741 tggaagagct tgcctggaga gatacagctg ggtcagtagg actgggacag gcagctggag
     1801 aattgccatg tagatgttca tacaatcgtc aaatcatgaa ggctggaaaa gccctccaag
     1861 atccccaaga ccaaccccaa cccacccacc gtgcccactg gccatgtccc tcagtgccac
     1921 atccccacag ttcttcatca cctccaggga cggtgacccc cccacctccg tgggcagctg
     1981 tgccactgca gcaccgctct ttggagaagg taaatcttgc taaatccagc ccgaccctcc
     2041 cctggcacaa cgtaaggcca ttatctctca tccaactcca ggacggagtc agtgaggatg
     2101 gggctctagg aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtgg
     2161 tcgacattga ttattgacta gatcatcgcg tgaggctccg gtgcccgtca gtgggcagag
     2221 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     2281 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     2341 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     2401 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     2461 tttacgggtt atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt
     2521 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     2581 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     2641 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     2701 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     2761 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     2821 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     2881 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     2941 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     3001 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     3061 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     3121 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     3181 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     3241 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     3301 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     3361 atttcaggtg tcgtgaggaa ttaaagctag cgctaccgga ctcagatctc gagctcaagc
     3421 ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
     3481 gcaagggtga agagctgttt accggcgtgg tgccgattct ggtggaactg gatggtgacg
     3541 tgaacggcca caaattcagc gtgtccggcg aaggcgaggg cgatgccacc tatggtaaac
     3601 tgaccctgaa gtttatctgc accaccggca agctgccagt gccatggcca accctggtga
     3661 ccacctttag ctatggcgtg cagtgtttct cccgctaccc cgatcacatg aaacgtcatg
     3721 actttttcaa gagcgccatg ccagaaggtt atgtgcagga gcgcaccatt ttctttaaag
     3781 atgacggcaa ttacaagacc cgtgcggaag tgaaatttga gggcgatacc ctggtgaacc
     3841 gcattgaact gaaaggcatc gacttcaagg atgacggtaa tattctgggc cacaagctgg
     3901 aatataacta caatgagcat ctggtgtata tcatggcaga taaacagaag aacggcacca
     3961 aagctatttt ccaggtgcac cataatatcg aggatggcgg tgtgcagctg gcagaccatt
     4021 atcagcagaa cacccctatt ggcgatggcc ctgtgctgct gccggacaat cactacctgc
     4081 atacccagtc tgctctgagt aaagatccga acgaaaagcg cgaccacatg gtgctgctgg
     4141 agtttgtgac cgccgcgggt atcacccatg gcatggacga gctgtacaaa tgagcggccg
     4201 ctcgataagc ttgatcgatg atctagagct cgctgatcag cctcgactgt gccttctagt
     4261 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     4321 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     4381 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     4441 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac ctgcagcttt
     4501 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4561 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4621 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4681 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4741 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     4801 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     4861 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     4921 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     4981 cgacctgtgg ccacaaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5041 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5101 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5161 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5221 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5281 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5341 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     5401 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     5461 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     5521 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     5581 cgcaagcccg gtgcctgacg cccgccccac gacccgcagc gcccgaccga aaggagcgca
     5641 cgaccccatg catcgatgat ctagagctcg ctgatcagcc tcgactgtgc cttctagttg
     5701 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     5761 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     5821 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     5881 gcatgctggg gatgcggtgg gctctatggc tgcagcccaa gcttggcgta atcatggtca
     5941 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6001 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6061 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatccc tagagggaca
     6121 gccccccccc aaagccccca gggatgtaat tacgtccctc ccccgctagg gggcagcagc
     6181 gagccgcccg gggctccgct ccggtccggc gctccccccg catccccgag ccggcagcgt
     6241 gcggggacag cccgggcacg gggaaggtgg cacgggatcg ctttcctctg aacgcttctc
     6301 gctgctcttt gagcctgcag acacctgggg ggatacgggg aaaaagcttt aggctgaaag
     6361 agagatttag aatgacagaa tcatagaacg gcctgggttg caaaggagca cagtgctcat
     6421 ccagatccaa ccccctgcta tgtgcagggt catcaaccag cagcccaggc tgcccagagc
     6481 cacatccagc ctggccttga atgcctgcag ggatggggca tccacagcct ccttgggcaa
     6541 cctgttcagt gcgtcaccac cctctggggg aaaaactgcc tcctcatatc caacccaaac
     6601 ctcccctgtc tcagtgtaaa gccattcccc cttgtcctat caagggggag tttgctgtga
     6661 cattgttggt ctggggtgac acatgtttgc caattcagtg catcacggag aggcagatct
     6721 tggggataag gaagtgcagg acagcatgga cgtgggacat gcaggtgttg agggctctgg
     6781 gacactctcc aagtcacagc gttcagaaca gccttaagga taagaagata ggatagaagg
     6841 acaaagagca agttaaaacc cagcatggag aggagcacaa aaaggccaca gacactgctg
     6901 gtccctgtgt ctgagcctgc atgtttgatg gtgtctggat gcaagcagaa ggggtggaag
     6961 agcttgcctg gagagataca gctgggtcag taggactggg acaggcagct ggagaattgc
     7021 catgtagatg ttcatacaat cgtcaaatca tgaaggctgg aaaagccctc caagatcccc
     7081 aagaccaacc ccaacccacc caccgtgccc actggccatg tccctcagtg ccacatcccc
     7141 acagttcttc atcacctcca gggacggtga cccccccacc tccgtgggca gctgtgccac
     7201 tgcagcaccg ctctttggag aaggtaaatc ttgctaaatc cagcccgacc ctcccctggc
     7261 acaacgtaag gccattatct ctcatccaac tccaggacgg agtcagtgag gatggggctc
     7321 tagccggacc gacgcgtctc gatagggata acagggtaat tgatccaata ctcaagtaca
     7381 attttaatgg agtacttttt tacttttact caagtaagat tctagccaga tacttttact
     7441 tttaattgag taaaattttc cctaagtact tgtactttca cttgagtaaa atttttgagt
     7501 actttttaca cctctggagc tccagctttt gttcccttta gtgagggtta atttcgagct
     7561 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     7621 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     7681 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     7741 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     7801 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     7861 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     7921 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     7981 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     8041 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     8101 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     8161 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     8221 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     8281 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     8341 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     8401 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     8461 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     8521 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     8581 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     8641 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     8701 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     8761 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     8821 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     8881 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     8941 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9001 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9061 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     9121 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     9181 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     9241 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     9301 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     9361 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     9421 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     9481 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacgggaa
     9541 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     9601 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     9661 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     9721 cacctaaatt gtaag
//
