LOCUS       sequence-265781-       10115 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QUEEN ATP sensor for 25degC, Tol2 transposon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10115)
  AUTHORS   Yaginuma H, Kawai S, Tabata KV, Tomiyama K, Kakizuka A, Komatsuzaki 
            T, Noji H, Imamura H
  TITLE     Diversity in ATP concentrations in a single bacterial cell 
            population revealed by quantitative single-cell imaging.
  JOURNAL   Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038/srep06522.
  PUBMED    25283467
REFERENCE   2  (bases 1 to 10115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2014-10-6"; volume: "4:6522. doi"; pages: " 
            10.1038/srep06522"
COMMENT     Sequence Label: pT2MP.EF-Q2m
FEATURES             Location/Qualifiers
     source          1..10115
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(655..666,667..854)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    2123..2171
                     /note="synthetic polyadenylation signal"
     promoter        2206..3388
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2437..3379
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3336..3356
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3434..3443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3800..4051
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     3988..4009
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4115..4136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4376..4395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     polyA_signal    4639..4863
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4910..4933)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5307..5326
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5391..5990
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5391..5410)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5887..5907
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    6077..6301
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6329..6345)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6353..6369
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6377..6383,6384..6401,6402..6407))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6422..6443
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    join(7759..7896,7897..7908)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     promoter        complement(7927..7945)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7966..7982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7990..8006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8014..8020,8021..8038,8039..8044))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8059..8080
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8197..8214)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8368..8956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8448..8467)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9127..9918,9919..9987))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9750..9769
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9988..10092)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccagagg
      661 tgtaaagtac ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt
      721 ttactttact tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt
      781 tagaaaaaaa agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt
      841 tttggagatc acttgtcgag tagggataac agggtaatga tcccccgggc tgcagtcgcg
      901 acgcgtccta gagggacagc ccccccccaa agcccccagg gatgtaatta cgtccctccc
      961 ccgctagggg gcagcagcga gccgcccggg gctccgctcc ggtccggcgc tccccccgca
     1021 tccccgagcc ggcagcgtgc ggggacagcc cgggcacggg gaaggtggca cgggatcgct
     1081 ttcctctgaa cgcttctcgc tgctctttga gcctgcagac acctgggggg atacggggaa
     1141 aaagctttag gctgaaagag agatttagaa tgacagaatc atagaacggc ctgggttgca
     1201 aaggagcaca gtgctcatcc agatccaacc ccctgctatg tgcagggtca tcaaccagca
     1261 gcccaggctg cccagagcca catccagcct ggccttgaat gcctgcaggg atggggcatc
     1321 cacagcctcc ttgggcaacc tgttcagtgc gtcaccaccc tctgggggaa aaactgcctc
     1381 ctcatatcca acccaaacct cccctgtctc agtgtaaagc cattccccct tgtcctatca
     1441 agggggagtt tgctgtgaca ttgttggtct ggggtgacac atgtttgcca attcagtgca
     1501 tcacggagag gcagatcttg gggataagga agtgcaggac agcatggacg tgggacatgc
     1561 aggtgttgag ggctctggga cactctccaa gtcacagcgt tcagaacagc cttaaggata
     1621 agaagatagg atagaaggac aaagagcaag ttaaaaccca gcatggagag gagcacaaaa
     1681 aggccacaga cactgctggt ccctgtgtct gagcctgcat gtttgatggt gtctggatgc
     1741 aagcagaagg ggtggaagag cttgcctgga gagatacagc tgggtcagta ggactgggac
     1801 aggcagctgg agaattgcca tgtagatgtt catacaatcg tcaaatcatg aaggctggaa
     1861 aagccctcca agatccccaa gaccaacccc aacccaccca ccgtgcccac tggccatgtc
     1921 cctcagtgcc acatccccac agttcttcat cacctccagg gacggtgacc cccccacctc
     1981 cgtgggcagc tgtgccactg cagcaccgct ctttggagaa ggtaaatctt gctaaatcca
     2041 gcccgaccct cccctggcac aacgtaaggc cattatctct catccaactc caggacggag
     2101 tcagtgagga tggggctcta ggaataaaat atctttattt tcattacatc tgtgtgttgg
     2161 ttttttgtgt ggtcgacatt gattattgac tagatcatcg cgtgaggctc cggtgcccgt
     2221 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2281 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2341 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2401 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2461 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacgcccctg
     2521 gctgcagtac gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag
     2581 gccttgcgct taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg
     2641 gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc
     2701 tctagccatt taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct
     2761 tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga
     2821 cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc
     2881 gagaatcgga cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc
     2941 gccgtgtatc gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc
     3001 ggaaagatgg ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc
     3061 gggagagcgg gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt
     3121 cgcttcatgt gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag
     3181 cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca
     3241 cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga
     3301 atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag
     3361 tttttttctt ccatttcagg tgtcgtgagg aattctgcag tcgacggtac cgcgggcccg
     3421 ggatccaccg gtcgccacca tggtgaaaac tgtgaaagtg aatataacaa cccctgatgg
     3481 gccagtctac gacgctgata tcgagatggt gtccgtgcgg gccgagagtg gtgatctcgg
     3541 catcctcccc ggtcacattc ccacaaaggc cccactgaag atcggagctg tgcggctgaa
     3601 gaaggacggc caaaccgaga tggtcgcagt ctcaggcggc actgttgaag tgcggcctga
     3661 ccacgttacc attaatgctc aagccgctga aacagccgaa ggaatcgaca aagagagagc
     3721 agaagccgca agacagaggg cccaggagcg gctgaactct caaacccgtt acaacagcca
     3781 caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg
     3841 ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat
     3901 cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccaagctgag
     3961 caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg
     4021 gatcactctc ggcatggacg agctgtacaa gggtggcacc ggagggagca tggtgagcaa
     4081 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4141 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4201 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4261 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4321 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4381 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4441 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4501 caacctgggt gacatcgact tcaaacgggc cgaactggcg ttaaaacgcg ccatgaaccg
     4561 tttgagcgtt gcggaaatga agtgagcggc cgctcgataa gcttgatcga tgatctagag
     4621 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4681 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4741 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4801 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4861 tggcttctga ggcggaaaga acctgcagct tttcccaagg cagtctggag catgcgcttt
     4921 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     4981 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     5041 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     5101 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     5161 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     5221 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     5281 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     5341 gctgttctcc tcttcctcat ctccgggcct ttcgacctgt ggccacaacc atgaccgagt
     5401 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     5461 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     5521 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     5581 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     5641 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     5701 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     5761 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5821 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5881 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5941 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     6001 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgatg atctagagct
     6061 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     6121 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     6181 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     6241 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     6301 gctgcagccc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     6361 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     6421 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     6481 acctgtcgtg ccagcggatc cctagaggga cagccccccc ccaaagcccc cagggatgta
     6541 attacgtccc tcccccgcta gggggcagca gcgagccgcc cggggctccg ctccggtccg
     6601 gcgctccccc cgcatccccg agccggcagc gtgcggggac agcccgggca cggggaaggt
     6661 ggcacgggat cgctttcctc tgaacgcttc tcgctgctct ttgagcctgc agacacctgg
     6721 ggggatacgg ggaaaaagct ttaggctgaa agagagattt agaatgacag aatcatagaa
     6781 cggcctgggt tgcaaaggag cacagtgctc atccagatcc aaccccctgc tatgtgcagg
     6841 gtcatcaacc agcagcccag gctgcccaga gccacatcca gcctggcctt gaatgcctgc
     6901 agggatgggg catccacagc ctccttgggc aacctgttca gtgcgtcacc accctctggg
     6961 ggaaaaactg cctcctcata tccaacccaa acctcccctg tctcagtgta aagccattcc
     7021 cccttgtcct atcaaggggg agtttgctgt gacattgttg gtctggggtg acacatgttt
     7081 gccaattcag tgcatcacgg agaggcagat cttggggata aggaagtgca ggacagcatg
     7141 gacgtgggac atgcaggtgt tgagggctct gggacactct ccaagtcaca gcgttcagaa
     7201 cagccttaag gataagaaga taggatagaa ggacaaagag caagttaaaa cccagcatgg
     7261 agaggagcac aaaaaggcca cagacactgc tggtccctgt gtctgagcct gcatgtttga
     7321 tggtgtctgg atgcaagcag aaggggtgga agagcttgcc tggagagata cagctgggtc
     7381 agtaggactg ggacaggcag ctggagaatt gccatgtaga tgttcataca atcgtcaaat
     7441 catgaaggct ggaaaagccc tccaagatcc ccaagaccaa ccccaaccca cccaccgtgc
     7501 ccactggcca tgtccctcag tgccacatcc ccacagttct tcatcacctc cagggacggt
     7561 gaccccccca cctccgtggg cagctgtgcc actgcagcac cgctctttgg agaaggtaaa
     7621 tcttgctaaa tccagcccga ccctcccctg gcacaacgta aggccattat ctctcatcca
     7681 actccaggac ggagtcagtg aggatggggc tctagccgga ccgacgcgtc tcgataggga
     7741 taacagggta attgatccaa tactcaagta caattttaat ggagtacttt tttactttta
     7801 ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt tccctaagta
     7861 cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgga gctccagctt
     7921 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
     7981 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     8041 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     8101 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     8161 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     8221 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     8281 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     8341 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     8401 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     8461 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     8521 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     8581 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     8641 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     8701 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     8761 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     8821 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     8881 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     8941 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     9001 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     9061 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     9121 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     9181 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     9241 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     9301 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     9361 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     9421 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     9481 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     9541 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     9601 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     9661 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     9721 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     9781 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     9841 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     9901 caccagcgtt tctgggtgag caaaaacggg aaggcaaaat gccgcaaaaa agggaataag
     9961 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    10021 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    10081 aggggttccg cgcacatttc cccgaaaagt gccac
//
