LOCUS       sequence-265787-        8953 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATP measurement, nucleus, lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8953)
  AUTHORS   Yaginuma H,  Okada Y
  TITLE     Live cell imaging of metabolic heterogeneity by quantitative 
            fluorescent ATP indicator protein, QUEEN-37C
  JOURNAL   bioRxiv 2021.10.08.463131
REFERENCE   2  (bases 1 to 8953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.10.08.463131"
COMMENT     Sequence Label: NLS-QUE37C/pLBS CAG
FEATURES             Location/Qualifiers
     source          1..8953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        661..887
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             888..1068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1115..1240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1733..1966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2151..2195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2462..2578
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2701..3004
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3006..3281
                     /label=chicken beta-actin promoter
     intron          3282..4298
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     4222..4244
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     4306..4325
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4354..4370
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     4355..4371
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4401..4421
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      4434..4443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4800..5051
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     4988..5009
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5115..5136)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5376..5395)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     misc_feature    5622..6210
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5675..5695)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6093..6104)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6301..6534
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6606..6687
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6643..6662)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6697..6716
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(6765..6783)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6804..6820)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6804..6820)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6817..6839)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6828..6844
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6852..6858,6859..6876,6877..6882))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6897..6918
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7035..7052)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7206..7794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7286..7305)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7965..8756,8757..8825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8588..8607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8826..8930)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccacgcg
      661 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
      721 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      781 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      841 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      901 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      961 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     1021 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     1081 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     1141 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     1201 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     1261 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     1321 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     1381 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     1441 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     1501 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     1561 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     1621 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1681 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1741 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1801 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1861 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1921 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1981 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     2041 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     2101 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     2161 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     2221 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     2281 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     2341 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     2401 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
     2461 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
     2521 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttgg
     2581 gcccttccaa ccttccaacc ggtaccggtt ggaaggttgg aagggccctt ccaaccttcc
     2641 aaccggtacc tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     2701 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     2761 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     2821 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     2881 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     2941 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     3001 catggtcgag gtgagcccca cgttctgctt cactctcccc atctcccccc cctccccacc
     3061 cccaattttg tatttattta ttttttaatt attttgtgca gcgatggggg cggggggggg
     3121 gggggggcgc gccrggsggg gsgggggggg gcgggggggg gggcggggcg rggcggagag
     3181 gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt tccttttatg gcgaggcggc
     3241 ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc gggagtcgct gcgcgctgcc
     3301 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
     3361 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagcgct
     3421 tggtttaatg acggcttgtt tcttttctgt ggctgcgtga aagccttgag gggctccggg
     3481 agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     3541 gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg ctgcgggcgc ggcgcggggc
     3601 tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
     3661 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
     3721 ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca cccccctccc cgagttgctg
     3781 agcacggccc ggcttcgggt gcggggctcc gtacggggcg tggcgcgggg ctcgccgtgc
     3841 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
     3901 agggctcggg ggaggggcgc ggcggccccc ggagcgccgg cggctgtcga ggcgcggcga
     3961 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
     4021 aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac cccctctagc gggcgcgggg
     4081 cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga gggccttcgt gcgtcgccgc
     4141 gccgccgtcc ccttctccct ctccagcctc ggggctgtcc gcggggggac ggctgccttc
     4201 gggggggacg gggcagggcg gggttcggct tctggcgtgt gaccggcggc tctagagcct
     4261 ctgctaacca tgttcatgcc ttcttctttt tcctacagct cctgggcaac gtgctggtta
     4321 ttgtgctgtc tcatcatttt ggcaaaggat tcctcgaggt cgacggtatc gataagcttg
     4381 atatcgaatt caccatggcg ccgaaaaaaa aacgcaaagt ggatccaccg gtcgccacca
     4441 tggtgaaaac tgtgaaagtg aatataacaa cccctgatgg gccagtctac gacgctgata
     4501 tcgagatggt gtccgtgcgg gccgagagtg gtgatctcgg catcctcccc ggtcacattc
     4561 ccacaaaggc cccactgaag atcggagctg tgcggctgaa gaaggacggc caaacagagg
     4621 atgtcgcagt ctcaggcggc actgttgaag tgcggcctga ccacgttacc attaatgctc
     4681 aagccgctga acgggcggag gacattgacg tcctccgcgc caaagcggcg aaagagcggg
     4741 cggagcgccg cctgcaaagc cagacccgtt acaacagcca caacgtctat atcatggccg
     4801 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     4861 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     4921 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     4981 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     5041 agctgtacaa gggtggcacc ggagggagca tggtgagcaa gggcgaggag ctgttcaccg
     5101 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
     5161 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     5221 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
     5281 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     5341 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     5401 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     5461 tcaaggagga cggcaacatc ctggggcaca agctggagta caacctgggt gacatcgact
     5521 tcaaacgggc cgaactggcg ttaaaacgcg ccatgaaccg tttgagcgtt gcggaagata
     5581 agtgagcggc cgcgactcta gatcactagt ctcgactcga caatcaacct ctggattaca
     5641 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5701 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5761 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5821 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5881 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5941 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     6001 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     6061 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     6121 cccgcggcct gctgccggct ctgcggcctc ttccgcatct tcgccttcgc cctcagacga
     6181 gtcggatctc cctttgggcc gcctccccgc ctggaattaa ttcgagctcg gtacctttaa
     6241 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6301 tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt actgggtctc
     6361 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     6421 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     6481 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     6541 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     6601 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6661 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6721 tatctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     6781 atttcgagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     6841 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     6901 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     6961 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7021 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7081 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7141 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7201 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     7261 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     7321 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     7381 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     7441 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     7501 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     7561 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     7621 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     7681 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     7741 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8101 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8941 cgaaaagtgc cac
//
