LOCUS       sequence-265796-        5781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse Fos in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5781)
  AUTHORS   Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark 
            NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, 
            Lemischka IR, Moore K
  TITLE     Induction of a hemogenic program in mouse fibroblasts.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 
            10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.
  PUBMED    23770078
REFERENCE   2  (bases 1 to 5781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.05.024"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "205-18"
COMMENT     Sequence Label: pMXs-Fos
FEATURES             Location/Qualifiers
     source          1..5781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    642..999
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1058..1474
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1404..1426
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1442..1458
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1484..1858
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1796..1812
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             3250..3719
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(3849..3866)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4020..4608)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4100..4119)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4772..5563,5564..5632))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5395..5414
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5633..5737)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             join(5767..5781,1..579)
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
       61 actgagaata gaaaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
      121 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
      181 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      241 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
      301 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
      361 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
      421 acccctcact cggcgcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
      481 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
      541 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
      601 gagacccctg cccagggacc accgacccac caccgggagg taagctggcc agcaacttat
      661 ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag
      721 ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc
      781 ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga
      841 gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc ccctaatagg agggatatgt
      901 ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc
      961 ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc tgtgttgtct
     1021 ctgtctgact gtgtttctgt atttgtctga aaattagggc cagactgtta ccactccctt
     1081 aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc agtcggtaga
     1141 tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg
     1201 atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga tcaaggtctt
     1261 ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct gggaagcctt
     1321 ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc cgcctcctct
     1381 tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc ctcgatcctc
     1441 cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg agatcttata
     1501 tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag ttactaacag
     1561 cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag tctggagacc
     1621 tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc cttaccgagt
     1681 cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc gctggaaagg
     1741 accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca tcgcagcttg
     1801 gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct ctagactgcc
     1861 ggatctagct agttaattaa ggatctcgag ctcaagcttc gaattcatga tgttctcggg
     1921 tttcaacgcc gactacgagg cgtcatcctc ccgctgcagt agcgcctccc cggccgggga
     1981 cagcctttcc tactaccatt ccccagccga ctccttctcc agcatgggct ctcctgtcaa
     2041 cacacaggac ttttgcgcag atctgtccgt ctctagtgcc aactttatcc ccacggtgac
     2101 agccatctcc accagcccag acctgcagtg gctggtgcag cccactctgg tctcctccgt
     2161 ggccccatcg cagaccagag cgccccatcc ttacggactc cccacccagt ctgctggggc
     2221 ttacgccaga gcgggaatgg tgaagaccgt gtcaggaggc agagcgcaga gcatcggcag
     2281 aaggggcaaa gtagagcagc tatctcctga agaggaagag aaacggagaa tccgaaggga
     2341 acggaataag atggctgcag ccaagtgccg gaatcggagg agggagctga cagatacact
     2401 ccaagcggag acagatcaac ttgaagatga gaagtctgcg ttgcagactg agattgccaa
     2461 tctgctgaaa gagaaggaaa aactggagtt tattttggca gcccaccgac ctgcctgcaa
     2521 gatccccgat gaccttggct tcccagagga gatgtctgtg gcctccctgg atttgactgg
     2581 aggtctgcct gaggcttcca ccccagagtc tgaggaggcc ttcaccctgc cccttctcaa
     2641 cgaccctgag cccaagccat ccttggagcc agtcaagagc atcagcaacg tggagctgaa
     2701 ggcagaaccc tttgatgact tcttgtttcc ggcatcatct aggcccagtg gctcagagac
     2761 ctcccgctct gtgccagatg tggacctgtc cggttccttc tatgcagcag actgggagcc
     2821 tctgcacagc aattccttgg ggatggggcc catggtcaca gagctggagc ccctgtgtac
     2881 tcccgtggtc acctgtactc cgggctgcac tacttacacg tcttcctttg tcttcaccta
     2941 ccctgaagct gactccttcc caagctgtgc cgctgcccac cgaaagggca gcagcagcaa
     3001 cgagccctcc tccgactccc tgagctcacc cacgctgctg gccctgtgag gatccgttaa
     3061 cgcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa
     3121 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     3181 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     3241 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     3301 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3361 gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc agccctcagc
     3421 agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg
     3481 ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc
     3541 gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg attgactgag
     3601 tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac ttgtggtctc
     3661 gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg ggtctttcac
     3721 atgcagcatg tatcaaaatt aatttggttt tttttcttaa gtatttacat taaatggcca
     3781 tagttgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3841 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3901 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3961 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4021 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4081 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4141 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4201 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4261 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4321 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4381 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4441 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4501 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4561 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4621 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4681 atgagattat caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct
     4741 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     4801 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     4861 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     4921 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     4981 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5041 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5101 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5161 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5221 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5281 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5341 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5401 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5461 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     5521 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     5581 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     5641 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     5701 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     5761 ctgcataatg aaagacccca c
//
