LOCUS       sequence-265802-        2634 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pFREE019Env
FEATURES             Location/Qualifiers
     source          1..2634
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagagtga aggggacaca gatgagttgg ccaaacttgt ggaaatgggg gactttgatc
       61 cttgggttgg tgataatttg tagtgcctca gacaacttgt gggttacagt ttattatggg
      121 gtccctgtat ggaaagatgc agataccacc ctattttgtg catcagatgc caaagcacaa
      181 gagacagaag cgcacaatgt ctgggccaca catgcctgtg tacccacaga ccccaaccca
      241 caagaaatat acatggaaaa tgtaacagaa aattttaaca tgtggaaaaa taacatggta
      301 gagcagatgc aggaggatgt aatcagttta tgggatcaaa gtctacagcc atgtgtaaag
      361 ctaactcctc tctgcgtaac tttaaattgt actgatgcta aagtgatcct tgacacaaac
      421 acaacccaag ccccaagcac aaacacaacc accacaaaca caaccactgt ccctagagta
      481 aacacaacag acgaagtaaa aaactgttct tttaatacaa ccacagtaat aagagataag
      541 aagcagaagg tccatgcact tttttatagg cttgatatag taccaataga taacagtggt
      601 caaatggata acagtagtag tagggagtat aggttaataa attgtaatac ttcagttatt
      661 aagcaggctt gtccaaaaat atcctttgat ccaattccta tacattattg tactcctgct
      721 ggttatgtga ttttaaagtg taatgataag aagttcaatg ggacagggcc atgtagaaat
      781 gtcagctcag tacaatgcac acatggaatt aagccagtgg tatcaactca attgctgtta
      841 aatggcagtc tagctgaaga agatataata atcagatctg aaaatctcac aaacaatgca
      901 aaaaccataa tagtgcacct taataaatct gtagaaatca attgtaccag accctccaac
      961 aatacaagaa aaggtacaac tataggacca ggacaagtat tctatagaac gggagaaata
     1021 ataggagata taagacaagc acattgtgag attaatggaa caaaatggaa ggaagtgtta
     1081 acacaggtag ctaaagaatt acaaaagcac tttaacaata agccaataaa atttcaacca
     1141 ccctcaggag gagatctaga aattacaact catcatttca attgtagagg ggaatttttc
     1201 tattgcaata catcaaacct gtttaatagt acttataata acactgagtt aactgtaggt
     1261 aataatactg ataatgatac tatcacgctt ccatgcagga taaagcaaat tataaacatg
     1321 tggcagggag caggacaagc aatgtatgct cctcccatca gtggaaaaat taattgtata
     1381 tcaaatatta caggaatatt attgacaaga gatggtggta ataatgcaac aaatgaagag
     1441 atcttcagac ctgaaggagg aaatataaag gacaattgga gaagtgaatt atataaatat
     1501 aaagtagtac aaattgaacc actaggaata gcacccacca gggcaaagag aagagtggtg
     1561 gagagagaaa aaagagcagt gggaatagga gctatgatct ttgggttctt aggagcagcc
     1621 ggaagcacta tgggcgcagc gtcaataacg ctgacggtac aggccagaca attattgtct
     1681 ggtatagtgc aacagcaaaa caatttgctg agggctatag aggcgcagca gcatatgttg
     1741 caactcacag tctggggcat taaacagctc caggcaagag tcctggctgt ggaaagatac
     1801 ctaaaggatc aaaagttcct aggactttgg ggctgctctg gaaaaatcat ctgcaccact
     1861 gctgtgccct ggaactccac ttggagtaat aaatcttttg atgagatttg gaacaacatg
     1921 acatgggcag aatgggaaag agaaattagc aattacacaa accaaatata tgacatactt
     1981 acagaatcac agaaccagca ggacaggaat gaaaaggatt tgttagaatt agataaatgg
     2041 acaagtctgt ggagttggtt tgacataaca aattggctgt ggtatataaa aatatttata
     2101 atgatagtag gaggtttaat aggtttaaga ataatttttg ctgtgctttc tatagtaaat
     2161 agagttaggc agggttactc acctttgtct ttccagatcc ctacccatca gcagagggaa
     2221 ccagacaggc ccgaaagaac cgaagaagga ggtggcgagc aaggcagaga cagatccgtg
     2281 agattagtga gcgggttctt agcgcttgcg tgggacgacc tacggagcct gtgcctcttc
     2341 agctaccacc tcttgagaaa cttcatctcg attgcagcga ggactgtgga acttctggga
     2401 cgcagcagcc tcaagggact gagacggggg tgggaaggtc ttaaatatct agggaatctt
     2461 ctgttatatt ggggccagga actaaaaatt agtgctatct ctttgcttga tgccacagca
     2521 ataacaatag cggggtggac agataggatt atagaactag cacaaggagt tgggagagcc
     2581 attctacata tacctaggag aatcagacag gggttggaaa gggctttgct ataa
//
