LOCUS       sequence-265823-        2535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express HIV Env which is isolated a from IRB approved HIV infected 
            patient samples from Singapore and cloned into expression vector 
            (pcDNA 3.1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Joshi A, Cox EK, Sedano MJ, Punke EB, Lee RT, Maurer-Stroh S, Kaur 
            P, Ng OT, Garg H
  TITLE     HIV-1 subtype CRF01_AE and B differ in utilization of low levels of 
            CCR5, Maraviroc susceptibility and potential N-glycosylation sites.
  JOURNAL   Virology. 2017 Dec;512:222-233. doi: 10.1016/j.virol.2017.09.026. 
            Epub 2017 Oct 9.
  PUBMED    29020646
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Virology.";
            date: "2017-12"; volume: "512:222-233. doi"; pages: " 
            10.1016/j.virol.2017.09.026. Epub 2017 Oct 9"
COMMENT     Sequence Label: pHM024Env
FEATURES             Location/Qualifiers
     source          1..2535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1509..1742
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2265..2291
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 atgagagtga gggggatcag gaggaattac ttatggaaat gggggaaatg gggcatctta
       61 ttcctgggga tattgatgat ctgtagcgct gcagacaatt tgtgggtcac agtctattat
      121 ggggtacctg tgtggagaga tgcaaacacc actctatttt gtgcatcaga tgctaaagca
      181 tatgaaagag aagtacataa tgtttgggcc acacatgcct gtgtacccac agaccccaac
      241 ccacaagaat tggtattgga aaatgtgaca gaagagttta atatgtggaa aaataacatg
      301 gtagaacaga tgcatgagga cataatcagt ttatgggatc aaagcctaaa accatgtgta
      361 aaattaaccc cactctgtgt cactttaaat tgcactgacg ctaccagtgg taataatacc
      421 agtggtaata taacggggga gggaggaatg aaaaactgct cttttaatgt caccacagac
      481 ataaaagata agagaataga aaaagcattg ttttataaag atgatttagt acaaacaggg
      541 aagaataata ctatttatag gttgatgagc tgtaacacct cagtcattac ccaagcctgt
      601 ccaaaggtat cctttgagcc aattcccata cattattgta ccccggctgg ttatgcgatt
      661 cttaagtgta ataataagac atttaatgga ttagggcgat gtacaaatgt cagcacagta
      721 ctatgtacac atggaattag gccagtagta tcaactcaac tgatgttaaa tggcagttta
      781 gcagaagaag aggtagtaat tagatctgaa aatttcacaa acaatgctaa aaccataata
      841 gtacagctga aagaagctgt aaaaattaat tgcacaagac ccaacaacaa tacaagaaaa
      901 agtatacata tggggcaagg gaaagcatgg tatacaacag gagagataat aggagacata
      961 agacaagcat attgtacaat taacaaaaca aaatggaatg acactttaaa acagatagct
     1021 gaaaaattaa gaagacaatt tgggaataaa acaatagtct ttaatcagcc ctcaggaggg
     1081 gacccagaga ttgtaatgca cagttttaat tgtggagggg aatttttcta ctgtaataca
     1141 tcacaactat ttaatagtac ttgggatgga aatagtacta gggagaatag cactggacag
     1201 ttattcacac tcccatgcag aataaaacaa attataaaca tgtggcagaa agtaggaaaa
     1261 gcaatgtatg cccctcccat cgcgggccca attaactgtt tatcaaacat tacagggctg
     1321 ttattaacaa gagatggtgg tggtaataat acaaataatg agaccttcag accaggagga
     1381 ggagatatga gggacaattg gagaagtgaa ctatacaaat ataaagtagt aagagttgaa
     1441 ccattaggac tggcacccac cagggcaaag agaagagtgg tgcagagaga aaaaagagca
     1501 gtgggactag gagcattgtt cattgggttc ttgggagcag caggaagcac tatgggcgca
     1561 gcgtcagtga cgctgacggt acaggccaga ctattattgt ctggtatagt gcaacagcag
     1621 aacaatctgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1681 atcaagcagc tccaggcaag agtcctggct gtggaaagat acctaaggga tcaacagctc
     1741 ctaggaatgt ggggttgctc tggaaaactc atttgcccca cctctgtgcc ttggaatgct
     1801 agttggagca ataaatctct ggaagatatt tggaataaca tgacctggag gcaatgggag
     1861 acagaaattg acaattacac aggcttaata tacaccttac ttacagaatc gcagaaccaa
     1921 caggaaaaga atgaacaaga attattagaa ttggataaat gggcaaattt atggaattgg
     1981 tttgacatat caaactggct gtggtatata agaatattca taatgatagt aggaggcttg
     2041 ataagtttaa gaatagtttt tactgtactt tctataataa atagagttag gcagggatac
     2101 tcaccattgt cgttgcagac ccacctccca gctcagaggg gacccgacag gccagaagga
     2161 atcgaagaag aaggtggaga gagagacaga gacagatccg gacgattagt gggtggattc
     2221 ttgacactta tctgggtcga cctgaggagc ctgttcctct acagctacca ccgcttgaga
     2281 gacttactct tgattgtaac gaggattgtg gaacttctgg gacgcagggg gtgggaactc
     2341 ctcaagtact gggggaatct cctacagtat tggggtcagg aactaaagaa tagtgctatt
     2401 agcttgctca acaccacagc catagcagta gctgagggga cagataggct tatagaatta
     2461 gtgcaattaa cttttagagc tattcttcac atacctagaa gaataagaca gggcttagaa
     2521 aggactttgc tataa
//
