LOCUS       sequence-265860-        9418 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral Gateway destination vector, Expression, CMV/TO promoter, 
            Hygro.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Campeau E, Ruhl VE, Rodier F, Smith CL, Rahmberg BL, Fuss JO, 
            Campisi J, Yaswen P, Cooper PK, Kaufman PD.
  TITLE     A versatile viral system for expression and depletion of proteins in
            mammalian cells.
  JOURNAL   PLoS One. 2009 Aug 6;4(8):e6529.
  PUBMED    19657394
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-08-6"; volume: "4(8)"; pages: "e6529"
COMMENT     Sequence Label: pQCXIH CMV/TO DEST (w382-1)
FEATURES             Location/Qualifiers
     source          1..9418
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        2..381
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..585
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             586..761
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    824..1023
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1224..1640
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1570..1592
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1608..1624
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        1985..2188
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    2190..2208
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2211..2229
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2270..2394
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(2419..2424,2425..2442,2443..2449)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             2503..3162
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2569..2588)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             3504..3809
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3688..3707
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3850..3974)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    4130..4592
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4186..4203)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4413..4432
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4631..5650
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             4631..5650
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     LTR             6197..6622
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(6725..6747)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6883..6903)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6888..7217
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7068..7203
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7239..7258
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7339..7356)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7510..8098)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7590..7609)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8269..9060,9061..9129))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8892..8911
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9130..9234)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9302..9320
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc
       61 ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc
      121 aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg
      181 actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat
      241 caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc
      301 tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta catctacgta
      361 ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag
      421 cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt
      481 tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa
      541 atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctcaata aaagagccca
      601 caacccctca ctcggcgcgc cagtcttccg atagactgcg tcgcccgggt acccgtattc
      661 ccaataaagc ctcttgctgt ttgcatccga atcgtggtct cgctgttcct tgggagggtc
      721 tcctctgagt gattgactac ccacgacggg ggtctttcat ttgggggctc gtccgggatt
      781 tggagacccc tgcccaggga ccaccgaccc accaccggga ggtaagctgg ccagcaactt
      841 atctgtgtct gtccgattgt ctagtgtcta tgtttgatgt tatgcgcctg cgtctgtact
      901 agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag ttctgaacac
      961 ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt ggcccgacct
     1021 gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg gtaggagacg
     1081 agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg aaccgaagcc
     1141 gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt
     1201 ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt ttgaccttag
     1261 gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc aagaagagac
     1321 gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg ccgcgagacg
     1381 gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca cctggcccgc
     1441 atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct tttgaccccc
     1501 ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct ccatccgccc
     1561 cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt tatccagccc
     1621 tcactccttc tctaggcgcc ggaattgaag atctggggga tcgataagct tgggagttcc
     1681 gcttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     1741 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     1801 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     1861 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     1921 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     1981 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     2041 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     2101 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     2161 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
     2221 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
     2281 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     2341 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     2401 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     2461 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     2521 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     2581 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     2641 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     2701 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     2761 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     2821 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     2881 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     2941 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     3001 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     3061 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     3121 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     3181 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     3241 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     3301 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     3361 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     3421 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     3481 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     3541 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     3601 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     3661 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     3721 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     3781 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     3841 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     3901 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3961 ttgtacaaag tggttgatat ccagcacagt ggcgaccggt aggcctcgta cgcttaatta
     4021 acggaccctc ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg
     4081 cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga
     4141 aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa
     4201 tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa
     4261 caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct
     4321 gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg
     4381 ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg
     4441 ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtgca
     4501 catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg
     4561 acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccgtac caaagatgga
     4621 tagatccgga aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
     4681 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
     4741 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
     4801 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     4861 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     4921 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
     4981 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     5041 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     5101 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     5161 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     5221 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     5281 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     5341 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     5401 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     5461 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     5521 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     5581 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     5641 aaaggaatag agtagatgcc gaccgaacaa gagctgattt cgagaacgcc tcagccagca
     5701 actcgcgcga gcctagcaag gcaaatgcga gagaacggcc ttacgcttgg tggcacagtt
     5761 ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg gagggccggt cgcagtgatt
     5821 caggcccttc tggattgtgt tggtccccag ggcacgattg tcatgcccac gcactcgggt
     5881 gatctgactg atcccgcaga ttggagatcg ccgcccgtgc ctgccgattg ggtgcagatc
     5941 tctatcgagg gctgcagcgc tgcagaggcc gagtgcagaa ctgctccaaa gggacctcaa
     6001 ggctttccga gggacactag gctgactcca tcgagccagt gtagagataa gcttatcgat
     6061 tagtccaatt tgttaaagac aggatatcag tggtccaggc tctagttttg actcaacaat
     6121 atcaccagct gaagcctata gagtacgagc catagataaa ataaaagatt ttatttagtc
     6181 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa
     6241 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     6301 caggaacaga tggaacaggg tcgaccctag agaaccatca gatgtttcca gggtgcccca
     6361 aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct tctcgcttct
     6421 gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc actcggggcg
     6481 ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac cctcttgcag
     6541 ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt gattgactac
     6601 ccgtcagcgg gggtctttca tttgggggct cgtccgggat cgggagaccc ctgcccaggg
     6661 accaccgacc caccaccggg aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg
     6721 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6781 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     6841 tgacccagtc acgtagcgat agcggagtgt agatccggct gtggaatgtg tgtcagttag
     6901 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6961 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     7021 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     7081 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     7141 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     7201 gcctaggctt ttgcaaaaag cttactggct taactatgcg gcatcagagc agattgtact
     7261 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     7321 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     7381 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     7441 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7501 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7561 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7621 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7681 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7741 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7801 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7861 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7921 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7981 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8041 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8101 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8161 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8221 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8281 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     8341 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     8401 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     8461 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8521 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8581 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8641 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8701 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8761 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8821 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8881 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8941 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9001 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9061 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9121 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9181 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9241 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     9301 aaataggcgt atcacgaggc cctttcgtct tcaagaatta gcttggccat tgcatacgtt
     9361 gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac cgccatgt
//
