LOCUS       sequence-265874-        7533 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length BMR1_03g00785 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7533)
  AUTHORS   Elton CM, Rodriguez M, Ben Mamoun C, Lobo CA, Wright GJ
  TITLE     A library of recombinant Babesia microti cell surface and secreted 
            proteins for diagnostics discovery and reverse vaccinology.
  JOURNAL   Int J Parasitol. 2019 Feb;49(2):115-125. doi: 
            10.1016/j.ijpara.2018.10.003. Epub 2018 Oct 24.
  PUBMED    30367868
REFERENCE   2  (bases 1 to 7533)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ijpara.2018.10.003"; journalName: "Int J Parasitol"; 
            date: "2019-02"; volume: "49"; issue: "2"; pages: "115-125"
COMMENT     Sequence Label: BMR1_03g00785-bio-His
FEATURES             Location/Qualifiers
     source          1..7533
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        387..590
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     541..561
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     587..611
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1092..1111
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1134..1143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2697..2714
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2766..2821
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2820..2839)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5057..5075)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5143..5247
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5248..5316,5317..6108)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5466..5485)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6279..6867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6768..6787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7021..7038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7155..7176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7191..7196,7197..7214,7215..7221)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7229..7245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7234..7256
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7253..7269
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7253..7269
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
      601 accgtcagat cctcactctc ttccgcatcg ctgtctgcga gggccagctg ttgggctcgc
      661 ggttgaggac aaactcttcg cggtctttcc agtactcttg gatcggaaac ccgtcggcct
      721 ccgaacggta ctccgccacc gagggacctg agcgagtccg catcgaccgg atcggaaaac
      781 ctctcgagaa aggcgtctaa ccagtcacag tcgcaaggta ggctgagcac cgtggcgggc
      841 ggcagcgggt ggcggtcggg gttgtttctg gcggaggtgc tgctgatgat gtaattaaag
      901 taggcggtct tgagacggcg gatggtcgag gtgaggtgtg gcaggcttga gatccagctg
      961 ttggggtgag tactccctct caaaagcggg cattacttct gcgctaagat tgtcagtttc
     1021 caaaaacgag gaggatttga tattcacctg gcccgatctg gccatacact tgagtgacaa
     1081 tgacatccac tttgcctttc tctccacagg tgtccactcc caggtccaag tttgccacca
     1141 tggagtttca gacccaggta ctcatgtccc tgctgctctg catgtctggt gcggccgccg
     1201 ctggtggaag cggaggaaat ggtggcaacg gcggaggcca ccaggaacag aacaacgcca
     1261 acgacgccag caaccctgcc ggcgctggcg gccagcccaa caacgaggcc aagaaaaagg
     1321 ccgtgaagct ggacctggac ctgatgaagg aaacaaagaa cgtctgcacc accgtgaaca
     1381 ccaagctcgt gggcaaggcc aagagcaagc tgaacaagct ggaaggcgag agccacaaag
     1441 aatacgtggc cgaaaagacc aaagagatcg acgagaagaa caagaagttc aacgagaacc
     1501 tggtcaagat cgagaagcgg aagaaaatca aggtgcccgc cgacacaggc gccgaggtgg
     1561 acgctgtgga tgatggcgtg gcaggcgccc tgagcgacct gagcagcgac atcagcgcca
     1621 tcaagaccct gaccgacgac gtgtccgaga aggtgtccga aaacctgaag gacgacgagg
     1681 ccagcgccac cgagcacacc gacatcaaag agaaggccac cctgctgcag gaaagctgca
     1741 acggcatcgg caccatcctg gacaagctgg ccgagtacct gaacaacgat gccacccaga
     1801 acatcaagaa agagtttgac gagcggaaga agaacctggc cagcctgaaa acaaaggtcg
     1861 agaacaagga cgaggactac gtgacccact tccgggacat ggccaccgag gcccagaacg
     1921 ccgtgggcga agtgaagaaa gccatcgacg ccgtggtggc ccaccggaag gccgagaatc
     1981 tggatgtgga cgacaccctg ttcagcaacc tggccacact gctggacacc atcatcgaga
     2041 catctggcgc gccgtcgacc tccatcacgg cctataagag tgagggggag tcagcggagt
     2101 tctccttccc actcaacctt ggagaggaaa gcctgcaggg agagttgaga tggaaggcag
     2161 agaaggctcc ttcttcccag tcctggatca ccttctccct aaagaaccaa aaggtgtctg
     2221 tgcagaagtc tactagcaac cccaagttcc agctgtccga aacgctccca ctcacccttc
     2281 agatacccca ggtctccctt cagtttgctg gttctggcaa cctgaccctg actctggaca
     2341 gagggatact gtatcaggaa gtgaacctgg tggtgatgaa agtgactcag cccgacagca
     2401 acactttgac ctgtgaggtg atgggaccca cctcacccaa gatgagactg atcttgaagc
     2461 aggagaatca ggaggccagg gtctccaggc aggagaaagt gattcaagtg caggcccctg
     2521 aagcaggggt gtggcaatgt ctactgagtg aaggtgaaga ggtcaagatg gactccaaga
     2581 tccaggtttt atccaaaggg ttgaattccg gcagcctgca ccacatcctg gacgcccaga
     2641 agatgctgtg gaaccacagg gacaggaacc tgcctcctct ggcccctctg ggcccccacc
     2701 accatcacca ccattgagtc tagacacgtg attaattaag gatcccccga cctcgacctc
     2761 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     2821 actcggaagg acatatggga gggcaaatca tttggtcgag atccctcgga gatctctagc
     2881 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
     2941 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
     3001 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
     3061 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
     3121 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
     3181 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
     3241 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
     3301 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
     3361 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
     3421 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
     3481 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
     3541 agtcacaagg gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc
     3601 ctgcttcttc attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag
     3661 gtgtatgtga ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac
     3721 ggggggttca gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg
     3781 caataaatac tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg
     3841 ggtggggaca agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa
     3901 cacataatcc tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca
     3961 agacaggtga accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg
     4021 acagcagcgg actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc
     4081 caatggggcc cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg
     4141 gcacgcgtca gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata
     4201 acttggctga ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact
     4261 aatggcaccc cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt
     4321 gctgcgatct ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt
     4381 ggtcctcata ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat
     4441 atactaccca aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc
     4501 tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc
     4561 tatcctaatt tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc
     4621 tatcctaatc tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc
     4681 tatcctaata gagattaggg tagtatatgc tatcctaatt tatatctggg tagcatatac
     4741 tacccaaata tctggatagc atatgctatc ctaatctata tctgggtagc atatgctatc
     4801 ctaatctata tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc
     4861 ctaatctata tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc
     4921 ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt atatgctatc
     4981 ctaatctgta tccgggtagc atatgctatc ctcatgataa gctgtcaaac atgagaattt
     5041 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5101 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5161 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5221 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5281 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5341 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     5401 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     5461 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     5521 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     5581 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     5641 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     5701 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     5761 ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta
     5821 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     5881 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5941 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6001 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6061 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6121 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6181 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6421 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7021 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     7081 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     7141 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     7201 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     7261 agctatgacc atgattacgc caagctctag ctagaggtcg accaattctc atgtttgaca
     7321 gcttatcatc gcagatccgg gcaacgttgt tgccattgct gcaggcgcag aactggtagg
     7381 tatggaagat ctatacattg aatcaatatt ggcaattagc catattagtc attggttata
     7441 tagcataaat caatattggc tattggccat tgcatacgtt gtatctatat cataatatgt
     7501 acatttatat tggctcatgt ccaatatgac cgc
//
