LOCUS       sequence-265882-         311 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Purpose: synthesis of mRNA encoding a membrane marker. Insert: PH 
            domain of the human phospholipase C delta 1 (PLCD1) fused with the 
            yellow fluorescent protein mCitrine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Guignard L, Fiuza UM, Leggio B, Laussu J, Faure E, Michelin G, 
            Biasuz K, Hufnagel L, Malandain G, Godin C, Lemaire P
  TITLE     Contact area-dependent cell communication and the morphological 
            invariance of ascidian embryogenesis.
  JOURNAL   Science. 2020 Jul 10;369(6500). pii: 369/6500/eaar5663. doi: 
            10.1126/science.aar5663.
  PUBMED    32646972
REFERENCE   2  (bases 1 to 311)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2020-07-10"; volume: "369(6500). pii: 369/6500/eaar5663. 
            doi"; pages: " 10.1126/science.aar5663"
COMMENT     Sequence Label: pGEM-PH-citrine
FEATURES             Location/Qualifiers
     source          1..311
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(74..92)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(75..92)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(110..126)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(110..126)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(123..145)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    134..150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(158..164,165..182,183..188))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    203..224
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 aaaaaaaccc cccccccccc ccccctgcag gcgcgccgcc ggcgatttaa attaattaat
       61 ctagcttgag tattctatag tgtcacctaa atagcttggc gtaatcatgg tcatagctgt
      121 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
      181 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
      241 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
      301 cggggagagg c
//
