LOCUS       sequence-265894-       14858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14858)
  AUTHORS   Yan X, Habedanck R, Nigg EA
  TITLE     A complex of two centrosomal proteins, CAP350 and FOP, cooperates 
            with EB1 in microtubule anchoring.
  JOURNAL   Mol Biol Cell. 2006 Feb;17(2):634-44. Epub 2005 Nov 28.
  PUBMED    16314388
REFERENCE   2  (bases 1 to 14858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2006-02"; volume: "17(2)"; pages: "634-44. Epub 2005 
            Nov 28"
COMMENT     Sequence Label: pcDNA3 CAP350 (Nigg XY97)
FEATURES             Location/Qualifiers
     source          1..14858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     685..704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             839..1633
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(893..912)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1503..1522
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1807..1928
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1844..1863)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1898..1917
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1977..1993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1977..1993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1990..2012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2001..2017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2025..2031,2032..2049,2050..2055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2070..2091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2208..2225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2379..2967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2459..2478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3138..3929,3930..3998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3761..3780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3999..4103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4178..4197)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4369..4748
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4749..4952
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4903..4923
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4997..5016
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4997..5015
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5057..5086
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5090..5119
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5123..5152
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5156..5179
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             11336..11347
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(14583..14600)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    14589..14813
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
      841 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
      901 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
      961 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     1021 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     1081 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     1141 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     1201 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     1261 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     1321 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     1381 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     1441 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     1501 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     1561 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     1621 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     1681 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     1741 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     1801 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     1861 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1921 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     1981 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     2041 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     2101 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     2161 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     2221 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     2281 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     2341 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     2401 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     2461 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     2521 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     2581 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     2641 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     2701 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2761 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     2821 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2881 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2941 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     3001 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     3061 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     3121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     3181 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     3241 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     3301 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     3361 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     3421 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     3481 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     3541 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     3601 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     3661 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     3721 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     3781 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     3841 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     3901 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     3961 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     4021 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     4081 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga
     4141 tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat
     4201 agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca
     4261 aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg
     4321 ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat
     4381 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     4441 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     4501 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     4561 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     4621 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     4681 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     4741 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     4801 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     4861 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     4921 gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa cccactgctt
     4981 actggcttat cgaaattaat acgactcact atagggagac ccaagctggc tagcgtttaa
     5041 acttaagctt accatggagc agaagctgat ctccgaggag gacctgaacg agcagaagct
     5101 gatctccgag gaggacctga acgagcagaa gctgatctcc gaggaggacc tgaacctgga
     5161 ggtgctgttc cagggacccg gggtaccgat gaggagcagc aaatcaaaag aggtgccttt
     5221 accaaatcca aggaactctc aaagcaagga tactgttcaa gcagatataa ccacatcgtg
     5281 ggatgcactt tctcaaacca aggctgctct gagacacatt gaaaataaat tagaagtagc
     5341 ccctacaagt acagctgtgt gtgattctgt catggatacc aagaagtctt ctacaagtgc
     5401 tactcgaaaa ataagtagaa aagatggtag atacctggat gattcttggg ttaatgctcc
     5461 aatctccaaa tccactaaat cacgaaaaga gaaatctcgt agtcctctca gggccaccac
     5521 cctggagagt aatgtgaaga aaaataatcg tgtggaattt cgtgaacctt tggtttctta
     5581 tagggaaatc catggtgcac cttccaattt cagttccagc catctggaat caaagcacgt
     5641 atactgtgta gatgttaatg aagaaaagac tgagagcggt aactggatga taggcagtcg
     5701 agaagaacgg aatatacgga gctgtgattt tgagagctcc caatcatctg tcatcaatga
     5761 tacagttgtt aggtttttaa atgatcgacc agcaattgat gcattgcaaa attctgaatg
     5821 tttgattagg atgggagctt ctatgagaac tgaggaagaa atgcctaaca gaacaaaagg
     5881 aagtgagaat aatttgaagc tttctgtgaa taacatggcc catgatactg atccaaaagc
     5941 gttacgacta actgactctt ctccatcctc tactagtact tctaattccc aaagattaga
     6001 tattctaaag cggcgacaac atgatgtcaa actggaaaaa cttaaggaac ggattagaaa
     6061 acagtgggaa cactcagaag aaacaaatgg ccggggccag aagctgggtc atattgacca
     6121 tccagtaatg gttgttaatg ttgataactc agtaacagca aaagtcagaa aagtggcaac
     6181 agcaccacct gctccagcat ataaaggttt caacccttca gagaccaaga ttcgaacacc
     6241 tgatgggaaa gtgtggcagg aggctgagtt tcaaaacatg agtagagaac tgtatcgaga
     6301 tttagcactt cactttgcag atgatatttc tataaaggag aaacctgctg aaaaaagtaa
     6361 agagaagaaa gtagtcaagc cagtacgaaa agtccaaaaa gtagcacagt tatcaagtac
     6421 agaatgcaga acaggtagta gtcatcttat aagtacatct tcttggcgag atggacaaaa
     6481 attagtaaag aagattctgg gacctgctcc cagaatggag ccaaaagagc aaagaacagc
     6541 atcaagtgac agaggtggaa gagaaagaac tgctaaatct gggggtcaca ttggaagagc
     6601 agaatctgat cccaggttgg acgttttaca tagacatctt caaagaaact cagaacgttc
     6661 gagaagtaaa tctcggtctg aaaataatat aaagaaacta gcttcatctc ttccagataa
     6721 taagcaggag gaaaatactg ccttaaataa ggacttttta cctattgaaa ttcgtggcat
     6781 tcttgatgac ctacagctgg attctacagc tcacactgca aagcaagata ctgtagagtt
     6841 acagaaccag aagtcatcag caccagtaca tgctcctagg agtcacagcc cagtaaaaag
     6901 aaaacctgac aaaataacag ctaatgaaga tccccctgtt atttccaaaa ggcgccacta
     6961 tgacacagat gaggtacgac agtacattgt taggcagcag gaggaaagga agagaaagca
     7021 aaatgaagag aagaaggctc aaaaggaggc tacagaacag aaaaacaaac gattacaaga
     7081 gctctaccgg aagcagaagg aagcctttac taaagtaaaa aatgtccctc cttctgagcc
     7141 atcagcaact aggcgactac aggaaactta ctccaaattg ctactagaaa agaccttgct
     7201 tgaagagcca tctcatcaac atgttacgca ggaaacacag gccaaaccag ggtatcagcc
     7261 atctggagaa tctgacaaag aaaacaaagt acaggaacgt cccccaagtg catcttccag
     7321 tagtgacatg tctctctcag aacctccaca gcctcttgca agaaaagact tgatggaatc
     7381 tacatggatg cagcctgaaa gattgagccc acaagttcac cattctcaac cacagccttt
     7441 tgctggaaca gctggaagtt tactctccca tctcttgagt ttagagcatg taggaatttt
     7501 gcataaggat tttgaatcta ttttaccaac caggaagaat cataatatgg cttcaaggcc
     7561 attaactttt acacctcaac catatgtgac ctcaccagct gcttatacag atgccttgtt
     7621 aaaacctagt gccagccaat ataagagtaa actggatcgt attgaagcct tgaaagcaac
     7681 agctgcttct ttgtccagca gaattgaaag tgaagccaag aaattagctg gggccagcat
     7741 taactatggg tcagcatgga acactgagta tgatgtgcag caggcacctc aagaagatgg
     7801 accttggacc aaggctgtaa ctccacctgt gaaagatgat aatgaagatg ttttctctgc
     7861 cagaattcag aagatgctgg gaagctgtgt atctcatgca acttttgatg atgatcttcc
     7921 tggtgtaggc aatcttagtg aatttaaaaa gcttcctgag atgataagac cacagagtgc
     7981 gatatcaagc tttagagtga gatcccctgg tcccaaacca caagggctac tggcacagtt
     8041 atgtaaaagg cagactgact cttctagctc tgatatgcaa gcctgttctc aagacaaagc
     8101 caaaatatct cttggttcca gcatagattc agtcagtgaa gggcctcttc ttagtgaggg
     8161 gagtctctct gaagtagagg gagaccagga tggacagccc cttttgaaag tagcagaaat
     8221 tttaaaagaa aaggaatttt gtcctggaga aagaaatagt tatgaaccca tcaaagagtt
     8281 tcagaaagaa gctgaaaaat tcttgccact ttttgggcac ataggtggta cacaaagcaa
     8341 aggaccatgg gaagaattgg caaagggaag tccacatagc gtcattaata tttttacaaa
     8401 atcctatcag ttatatggaa aagggtttga agacaagttg gacagaggaa catcaacatc
     8461 acggcctttg aatgccaccg caactcctct aagtggtgtt tcatatgaag atgattttgt
     8521 ctcctctcca gggactggga cttcgacaga aaaaaaatca actcttgaac ctcatagcac
     8581 tttaagccct caggaggacc attctaacag aaagtctgcc tatgatcctt cctctgtgga
     8641 tgttacctcc cagcattcat caggagccca gtctgctgca tcgtctcgtt catctacttc
     8701 ttctaaagga aagaaaggaa aaaaggaaaa gacagaatgg ttggattcat tcactggaaa
     8761 tgttcagaac tcacttcttg atgaggaaaa agcagaacgt ggctcccatc aaggaaagaa
     8821 atctgggacc agcagcaaac tttctgttaa agattttgag cagactcttg atacagatag
     8881 cactttggag gatctttctg gacattctgt gagtgtctca tcagataagg gaagatctca
     8941 gaaaactcca acttctcccc tgtcaccaag ttcccagaaa tcattgcagt ttgacgttgc
     9001 aggaacttct tcagaaagat ctaagtcgtc agtaatgcct ccaactataa caggatttaa
     9061 gcctaatgca cctctcactg atctgaaccc ggcagccagc agaacaacga cagagaacat
     9121 ggctccaata ccaggttcta agcgcttttc tcctgctggc ctccatcatc gtatggcagc
     9181 agaactcagt tatctgaacg ccattgagga gtcggtgcgc caactgtcag atgtagaaag
     9241 agttagaggc atttcacttg ctcagcagga gagtgtgtct ctagctcaga taataaaggc
     9301 acaacagcaa cgccatgaaa gagacttggc cctcttgaaa ctaaaggctg aacaagaggc
     9361 tctggagagt cagagacaat tagaagaaac ccgaaacaaa gcagctcagg tccatgcaga
     9421 atcattacag caggtggttc aatcacaacg ggaagtaact gaagtcctgc aggaagcaac
     9481 gtgtaaaata gcagctcagc agtcagaaac tgctcgcctc accacagacg cagcacgtca
     9541 aatctgtgag atggcagagt tgactagaac tcatatctca gatgctgtcg tggcttcagg
     9601 agctcccctt gcaatactgt atgaccacca acggcagcac cttccagact ttgtgaaaca
     9661 gctgaggacc agaactgaaa cagataggaa aagtccatct gtttcactct ctcagagtaa
     9721 agaagggacc cttgactcaa agcatcagaa gtattctgct tcatatgata gttattctga
     9781 gtcttcagga tacaagaatc atgatagaag aagtagcagt ggtagcagcc gccaagaaag
     9841 tccttcagtt ccatcttgta aggaaaatga gaagaaactt aatggtgaaa agatagagag
     9901 ttccattgat gaacaggttc agactgctgc agatgattct ctacgaagtg atagtgttcc
     9961 atctcttcct gatgaaaaag actcaacgtc tattgcaaca gaatattctc tgaaatttga
    10021 tgaatccatg acagaagatg aaatagaaga acaatcattt cgatcattac taccttcaga
    10081 gagtcaccgc agatttaaca tggaaaagag aagaggtcat catgatgact ctgatgaaga
    10141 agcttctcca gaaaaaacta cactgtctac tgccaaggaa ctgaacatgc cattctcagg
    10201 aggacaagat agcttttcta aatttactat ggagatggtt cgacagtata tgaaagagga
    10261 agaaatgagg gcagctcacc agtcttcact cctgcgtctc cgtgaaaagg ccttgaagga
    10321 gaagactaag gctgaattgg cctggttaga gcatcaaaaa aaacatctac gagacaaagg
    10381 agaggatgat aaaatgcccc cgctccggaa gaaacagcgt ggtttgcttt taaggttgca
    10441 gcaagaaaag gcagaaataa aacgtcttca agaagccaat aaggcagctc ggaaggaaag
    10501 acagctgatt cttaaacagc aggaggagat agaaaagatc cgacagacca ccataaaact
    10561 acaggagaaa ttgaagtctg caggggagag taaattggac tctcatagtg atgatgatac
    10621 aaaggataat aaggcaacca gtcctggtcc aactgacttg gagacccgca gtccttctcc
    10681 catttcaatc tccagcagtg aaactagcag cattatgcag aaactgaaga aaatgagaag
    10741 ccgcatggat gaaaagtttc tgacaaagcg ggagcaaaaa ttaatgcaac ggcgacaaca
    10801 tgcagaggag ctcctcgagt ggaagcgacg tttagatgca gaagaagcag aaattcgtca
    10861 aatggaaaaa caagctttgg ctgcctggga caaagaatta ataaaaccca aaactcctaa
    10921 gaaagaactg gaggaccaga gaacagaaca gaaagaaata gcaagtgaag aggaatctcc
    10981 agtacctctg tactctcatc taaacagtga aagctccatt ccagaagaat taggcagccc
    11041 tgctgttgaa tatgtaccat ccgagtctat aggacaggag cagccaggga gtccagatca
    11101 cagtatactt actgaagaaa tgatttgttc acaggaacta gaatcttcta cctctcctag
    11161 taaacattca cttcccaaaa gctgcacatc tgtgtcaaag caggagtcta gcaaaggaag
    11221 tcataggact ggaggacaat gtcacctgcc tatcaagtcc catcagcact gttatagttg
    11281 gtcagatgag tcattatcta tgacacagtc agaaactaca tctgaccaga gtgatattga
    11341 aggtaggatc agagctctga aggatgagtt gcggaaaaga aaatcagttg tgaaccagct
    11401 gaagaaggaa cagaaaaaaa ggcaaaagga aagactgaaa gcccaagaag ccagtctgat
    11461 caagcagtta gagtcatatg atgaatttat taagaaaact gaagccgagc ttagccaaga
    11521 tttggaaaca tcaccaacag ccaagcctca gattaaaacg ctctcctcag cttctgaaaa
    11581 acccaagatc aaacccctca caccactaca cagatctgaa acggcaaaga attggaaatc
    11641 actaacagag tcagaacgtt ccagaggatc cctggagcct attgctgaac atgttgatgc
    11701 ttcactgtct ggttctgaga gatcagtatc agaaaggtct ttatctgcat atgcaaagag
    11761 agtaaatgaa tgggacagtc gaacagaaga ttttcagacc ccatctccag ttctcagatc
    11821 atcaaggaaa atcagagaag aatctggaga ttctctagaa aatgtacctg cattacatct
    11881 tctcaaagaa ttaaatgcca ctagtagaat tcttgatatg tcagatggca aggttggaga
    11941 atctagtaaa aaatcagaaa taaaagaaat agagtataca aaattgaaga agagtaagat
    12001 tgaagatgcc ttttctaaag aaggtaaatc tgatgtctta ctgaaattag tcctagaaca
    12061 gggagattca tctgaaattc tttcaaagaa agatcttcct ttagattctg aaaatgttca
    12121 gaaagaccta gttggattag ctattgaaaa tctccataaa agtgaggaaa tgttgaaaga
    12181 gagacagtca gatcaagata tgaatcatag tccaaacatc caatcaggaa aagacattca
    12241 cgaacaaaag aacacaaagg aaaaagattt gtcttggtca gaacatcttt ttgctcctaa
    12301 agagatacca tactctgaag attttgaagt gtcttctttc aagaaagaaa tttcagctga
    12361 attgtacaaa gatgattttg aggtgtaatc tttgctgtca ctcaggaaag actctcagtc
    12421 ttgcagagat aagccacagc caatgaggag ctctacaagt ggagccacta gctttggtag
    12481 taatgaggaa atcagtgagt gcctaagtga gaaaagcctt tctatccata gcaatgttca
    12541 ttctgacagg ctgttggaac tcaagtcccc tactgagctg atgaaaagta aggagcgcag
    12601 tgatgtggag catgaacagc aagttactga atccccttcc ttggcttcag ttcctactgc
    12661 agacgagtta tttgatttcc acattggtga tagggtgttg attggaaatg ttcagccagg
    12721 aattcttcga ttcaaaggtg agactagttt tgctaaagga ttttgggccg gagtggagtt
    12781 agataaacct gaaggaaata acaatggaac atatgatggt attgcatatt ttgagtgcaa
    12841 agaaaagcat ggtatttttg ctcctcctca aaaaatatct cacattccag aaaactttga
    12901 tgactatgta gacattaatg aagatgaaga ctgttactca gatgaacgat atcagtgcta
    12961 taatcaagag caaaatgata cagagggtcc aaaagacaga gaaaaggatg tcagtgaata
    13021 tttttatgag aaatccctac ctagtgtgaa tgatatagaa gcctcagtta atagaagtag
    13081 aagccttaaa atagaaacag acaatgtaca ggacatttct ggggtacttg aagcccatgt
    13141 tcaccagcag tcttcagtgg attcacagat ttcttcaaag gaaaacaaag acctcatttc
    13201 tgatgccaca gaaaaggttt ccatcgctgc agaagatgac actttagaca ataccttttc
    13261 cgaagaattg gagaagcaac agcagtttac agaagaggaa gacaacctat atgctgaagc
    13321 ttcagaaaag ctttgtacac cacttctgga tcttttaaca agagaaaaaa accaactgga
    13381 agcccagctg aagtcatcac taaatgagga aaaaaagtca aaacaacaac tggaaaaaat
    13441 cagcttactg acagacagtt tactaaaagt ctttgtaaag gacacagtca atcaactaca
    13501 acaaatcaaa aaaaccaggg atgagaaaat ccagcttagc aatcaggagc ttcttggtga
    13561 tgaccaaaag aaagtaacac cccaagacct atcccaaaat gttgaggaac agtcgccaag
    13621 tatttcaggt tgcttcttaa gttctgaatt ggaagatgaa aaagaagaga tttcctctcc
    13681 agatatgtgt cccagaccgg agagcccagt atttggtgcc agtgggcagg aagaacttgc
    13741 taagagactt gctgaacttg aactcagccg ggagttcctg agcgcgttag gagatgatca
    13801 agactggttt gatgaagact ttggtttgag ctcttctcac aagatccaaa aaaataaggc
    13861 agaagaaacc attgtacctc taatggcaga acctaaaaga gtaacccaac aaccatgtga
    13921 aacattattg gcagtccccc atactgcaga agaagtagag attcttgtac ataatgcagc
    13981 agaagaactt tggaaatgga aagaattagg ccacgatctt catagcatca gtattcctac
    14041 aaaactgctt ggctgtgcca gtaaaggtct agatatagaa agcactagta aaagggtcta
    14101 caaacaggcg gtttttgatt taacaaaaga gatttttgag gaaatatttg ctgaggatcc
    14161 caacttaaat caacctgtct ggatgaagcc atgtagaatc aactctagtt atttccgacg
    14221 agtgaaaaat ccaaataacc ttgatgaaat caagagcttc atagcaagtg aagtactcaa
    14281 gttgttcagt cttaaaaagg agccaaacca caaaacagat tggcagaaaa tgatgaaatt
    14341 tggaagaaag aaaagagacc gagtggatca tatcctggtt caggagctcc atgaggagga
    14401 ggcacagtgg gtgaactatg atgaggatga gttgtgtgtg aaaatgcagc tagccgacgg
    14461 gatctttgag acccgatcaa agatactatt gatgttctga atcagatcag tgaaaagcag
    14521 gggagaatgc tactagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc
    14581 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
    14641 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
    14701 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
    14761 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
    14821 ggcggaaaga accagctggg gctctagggg gtatcccc
//
