LOCUS       sequence-265904-        9802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9802)
  AUTHORS   Rosonina E, Blencowe BJ
  TITLE     Analysis of the requirement for RNA polymerase II CTD heptapeptide 
            repeats in pre-mRNA splicing and 3'-end cleavage.
  JOURNAL   RNA. 2004 Apr;10(4):581-9.
  PUBMED    15037767
REFERENCE   2  (bases 1 to 9802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA."; 
            date: "2004-04"; volume: "10(4)"; pages: "581-9"
COMMENT     Sequence Label: FLAG-Pol-II-Delta
FEATURES             Location/Qualifiers
     source          1..9802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             907..930
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      1476..1485
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1879..1888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5810..5827)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5816..6040
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6086..6514
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6173..6192)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6383..6404
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6523..6543)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6528..6857
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6708..6843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6770..6789
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6905..6952
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6971..7345
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7475..7596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7512..7531)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7566..7585
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7645..7661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7645..7661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7658..7680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7669..7685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7693..7699,7700..7717,7718..7723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7738..7759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7876..7893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8047..8635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8127..8146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8806..9597,9598..9666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9429..9448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9667..9771)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
      901 accatggact acaaggacga cgatgacaag gctagcatgc acgggggtgg ccccccctcg
      961 ggggacagcg catgcccgct gcgcaccatc aagagagtcc agttcggagt cctgagtccg
     1021 gatgaactga agcgaatgtc tgtgacggag ggtggcatca aatacccaga gacgactgag
     1081 ggaggccgcc ccaagcttgg ggggctgatg gacccgaggc agggggtgat tgagcggact
     1141 ggccgctgcc aaacatgtgc aggaaacatg acagagtgtc ctggccactt tggccacatt
     1201 gaactggcca agcctgtgtt tcacgtgggc ttcctggtga agacaatgaa agttttgcgc
     1261 tgtgtctgct tcttctgctc caaactgctt gtggactcta acaacccaaa gatcaaggat
     1321 atcctggcta agtccaaggg acagcccaag aagcggctca cacatgtcta cgacctttgc
     1381 aagggcaaaa acatatgcga gggtggggag gagatggaca acaagttcgg tgtggaacaa
     1441 cctgagggtg acgaggatct gaccaaagaa aagggccatg gtggctgtgg gcggtaccag
     1501 cccaggatcc ggcgttctgg cctagagctg tatgcggaat ggaagcacgt taatgaggac
     1561 tctcaggaga agaagatcct gctgagtcca gagcgagtgc atgagatctt caaacgcatc
     1621 tcagatgagg agtgttttgt gctgggcatg gagccccgct atgcacggcc agagtggatg
     1681 attgtcacag tgctgcctgt gcccccgctc tccgtgcggc ctgctgttgt gatgcagggc
     1741 tctgcccgta accaggatga cctgactcac aaactggctg acatcgtgaa gatcaacaat
     1801 cagctgcggc gcaatgagca gaacggcgca gcggcccatg tcattgcaga ggatgtgaag
     1861 ctcctccagt tccatgtggc caccatggtg gacaatgagc tgcctggctt gccccgtgcc
     1921 atgcagaagt ctgggcgtcc cctcaagtcc ctgaagcagc ggttgaaggg caaggaaggc
     1981 cgggtgcgag ggaacctgat gggcaaaaga gtggacttct cggcccgtac tgtcatcacc
     2041 cccgacccca acctctccat tgaccaggtt ggcgtgcccc gctccattgc tgccaacatg
     2101 acctttgcgg agattgtcac ccccttcaac attgacagac ttcaagaact agtgcgcagg
     2161 gggaacagcc agtacccagg cgccaagtac atcatccgag acaatggtga tcgcattgac
     2221 ttgcgtttcc accccaagcc cagtgacctt cacctgcaga ccggctataa ggtggaacgg
     2281 cacatgtgtg atggggacat tgttatcttc aaccggcagc caactctgca caaaatgtcc
     2341 atgatggggc atcgggtccg cattctccca tggtctacct ttcgcttgaa tcttagcgtg
     2401 acaactccgt acaatgcaga ctttgacggg gatgagatga acttgcacct gccacagtct
     2461 ctggagacgc gagcagagat ccaggagctg gccatggttc ctcgcatgat tgtcaccccc
     2521 cagagcaatc ggcctgtcat gggtattgtg caggacacac tcacagcagt gcgcaaattc
     2581 accaagagag acgtcttcct ggagcggggt gaagtgatga acctcctgat gttcctgtcg
     2641 acgtgggatg ggaaggtccc acagccggcc atcctaaagc cccggcccct gtggacaggc
     2701 aagcaaatct tctccctcat catacctggt cacatcaatt gtatccgtac ccacagcacc
     2761 catcccgatg atgaagacag tggcccttac aagcacatct ctcctgggga caccaaggtg
     2821 gtggtggaga atggggagct gatcatgggc atcctgtgta agaagtctct gggcacgtca
     2881 gctggctccc tggtccacat ctcctaccta gagatgggtc atgacatcac tcgcctcttc
     2941 tactccaaca ttcagactgt cattaacaac tggctcctca tcgagggtca tactattggc
     3001 attggggact ccattgctga ttctaagact taccaggaca ttcagaacac tattaagaag
     3061 gccaagcagg acgtaataga ggtcatcgag aaggcacaca acaatgagct ggagcccacc
     3121 ccagggaaca ctctgcggca gacgtttgag aatcaggtga accgcattct taacgatgcc
     3181 cgagacaaga ctggctcctc tgctcagaaa tccctgtctg aatacaacaa cttcaagtct
     3241 atggtcgtgt ccggagctaa aggttccaag attaacatct cccaggtcat tgctgtcgtt
     3301 ggacagcagg acgtcgaggg caagcggatt ccatttggct tcaagcaccg gactctgcct
     3361 cacttcatca aggatgacta cgggcctgag agccgtggct ttgtggagaa ctcctaccta
     3421 gccggcctca cacccactga gttctttttc cacgccatgg ggggtcgtga ggggctcatt
     3481 gacacggctg tcaagactgc tgagactgga tacatccagc ggcggctgat caagtccatg
     3541 gagtcagtga tggtgaagta cgacgcgact gtgcggaact ccatcaacca ggtggtgcag
     3601 ctgcgctacg gcgaagacgg cctggcaggc gagagcgttg agttccagaa cctggctacg
     3661 cttaagcctt ccaacaaggc ttttgagaag aagttccgct ttgattatac caatgagagg
     3721 gccctgcggc gcactctgca ggaggacctg gtgaaggacg tgctgagcaa cgcacacatc
     3781 cagaacgagt tggagcggga atttgagcgg atgcgggagg atcgggaggt gctcagggtc
     3841 atcttcccaa ctggagacag caaggtcgtc ctcccctgta acctgctgcg gatgatctgg
     3901 aatgctcaga aaatcttcca catcaaccca cgccttccct ccgacctgca ccccatcaaa
     3961 gtggtggagg gagtcaagga attgagcaag aagctggtga ttgtgaatgg ggatgaccca
     4021 ctaagtcgac aggcccagga aaatgccacg ctgctcttca acatccacct gcggtccacg
     4081 ttgtgttccc gccgcatggc agaggagttt cggctcagtg gggaggcctt cgactggctg
     4141 cttggggaga ttgagtccaa gttcaaccaa gccattgcgc atcccgggga aatggtgggg
     4201 gctctggctg cgcagtccct tggagaacct gccacccaga tgaccttgaa taccttccac
     4261 tatgctggtg tgtctgccaa gaatgtgacg ctgggtgtgc cccgacttaa ggagctcatc
     4321 aacatttcca agaagccaaa gactccttcg cttactgtct tcctgttggg ccagtccgct
     4381 cgagatgctg agagagccaa ggatattctg tgccgtctgg agcatacaac gttgaggaag
     4441 gtgactgcca acacagccat ctactatgac cccaaccccc agagcacggt ggtggcagag
     4501 gatcaggaat gggtgaatgt ctactatgaa atgcctgact ttgatgtggc ccgaatctcc
     4561 ccctggctgt tgcgggtgga gctggatcgg aagcacatga ctgaccggaa gctcaccatg
     4621 gagcagattg ctgaaaagat caatgctggt tttggtgacg acttgaactg catctttaat
     4681 gatgacaatg cagagaagct ggtgctccgt attcgcatca tgaacagcga tgagaacaag
     4741 atgcaagagg aggaagaggt ggtggacaag atggatgatg atgtcttcct gcgctgcatc
     4801 gagtccaaca tgctgacaga tatgaccctg cagggcatcg agcagatcag caaggtgtac
     4861 atgcacttgc cacagacaga caacaagaag aagatcatca tcacggagga tggggaattc
     4921 aaggccctgc aggagtggat cctggagacg gacggcgtga gcttgatgcg ggtgctgagt
     4981 gagaaggacg tggaccccgt acgcaccacg tccaatgaca ttgtggagat cttcacggtg
     5041 ctgggcattg aagccgtgcg gaaggccctg gagcgggagc tgtaccacgt catctccttt
     5101 gatggctcct atgtcaatta ccgacacttg gctctcttgt gtgataccat gacctgtcgt
     5161 ggccacttga tggccatcac ccgacacgga gtcaaccgcc aggacacagg accactcatg
     5221 aagtgttcct ttgaggaaac ggtggacgtg cttatggaag cagccgcaca cggtgagagt
     5281 gaccccatga agggggtctc tgagaatatc atgctgggcc agctggctcc ggccggcact
     5341 ggctgctttg acctcctgct tgatgcagag aagtgcaagt atggcatgga gatccccacc
     5401 aatatccccg gcctgggggc tgctggaccc accggcatgt tctttggttc agcacccagt
     5461 cccatgggtg gaatctctcc tgccatgaca ccttggaacc agggtgcaac ccctgcctat
     5521 ggcgcctggt cccccagtgt tgggagtgga atgaccccag gggcagccgg cttctctccc
     5581 agtgctgcgt cagatgccag cggcttcagc ccaggttact cccctgcctg gtctcccaca
     5641 ccgggctccc cggggtcccc aggtccctca agcccctaca tcccttcacc aggtggcgcc
     5701 atgtctccca gctctagata aatcgattaa gcggccgcct aagaattcca ccacactgga
     5761 ctagtggatc cgagctcggt accaagctta agtttaaacc gctgatcagc ctcgactgtg
     5821 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     5881 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     5941 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     6001 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     6061 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     6121 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     6181 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     6241 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     6301 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     6361 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     6421 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     6481 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     6541 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6601 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6661 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6721 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6781 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6841 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc
     6901 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag
     6961 gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc
     7021 cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga
     7081 cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt
     7141 ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga
     7201 gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat
     7261 cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca
     7321 cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt
     7381 ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg
     7441 cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg
     7501 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     7561 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc
     7621 tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     7681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     7741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     7801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     8581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     8641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     8701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     8761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     8821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9421 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     9601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     9661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     9721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     9781 ccgaaaagtg ccacctgacg tc
//
