LOCUS       sequence-265926-        9920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible expression of human ETV6 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9920)
  AUTHORS   Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark 
            NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, 
            Lemischka IR, Moore K
  TITLE     Induction of a hemogenic program in mouse fibroblasts.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 
            10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.
  PUBMED    23770078
REFERENCE   2  (bases 1 to 9920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.05.024"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "205-18"
COMMENT     Sequence Label: pFUW-TetO-ETV6
FEATURES             Location/Qualifiers
     source          1..9920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1443..1676
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1861..1905
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2203..2320
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2377..2395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2419..2437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2503..2521
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2545..2563
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2596..2634
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2631..2655
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     misc_feature    4375..4963
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4428..4448)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4846..4857)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4966..4982)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4967..4983)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5488..5668
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5694..5711)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5700..5924
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5970..6398
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6057..6076)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6267..6288
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6407..6427)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6412..6741
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6592..6727
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6654..6673
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6789..6836
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6855..7229
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7359..7480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7396..7415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7450..7469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7529..7545)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7529..7545)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7542..7564)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7553..7569
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7577..7583,7584..7601,7602..7607))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7622..7643
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7760..7777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7931..8519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8011..8030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8690..9481,9482..9550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9313..9332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9551..9655)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9730..9749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     1321 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     1381 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     1441 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     1501 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     1561 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     1621 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     1681 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     1741 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     1801 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     1861 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     1921 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     1981 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2041 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2101 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     2161 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     2221 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     2281 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
     2341 cagagatcca gtttggttag atctcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     2521 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagct
     2581 cggtacccgg gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
     2641 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
     2701 cgggaccgat ccagcctccg cggccccgaa ttcatgtctg agactcctgc tcagtctagc
     2761 attaagcctc gagggactgg ttcatgctgt cctcacgagg aagggcctca cccctgagtc
     2821 cacagcttca gatggctgag attaccagga aatagcctca gagaagaatg ctgagataat
     2881 aggaacctcg tgatccagga aagagtatat gcagaattaa cacagcagga acgaatttca
     2941 tacacgcccc cagagagtcc agtggcaagc caccgttcct cgactccgct tcatgttcac
     3001 acagtgcctc gagcgctcag gatggaggaa gactcgatcc acctgccaac acacctgcgt
     3061 ttgcagccga tttactggag cagagatgac gtagcccagt ggctcaagtg ggcagaaaat
     3121 gagttttctt taaggcccat tgagagcaac aagttcgaaa tgaatggcaa ggccctcctg
     3181 ctgctgacca aagaggattt ccgctaccga tctcctcatt caggcgacgt gctctatgaa
     3241 ctccttcagc atatcctgaa gcagaggaaa tctcgaatgc tcttctcacc attcttcccc
     3301 cctggggact ctatccacac caagccagag gtcctcctgc atcagaacca tgacgaagat
     3361 aactgtgtcc agaggacacc caggacgccc gcggagagcg tgcaccacaa ccctcccacc
     3421 atcgaactct tacatcgccc taggtcaccc atcaccacaa accacaggcc ttctcctgac
     3481 cccgaacagc agcggcccca gcggtccccc ctagacaaca tgagccgccg cctctcgcca
     3541 gtggagaaag cccaggggcc caggctacag caggagaaca accaccagga aacgtacccc
     3601 ctgtcagtgt ctcctgtcga gaataatcac tgcctgccct caagcccctg gcaggagagc
     3661 actcgagtga tccagctgat gcccagcccc atcatgcacc ctttgatcct gaacccccgg
     3721 cactcgcact cggtggactt caaacagtcc cggcactccg aggatgggat gaatcgggaa
     3781 gggaagccca tcaacctgtc tcatcgggag gacctggctt acttgaacca catcatggtc
     3841 tctatgtccc caccggaaga gcacgccatg cccattggga gaatagcaga ctgtagactg
     3901 ctttgggatt atgtctatca gttgctgtct gacagccggt acgaaaactt catccgatgg
     3961 gaggacaaag aatccaaaat attccggata gtggatccca acggactggc tcgactctgg
     4021 ggaaaccata agaacagaac aaacatgacc tatgagaaaa tgtccagagc cctgcgccac
     4081 tactacaaac taaacattat caggaaggag cccggacaaa ggcttttgtt caggttcatg
     4141 aaaaccccag atgagatcat gagtggccgg acagaccgtc tagaacacct cgagtctcaa
     4201 gtgctggatg aacaaacgta ccaagaggat gaacctacca tagcctcacc ggtgggctgg
     4261 ccaagaggaa acctgcccac ggggaccgca ggaggcgtga tggaagcagg cgagctaggg
     4321 gtggctgtaa aggaagagac ccgggaatag gaattcgata tcaagcttat cgataatcaa
     4381 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4441 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4501 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4561 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4621 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4681 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4741 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     4801 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4861 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     4921 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     4981 cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac caatgctgat
     5041 tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac acctcaggta
     5101 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     5161 gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga tctgtggatc
     5221 taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc agggatcaga
     5281 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa
     5341 gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca tgggatggat
     5401 gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt tcatcacatg
     5461 gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     5521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5641 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     5701 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     5761 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     5821 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     5881 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     5941 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     6001 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     6061 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     6121 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     6181 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     6241 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     6301 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     6361 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     6421 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6481 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     6541 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     6601 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     6661 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     6721 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     6781 cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa tacgacaagg
     6841 tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg
     6901 tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg
     6961 acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg gtccaggacc
     7021 aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac gagctgtacg
     7081 ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg gccatgaccg
     7141 agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg
     7201 tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat tccaccgccg
     7261 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     7321 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     7381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     7501 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     7561 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     7621 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     7681 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     7741 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     7801 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     7861 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     7921 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     7981 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8041 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8101 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8161 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8221 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     8281 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     8341 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     8401 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     8461 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     8521 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     8581 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     8641 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     8701 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     8761 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     8821 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     8881 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     8941 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9001 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9061 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9121 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9181 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9241 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     9301 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     9361 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     9421 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     9481 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     9541 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     9601 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     9661 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     9721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     9781 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     9841 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     9901 acgggccaga tatacgcgtt
//
