LOCUS       sequence-265927-       10323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Communication-activated luciferase in Neurospora crassa.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10323)
  AUTHORS   Fischer MS, Jonkers W, Glass NL
  TITLE     Integration of Self and Non-self Recognition Modulates Asexual 
            Cell-to-Cell Communication in Neurospora crassa.
  JOURNAL   Genetics. 2019 Apr;211(4):1255-1267. doi: 
            10.1534/genetics.118.301780. Epub 2019 Feb 4.
  PUBMED    30718271
REFERENCE   2  (bases 1 to 10323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.118.301780"; journalName: "Genetics"; date: 
            "2019-04"; volume: "211"; issue: "4"; pages: "1255-1267"
COMMENT     Sequence Label: Pprm1-luciferase; his-3
FEATURES             Location/Qualifiers
     source          1..10323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(98..117)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     217..239
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(277..295)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        363..467
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(468..536,537..1328)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(686..705)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1988..2007
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2241..2258
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4008..4026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7597..7613)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7598..7614)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(7643..7661)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(10322..10323,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(join(10323,1..17))
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgctc catcttcctc ctggtcgcat
     4081 acttcatgac agcacggcga tgttcaataa cagcgaagtg aaggggacgc cggcggaggt
     4141 gaggtgaaga tggtgaagac gtcggggcct ctcacgtgcc tctgacgcac ggatcatcac
     4201 tctgcctgcc gcttcaacaa gtacctatga atggacatgg ccaaatgcaa atccccatgg
     4261 accaaacaac aagcggcggc cgtcgactgt tgaatgaacg cggcaatcca tttttctgcg
     4321 tgagtcattt gaacggattc gtcattcaga gtctttattt aacatttttg aaacagcaga
     4381 atggaagcgc ccggctgctg gcctctccat ctctccccgg ctggggcttg tcgggtcagt
     4441 tgtcgtcatt gggctttcag aggctccggg gcattcactg gctgtcaggg ctccagcggg
     4501 tagtggcccc actttccact tggcatcggc agaagtggaa gcgcgtctgt tggacccttg
     4561 tcaggcacca atcactggcc ggagacagcc cgcggttgaa acgcaggaac gccttccact
     4621 tttgagtgcc accttctttt ttttttcttc ctctttttct tcctcttttc cacatcccgg
     4681 cactcattgc atttcagcat cggaactaaa caccaggatg agctgaggcc agatctggcg
     4741 accggcctcg attgcaaccc aacatctgct ctgccacaga ctccagatca gagaggctga
     4801 tggtggtgaa ggacatcacg gtgacccatt gtcaaaggtg gaatacagga ccggcacaca
     4861 tcgttcacct tgccgtgagt atgatgatga gccctcccga gtcccatcag gtggccttgt
     4921 ctcatgaaca cacagcatgg cccggctgca agcgcgccag acatcaagac gacaggctca
     4981 cattgatcca tcaacccagc cagtttttga tgggctgcct ctccaccaag gaaatccggg
     5041 gtgttcaagg atcaatccat gacaaagaac gactggctgc cagagtccgg gcgcttagac
     5101 aaccaccgcg gctacgggac gactacccct tgatcagcct cctcccactc cactcactgt
     5161 tcacgcacac agattcaaac aaaggcaatg tgctttcaag aaacaatggg ccttcttcct
     5221 attctgggac ccggtgcgcc tattgtcatt tgcacatcac ctgctatccc atcttttggt
     5281 ccttcatccc aactccggtc ccaggtctca acttattcac tgatccacca gcctccggga
     5341 gtccgggcca gtcaagtcac tagcctgcct gggtccgtct tattctcccg tccgtcgtct
     5401 tattgacgca ctttctttct attgttcact cacctcctca cctgttcgtt tcccattctt
     5461 cacctattca tatctccagt atgcagtcat agtgggcaag cctttctgat ttggataccc
     5521 tcttgtttcc tttgactcac ttctcttgtt cctttctctt gctcatttgc atgtcctttt
     5581 ttcctttttc ccggtcttcg gctcagtaat tctttctcga gagtgttcat ccagctggtc
     5641 tagacgagtg aacagcagtt ttctgtcttt ttttttctcc tagcagacat acaccacgcg
     5701 ctctcacggc attcgcttga cttttcactt ttcgactttt gcacgattag tagacaacta
     5761 cacgatatac tacaccatct tgctggttca agaataagaa cgatatatca aaattggatc
     5821 cccgggttaa ttaacatgga ggacgccaag aacatcaaga agggccccgc ccccttctac
     5881 cccctcgagg acggcaccgc cggcgagcag ctccacaagg ccatgaagcg ctacgccctc
     5941 gtccccggca ccatcgcctt cgtaggtttc ctccagctct cgcctccagc acccgaggca
     6001 catctcgggc atcttcacaa caacagacac tgacatctca ttctcacaga ccgacgccca
     6061 catcgaggtc aacatcacct acgccgagta cttcgagatg tccgtccgcc tcgccgaggc
     6121 catgaagcgc tacggcctca acaccaacca ccgcatcgtc gtctgctccg agaactccct
     6181 ccagttcttc atgcccgtcc tcggcgccct cttcatcggc gtcgccgtcg cccccgccaa
     6241 cgacatctac aacgagcgcg agctcctcaa ctccatgaac atctcccagc ccaccgtcgt
     6301 cttcgtctcc aagaagggcc tccagaagat cctcaacgtc cagaagaagc tccccatcat
     6361 ccagaagatc atcatcatgg actccaagac cgactaccag ggcttccagt ccatgtacac
     6421 cttcgtcacc tcccacctcc cccccggctt caacgagtac gacttcgtcc ccgagtcctt
     6481 cgaccgcgac aagaccatcg ccctcatcat gaactcctcc ggctccaccg gcctccccaa
     6541 gggcgtcgcc ctcccccacc gcaccgcctg cgtccgcttc tcccacgccc gcgaccccat
     6601 cttcggcaac cagatcatcc ccgacaccgc catcctctcc gtcgtcccct tccaccacgg
     6661 cttcggcatg ttcaccaccc tcggctacct catctgcggc ttccgcgtcg tcctcatgta
     6721 ccgcttcgag gaggagctct tcctccgctc cctccaggac tacaagatcc agtccgccct
     6781 cctcgtcccc accctcttct ccttcttcgc caagtccacc ctcatcgaca agtacgacct
     6841 ctccaacctc cacgagatcg cctccggcgg cgcccccctc tccaaggagg tcggcgaggc
     6901 cgtcgccaag cgcttccacc tccccggcat ccgccagggc tacggcctca ccgagaccac
     6961 ctccgccatc ctcatcaccc ccgagggcga cgacaagccc ggcgccgtcg gcaaggtcgt
     7021 ccccttcttc gaggccaagg tcgtcgacct cgacaccggc aagaccctcg gcgtcaacca
     7081 gcgcggcgag ctctgcgtcc gcggccccat gatcatgtcc ggctacgtca acaaccccga
     7141 ggccaccaac gccctcatcg acaaggacgg ctggctccac tccggcgaca tcgcctactg
     7201 ggacgaggac gagcacttct tcatcgtcga ccgcctcaag tccctcatca agtacaaggg
     7261 ctaccaggtc gcccccgccg agctcgagtc catcctcctc cagcacccca acatcttcga
     7321 cgccggcgtc gccggcctcc ccgacgacga cgccggcgag ctccccgccg ccgtcgtcgt
     7381 cctcgagcac ggcaagacca tgaccgagaa ggagatcgtc gactacgtcg cctcccaggt
     7441 caccaccgcc aagaagctcc gcggcggcgt cgtcttcgtc gacgaggtcc ccaagggcct
     7501 caccggcaag ctcgacgccc gcaagatccg cgagatcctc atcaaggcca agaagggcgg
     7561 caagtccaag ctctgagaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg
     7621 gcccggtacc cagcttttgt tccctttagt gagggttaat tagggcgtgg acggctaatg
     7681 gggtctgaat gctaaaagac accatttccc acactccctc tgattttttt tatggttttt
     7741 ttttatcttc atttttcgcc gtcttttatt cggttgtttg ggaggttcac atgcaacgtg
     7801 caagactgca agttttttga gatgggcgct tcatgcaatg cagcatcact gcagggacat
     7861 tgtgacatcc acgtttcgat cgtctgtgct tcccccgttc atgtacgcat gatctgaaaa
     7921 aaaaaaaaaa aaaaaaggtt gatgtaataa tgaaaataaa cacccaggga cgagcacctc
     7981 atctgctgga atagggagaa aaactcaagg gccgtttggg tctccacact taccaacaga
     8041 ttcatgtact gatcttcagc ctgtacagaa cagtacaatg tccaaaactc gttatcatta
     8101 ctctatgtgc ccaaaatgac ccagatatac atcaacccac tacatccttt tgttcttgtt
     8161 tttttttttt ttttttgcta cttcgaaaag aatttccgtt tagttgctct tgctcatgtg
     8221 ctcaagacgg atgctgaccg cccttctgtg agcctcgagc tcctcaacct tagccaactg
     8281 catgacagcc tggccgacgt ttttgagacc ctcggcagtc aagttggagc tggtaatgtg
     8341 cttgacgaac gaggcgaaat tgacgccaga gtactgcttg ccaaagccat aggtagctat
     8401 gtgtcttgac gttagcagaa gaatctgtga agcggggcta tttatgatat gtaacttacg
     8461 cagcgagtgg ttaacaccag cagagtaatc gccaacggac tcaggagtcc aggcgccaat
     8521 gaagacacta ccggcgttca tgacaagatc gacagccttc tcggcctcct tgatctggag
     8581 gatcaagtgc tcaggagcgt acttgttgct gagctccatg gcctcctcga cggtcttgac
     8641 ctgcacggtg atcgagtggg cgatggagcc acggacaatc tggacgcgag gaagctccgt
     8701 agcctgacgg tgaacctcgt cctcaatagc ctgaagatgc tcctcgtcga ggtcaatagc
     8761 gatcaggatg acctgactgt caacgccgtg ctcagcctgg gacaggagat ccgaggcaac
     8821 gaacgcgggg ttggcgtcct tgtcagcgat gaccagcacc tcggacgggc cagcgggcat
     8881 gtcaatacca acggcagcgt tggtgtcgtt gctgacgaac atcttggcag cagtgacgaa
     8941 ctggttaccg gggccgagaa tcttgtcgac cttggtgatg ctctcggtgc cgtaggccat
     9001 ggcagctacg gcctgggcac cgccggcaag cacgatggac tcggccccaa ccttgtgagc
     9061 gacgtggaca atctcgggag tgatggttcc gtcggcgcgg ggaggagagg cgaacacaat
     9121 cttgttgcag ccggcgacca tggcgggaac acccagcata agggcagtgc tggggagaac
     9181 ggcggtaccg ccggggatgt agcagccgac ggcctcgatg ggacgagaga aacggctgca
     9241 gacaacaccg ggcatggtct cgacctggag gggcttctcc tccttctggg cggcgtggaa
     9301 cttgcggatg ttctcgaagg acacgtcgat ggcagcaatg gtctcctcag ggagctgcat
     9361 aagctccttg gggaagggcg ccttcaggac ggggctagta agagaggtag ccttctcgaa
     9421 cttgtgagtg tacgacagaa cagccttgtc gccgttcttg cggacgtcct cgatgatggg
     9481 gacaatgatc ttgtagatgg catcggacga cttttgcgca ggacgcttga gagcagcatc
     9541 gagctcctcg gtagagacct tggaggcgtc gaaacgtctc atggtgatct tctcaggggt
     9601 ggtctcctgg gcggcctcct tggtgacagg ggccgaagtg gcagcgagag cggacgccgc
     9661 aggcttgatg ccctccttct cagcccactt acccttagca tctccagtcc tgcgcttgac
     9721 cttccagctc ttggcgtcaa ggctcctttc gatatcggca agagtaacgc cggcagcaac
     9781 ggccctggta agagcaaagt agaagagatc ggcagcctca aaggcgattt cctggggggt
     9841 ctgagcggtg cagagctcct cagcctcctc catgatcttg gcccggacta gcttctcatc
     9901 ggagaagaga cgggcagtgt aggagccctc gggggcagac tgtttcctcg aaatcaaagt
     9961 ctgctcgagc ttgggaaggc ctttgagctg accaaagcag ccggactgat cgaggtggca
    10021 gaaacgaccc ttctgcttca cgacaaactt gagagcatcg ttatcgcagt caagcgagat
    10081 gcggacgagc tcctgagtgt ctccggaagt agcacccttg taccagagac cgcgcttccg
    10141 gctctgatag acaccagtct gtgtcctgag ggcctcgttc acactctcgg cactgctgta
    10201 gaccagaccc agagcagtgt cgtgctcatc aacgacaacg gtggggagaa gaccatcggg
    10261 acggtcagac ttccagacgc tcgagaggat ggtggagatg gcaagcttca gctgctcgag
    10321 ttc
//
