LOCUS       sequence-265934-       13322 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Replication helper.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Zhang Q, Zhang Y, Lu MH, Chai YP, Jiang YY, Zhou Y, Wang XC, Chen QJ
  TITLE     A Novel Ternary Vector System United with Morphogenic Genes Enhances
            CRISPR/Cas Delivery in Maize.
  JOURNAL   Plant Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 
            10.1104/pp.19.00767.
  PUBMED    31558579
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol. 2019 Sep 26. pii: pp.19.00767. doi: 10.1104/pp.19.00767."
COMMENT     Sequence Label: pVS1-SAH2
FEATURES             Location/Qualifiers
     source          1..13322
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             278..1069
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1325..1913
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1814..1833
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2067..2084
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2099..2239
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2165..2184)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2325..2347
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      2583..2777
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(2843..3916)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(4345..4974)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8025..9173
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
ORIGIN
        1 aattcgaacc cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat
       61 gcgctcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc
      121 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa
      181 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac
      241 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga
      301 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt
      361 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat
      421 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa
      481 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt
      541 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca
      601 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga
      661 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc
      721 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga
      781 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct
      841 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc
      901 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag
      961 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga
     1021 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag
     1081 aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta
     1141 agcacataat tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg
     1201 tgcataataa gcccaaggcg cgcctacaca aattgggaga tatatcatgc atgaccaaaa
     1261 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     1321 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     1381 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     1441 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     1501 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     1561 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     1621 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     1681 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     1741 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     1801 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     1861 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     1921 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     1981 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     2041 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     2101 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     2161 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
     2221 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     2281 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     2341 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc
     2401 ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc
     2461 cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg gggcgtaggg
     2521 agcacagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca
     2581 gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag ttttaggcgg
     2641 aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg ttcccaatgt
     2701 acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt gggttcccaa
     2761 tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct agggcaattt
     2821 gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg atcaggttgc
     2881 ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa tcgtactccg
     2941 gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg aactctccgg
     3001 cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg
     3061 cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg
     3121 ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac atccaatcag
     3181 ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc ttgtagcggc
     3241 taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc ttcttcgtac
     3301 gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct
     3361 gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca
     3421 cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg gttagatggg
     3481 aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc ggccctgcgg
     3541 aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct cgtcggtcac
     3601 gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt
     3661 catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg
     3721 acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca gcggccgctt
     3781 gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg
     3841 ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc gggccggatg
     3901 gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
     3961 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
     4021 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
     4081 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
     4141 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
     4201 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
     4261 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
     4321 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
     4381 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
     4441 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
     4501 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
     4561 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
     4621 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
     4681 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
     4741 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
     4801 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
     4861 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
     4921 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
     4981 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
     5041 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
     5101 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
     5161 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
     5221 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
     5281 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
     5341 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
     5401 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
     5461 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
     5521 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
     5581 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
     5641 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
     5701 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
     5761 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
     5821 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
     5881 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
     5941 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
     6001 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
     6061 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
     6121 cgactgcact atagcttaat tcatcgcgtc ccccttggtc aaattgggta tacccatttg
     6181 ggcctagtct agccggcatg gcgcattaca gcaatacgca atttaaatgc gcctagcgca
     6241 ttttcccgac cttaatgcgc ctcgcgctgt agcctcacgc ccacatatgt gctaatgtgg
     6301 ttacgtgtat tttatggagg ttatccaatg agccgcctga caatcgacat gacggaccag
     6361 cagcaccaga gcctgaaagc cctggccgcc ttgcagggca agaccattaa gcaatacgcc
     6421 ctcgaacgtc tgttccccgg tgacgctgat gccgatcagg catggcagga actgaaaacc
     6481 atgctgggga accgcatcaa cgatgggctt gccggcaagg tgtccaccaa gagcgtcggc
     6541 gaaattcttg atgaagaact cagcggggat cgcgcttgac ggcctacatc ctcacggctg
     6601 aggccgaagc cgatctacgc ggcatcatcc gctacacgcg ccgggagtgg ggcgcggcgc
     6661 aggtgcgccg ctatatcgct aagctggaac agggcatagc caggcttgcc gccggcgaag
     6721 gcccgtttaa ggacatgagc gaactctttc ccgcgctgcg gatggcccgc tgcgaacacc
     6781 actacgtttt ttgcctgccg cgtgcgggcg aacccgcgtt ggtcgtggcg atcctgcatg
     6841 agcgcatgga cctcatgacg cgacttgccg acaggctcaa gggctgattt cagccgctaa
     6901 aaatcgcgcc actcacaacg tcctgatggc gtacttaatt aacgacttta ggtccaagct
     6961 taatcgaacc gcacgacgta ggggttctga tagaaaacaa ctgtcaaagc gcacccgccc
     7021 gatgccattc gcggcacggc ttccgttgag gatgtcgata tgatgcgcga gccgacggcc
     7081 cgcagagaag gggccgtttt agcggctaaa gaaggaagtg caagccctaa cccttggcgt
     7141 cagagccttc cacgcagctt ttttcgggtg tcgtcgcccc atttctttac gataaacgcc
     7201 ttatgtgacg gcaaaaccac actgatgcgt tcgtatccgg gcggcacgct gctcttgaaa
     7261 ggatgacccg caatctccgc gagtgcctcg cggtcaaggt cggtggactc caggagaaga
     7321 ggtaggggag tttccagggc gtcggcaatg gcctccatca ccttcaacga ggggttggcc
     7381 ttaccgttgg ttaagtctga taaaaacgaa attgaaaccc ctgccctctc cgacagctca
     7441 tgtttcgtca tgccccgctc atcgagcaga cgaaggatgt tggtgaaaaa tatctggttg
     7501 tacacagcgg aagccgcccc tcgcaccttt ggtcgcggcc cgcaaaattt tagccgctaa
     7561 agttcttgac agcggaacca atgtttagct aaactagagt ctcctttctc aaggagactt
     7621 tcgatatgag ccataatcag ttccagttta tcggtaatct tacccgtgac accgaggtac
     7681 gtcatggcaa ttctaacaag ccgcaagcaa ttttcgatat agcggttaat gaagagtggc
     7741 gcaacgatgc cggcgacaag caggagcgca ccgacttctt ccgcatcaag tgttttggct
     7801 ctcaggccga ggcccacggc aagtatttgg gcaaggggtc gctggtattc gtgcagggca
     7861 agattcggaa taccaagtac gggaaggacg gccagacggt ctacgggacc gacttcattg
     7921 ccgataaggt ggattatctg gacaccaagg caccaggcgg gtcaaatcag gaataagggc
     7981 acattgcccc ggcgtgagtc ggggcaatcc cgcaaggagg gtgaatgaat cggacgtttg
     8041 accggaaggc atacaggcaa gaactgatcg acgcggggtt ttccgccgag gatgccgaaa
     8101 ccatcgcaag ccgcaccgtc atgcgtgcgc cccgcgaaac cttccagtcc gtcggctcga
     8161 tggtccagca agctacggcc aagatcgagc gcgacagcgt gcaactggct ccccctgccc
     8221 tgcccgcgcc atcggccgcc gtggagcgtt cgcgtcgtct cgaacaggag gcggcaggtt
     8281 tggcgaagtc gatgaccatc gacacgcgag gaactatgac gaccaagaag cgaaaaaccg
     8341 ccggcgagga cctggcaaaa caggtcagcg aggccaagca ggccgcgttg ctgaaacaca
     8401 cgaagcagca gatcaaggaa atgcagcttt ccttgttcga tattgcgccg tggccggaca
     8461 cgatgcgagc gatgccaaac gacacggccc gctctgccct gttcaccacg cgcaacaaga
     8521 aaatcccgcg cgaggcgctg caaaacaagg tcattttcca cgtcaacaag gacgtgaaga
     8581 tcacctacac cggcgtcgag ctgcgggccg acgatgacga actggtgtgg cagcaggtgt
     8641 tggagtacgc gaagcgcacc cctatcggcg agccgatcac cttcacgttc tacgagcttt
     8701 gccaggacct gggctggtcg atcaatggcc ggtattacac gaaggccgag gaatgcctgt
     8761 cgcgcctaca ggcgacggcg atgggcttca cgtccgaccg cgttgggcac ctggaatcgg
     8821 tgtcgctgct gcaccgcttc cgcgtcctgg accgtggcaa gaaaacgtcc cgttgccagg
     8881 tcctgatcga cgaggaaatc gtcgtgctgt ttgctggcga ccactacacg aaattcatat
     8941 gggagaagta ccgcaagctg tcgccgacgg cccgacggat gttcgactat ttcagctcgc
     9001 accgggagcc gtacccgctc aagctggaaa ccttccgcct catgtgcgga tcggattcca
     9061 cccgcgtgaa gaagtggcgc gagcaggtcg gcgaagcctg cgaagagttg cgaggcagcg
     9121 gcctggtgga acacgcctgg gtcaatgatg acctggtgca ttgcaaacgc tagggccttg
     9181 tggggtcagt tccggctggg ggttcagcag ccagcgcttt actggcattt caggaacaag
     9241 cgggcactgc tcgacgcact tgcttcgctc agtatcgctc gggacgcacg gcgcgctcta
     9301 cgaactgccg ataaacagag gattaaaatt gacaattgtg attaaggctc agattcgacg
     9361 gcttggagcg gccgacgtgc aggatttccg cgagatccga ttgtcggccc tgaagaaagc
     9421 tccagagatg ttcgggtccg tttacgagca cgaggagaaa aagcccatgg aggcgttcgc
     9481 tgaacggttg cgagatgccg tggcattcgg cgcctacatc gacggcgaga tcattgggct
     9541 gtcggtcttc aaacaggagg acggccccaa ggacgctcac aaggcgcatc tgtccggcgt
     9601 tttcgtggag cccgaacagc gaggccgagg ggtcgccggt atgctgctgc gggcgttgcc
     9661 ggcgggttta ttgctcgtga tgatcgtccg acagattcca acgggaatct ggtggatgcg
     9721 catcttcatc ctcggcgcac ttaatatttc gctattctgg agcttgttgt ttatttcggt
     9781 ctaccgcctg ccgggcgggg tcgcggcgac ggtaggcgct gtgcagccgc tgatggtcgt
     9841 gttcatctct gccgctctgc taggtagccc gatacgattg atggcggtcc tgggggctat
     9901 ttgcggaact gcgggcgtgg cgctgttggt gttgacacca aacgcagcgc tagatcctgt
     9961 cggcgtcgca gcgggcctgg cgggggcggt ttccatggcg ttcggaaccg tgctgacccg
    10021 caagtggcaa cctcccgtgc ctctgctcac ctttaccgcc tggcaactgg cggccggagg
    10081 acttctgctc gttccagtag ctttagtgtt tgatccgcca atcccgatgc ctacaggaac
    10141 caatgttctc ggcctggcgt ggctcggcct gatcggagcg ggtttaacct atctagactg
    10201 catgatggtg atagccgtca gcatgatttt cgtgtcgtcg aacttggtga agggcattct
    10261 cggcggcgat cacgacgccg gccctgcgga gccttcgccg cgtgcgcgat tcatggcggc
    10321 cgtggaggcc aaggatttcg cgcgagtgca agagctgatc gaggcgcgtg gagccaagtc
    10381 ggcggctgat tatgtccttg cgcagctcgc cgtggccgaa ggtctggacc gcaagcctgg
    10441 tgcgcgcgtc gtggtcggga aagcggcggg cagcatggca atgccgcctg cggcgctggg
    10501 ttttacgcca aggggagaag cggcatacgc catcgagcgg tcagcctatg gtgagccgag
    10561 gtccagcatt gcgaagcagt accagcagga atggaaccgg aaggcggcga cctggtgggc
    10621 gatggccggt gtggccggca tcatcggcgc gatcctggcg gcggcggcaa ccggctttgt
    10681 tgggctggca gtgtcgatcc gcaaccgagt gaagcgcgtg cgcgacctgt tggtgatgga
    10741 gccgggtgca gagccataag cggcaagaga cgaaagcccg gtttccgggc ttttgttttg
    10801 ttacgccaag gacgagtttt agcggctaaa ggtgttgacg tgcgagaaat gtttagctaa
    10861 acttctctca tgtgctggcg gctgtcaccg ctatgttcaa ccaaggcgcg gagcaaatta
    10921 tgggtgttat ccatgaagaa acggcttacc gaaagccagt tccaggaggc gatccagggg
    10981 ctggaagtgg ggcagcagac catcgagata gcgcggggcg tcttagtcga tgggaagcca
    11041 caggcgacgt tcgcaacgtc gctgggactg accaggggcg cagtgtcgca agcggtgcat
    11101 cgcgtgtggg ccgcgttcga ggacaagaac ttgcccgagg ggtacgcgcg ggtaacggcg
    11161 gttctgccgg aacatcaggc gtacatcgtc cggaagtggg aagcggacgc caagaaaaaa
    11221 caggaaacca aacgatgaaa actttggtca cggccaacca gaaaggcggc gtcggcaaga
    11281 cttcgaccct tgtgcatctt gccttcgact ttttcgagcg cggcttgcgg gttgccgtga
    11341 tcgacctgga cccccagggc aatgcgtcct acacgctcaa ggactttgct accggcctgc
    11401 atgcaagcaa gctgttcggc gctgtccctg ccggcggctg gaccgaaacc gcacccgcag
    11461 ccggcgacgg ccaggccgcg cgcctcgccc tcatcgagtc caacccggta ctggcgaatg
    11521 ccgaacggct gtcgctggac gacgcccgcg agctgttcgg ggcgaacatc aaggccctgg
    11581 cgaaccaagg cttcgacgtg tgcctgatcg acacggcccc gacccttggc gtcggcctgg
    11641 cggccgccct cttcgcggcc gactatgtgc tgtcccccat cgagcttgag gcgtacagca
    11701 tccagggcat caagaagatg gtcacgacca ttgcgaacgt gcgccagaag aacgccaagc
    11761 tgcaattcct tggcatggtg cccagcaagg tcgatgcgcg gaatccgcgc cacgcgcgcc
    11821 accaagccga gctgctggcc gcgtacccca agatgatgat tccggccacc gttggcctgc
    11881 gcagcagcat cgccgatgcc ctcgcatccg gtgtgccggt ctggaagatc aagaaaacgg
    11941 ccgcgcgcaa ggcatcgaaa gaggttcgcg ccctggctga ttacgtgttc acgaagatgg
    12001 agatttccca atgactgcgg ctcaagccaa gaccaccaag aaaaacaccg ctgcggccgc
    12061 tcaggaagcc gcaggcgcgg cgcagccgtc cggcctgggg ttggatagca tcggcgacct
    12121 gtcgagcctc ctggacgctc ctgcggcgtc tcagggcggt tccggcccta tcgagctgga
    12181 cctggacctg atcgacgaag atccgcatca gccgcggacg gccgacaacc ccggcttttc
    12241 cccggagagc atcgcggaaa tcggtgccac gatcaaagag cgcggggtga agtcacccat
    12301 ttcggtgcgc gagaaccagg agcagccggg ccgctatatc atcaatcacg gcgcccgccg
    12361 ctaccgtggc tcgaagtggg ccggcaagaa gtccatcccg gcgttcatcg acaacgacta
    12421 caacgaagcc gaccaggtta tcgagaacct gcaacgcaac gagctgaccc cgcgcgaaat
    12481 tgccgacttc attggccgcg agctggcgaa gggcaagaag aaaggcgata tcgccaagga
    12541 aatcggcaag tcgccggcgt tcatcaccca gcacgtcacg ctgctggacc tgccggagaa
    12601 gatcgccgat gcgttcaaca ccggccgcgt gcgcgacgtg accgtggtga acgagctggt
    12661 gacggccttc aagaagcgcc cggaggaagt cgaggcgtgg cttgacgacg acacccagga
    12721 aatcacgcgc ggcacggtca agctgctgcg cgagttcctg gacgagaagg gccgcgatcc
    12781 caacaccgtc gatgccttca acggccagac tgatgccgag cgtgacgcgg aggccggcga
    12841 cggccaggac ggcgaggacg gcgaccagga cggtaaggac gccaaggaaa agggcgcgaa
    12901 ggagccggac ccggacaagc tgaaaaaggc catcgtccag gtcgagcacg acgagcgccc
    12961 tgcccgcctt atcctcaacc gtcggccgcc ggcggaaggc tatgcctggt tgaagtacga
    13021 ggacgacggc caggagttcg aggcgaacct tgccgacgtg aaactggtcg cgctcatcga
    13081 gggctgatcc ccaaagacag cggcgcgggc cacccgcgcc gcacagacaa cggttccgct
    13141 acaaggagga ccgaagaatg aatccgatgc tgttctacat cgcgggaggc gtaggcgcgg
    13201 cgttgctgct ggtttccgcg atcatgctgt tcaagctgcg cgagccgaag aaggaacacc
    13261 gaccgcagcg caaggcggcg gccccgacgc cgcagccggt cgataacgag ctgctgcgca
    13321 cg
//
