LOCUS       sequence-265939-        6174 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of mouse Etv3 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark 
            NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, 
            Lemischka IR, Moore K
  TITLE     Induction of a hemogenic program in mouse fibroblasts.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 
            10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.
  PUBMED    23770078
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.05.024"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "205-18"
COMMENT     Sequence Label: pMXs-Etv3
FEATURES             Location/Qualifiers
     source          1..6174
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             28..621
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    684..1041
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1100..1516
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1446..1468
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1484..1500
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1526..1900
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1838..1854
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             3685..4154
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4284..4301)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4455..5043)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4535..4554)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5207..5998,5999..6067))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5830..5849
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6068..6172)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 catttccccg aaaagtgcca cctgcataat gaaagacccc acctgtaggt ttggcaagct
       61 agcttaagta acgccatttt gcaaggcatg gaaaaataca taactgagaa tagaaaagtt
      121 cagatcaagg tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
      181 aagcagttcc tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa
      241 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca
      301 gatgcggtcc agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa
      361 ggacctgaaa tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg
      421 ttcgcgcgct tctgctcccc gagctcaata aaagagccca caacccctca ctcggcgcgc
      481 cagtcctccg attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt
      541 tgcatccgac ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc
      601 cgtcagcggg ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga
      661 ccaccgaccc accaccggga ggtaagctgg ccagcaactt atctgtgtct gtccgattgt
      721 ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa ctagctctgt
      781 atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa ccctgggaga
      841 cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtccaaaa atcccgatcg
      901 ttttggactc tttggtgcac ccccctaata ggagggatat gtggttctgg taggagacga
      961 gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg accgaagccg
     1021 cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct
     1081 gtatttgtct gaaaattagg gccagactgt taccactccc ttaagtttga ccttaggtca
     1141 ctggaaagat gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg
     1201 ggttaccttc tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac
     1261 ctttaaccga gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg
     1321 acacccagac caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc
     1381 ctgggtcaag ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc
     1441 tctccccctt gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac
     1501 tccttctcta ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct
     1561 tgtaaacttc cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca
     1621 cttacaggct ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca
     1681 agaacaactg gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt
     1741 ccgccgacac cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct
     1801 gaccaccccc accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt
     1861 gaaggctgcc gaccccgggg gtggaccatc ctctagactg ccggatctag ctagttaatt
     1921 aaggatctcg agctcaagct tcgaattcat gaaagcaggc tgtagcatcg tggaaaagcc
     1981 agaaggaggt ggagggtatc agtttccgga ttgggcctac aaagccgagt cgtcgccggg
     2041 ctcccggcag atccagctgt ggcacttcat cctggaactg ctgcagaagg aagagttccg
     2101 ccatgtcatc gcctggcagc agggagagta cggggagttt gtcatcaagg atccagatga
     2161 agtggctcgc ctctggggcc gcaggaagtg caaaccacag atgaactatg acaagctgag
     2221 ccgggccctc agatactatt acaacaagag gatccttcat aaaacaaaag ggaaaaggtt
     2281 tacttacaag tttaacttca acaagctcgt gatgcccaac tacccgttca tcaacattcg
     2341 ctcgagtggt gtggttcctc agagtgcgcc accagtgcca acagcctcct cccggttcca
     2401 tttcccacct ctagacagcc attctcctac tggcgatgtg caaccgggtc gcttctctgc
     2461 cagctcactg agtgcttctg gccctgagtc aggggttacc actgacagga aggtcgagcc
     2521 ttcggacctg gaagatggct cggcctctga ctggcaccgg ggcatggact tcatgccctc
     2581 ccgaaatgct ctgggcggag gagcagtcgg ccaccagaaa cgcaagcctg acatactgct
     2641 tcctctcttc acccggccag ccatgtaccc tgacccgcac agtcccttcg ctatctctcc
     2701 ggtccctggc cgcggagggg tccttaatgt ccccatctca ccagccctgt ccctgactcc
     2761 caccatgttc tcctacagtc cctcaccagg cctgagcccc ttcaccagca gcagttgctt
     2821 ctccttcaac ccagaggaaa tgaaacacta ccttcattct caagcctgtt cggtgttcaa
     2881 ctaccatctg agtccccgga ctttcccccg ttacccaggg ctcatggtcc caccgctgca
     2941 gtgccaaatg catcctgagg agccttccca gttctctatc aagctgcagc ccccgccggc
     3001 tggacggaag aaccgcgaga gggtagagag ccgggaggag gctgttcgcg gctctgtgcc
     3061 cgccagcgct cctgttccct ctcggattaa ggtagagccg gccacagaga aggatcctga
     3121 cagcctccgg cagtcaaccc agggaaagga ggaacagacc caagaagtgg acagcattcg
     3181 gagcaggacc atagaagagg ggaaaggcac cgggtttgcc cacccctcac ccacctggcc
     3241 ctctgtgtcc attagcactc ccagtgacga acccctagag gggaccgaag acagtgagga
     3301 caggtctgcc agggagcccg gtgtacccga gaagaaagaa gacgccctga tgccccctaa
     3361 gcttcggctg aagaggcggt ggaatgacga ccctgaagcc agggagctca acaaaacggg
     3421 caagttcctc tggaatgggg caggacccca gggcctggcc acaacggcca ctgctgccgc
     3481 tgatgcttaa gttaacgcgg ccgccagcac agtggtcgac gataaaataa aagattttat
     3541 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     3601 aagtaacgcc attttgcaag gcatggaaaa atacataact gagaatagag aagttcagat
     3661 caaggtcagg aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     3721 gttcctgccc cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga
     3781 tatctgtggt aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc
     3841 ggtccagccc tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc
     3901 tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc
     3961 gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg ggcgccagtc
     4021 ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat
     4081 ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca
     4141 gcgggggtct ttcacatgca gcatgtatca aaattaattt ggtttttttt cttaagtatt
     4201 tacattaaat ggccatagtt gcattaatga atcggccaac gcgcggggag aggcggtttg
     4261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     4981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5041 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5101 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttgcggccg
     5161 gccgcaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     5221 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     5281 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     5341 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     5401 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     5461 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     5521 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     5581 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     5641 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     5701 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     5761 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     5821 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5881 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5941 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     6001 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     6061 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     6121 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgca
//
