LOCUS       sequence-265941-        9666 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of CSF1-R mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9666)
  AUTHORS   Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ
  TITLE     Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
  JOURNAL   Nature. 1987 Feb 5-11;325(6104):549-52.
  PUBMED    3027579
REFERENCE   2  (bases 1 to 9666)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1987 Feb 5-11"; volume: "325(6104)"; pages: "549-52"
COMMENT     Sequence Label: MSCV-human CSFI-R-L301S-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9666
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(4674..5248,5249..5251,5252..5260)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4841..4858)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5068..5087
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5261..5263,5264..5266,5267..5980)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5261..5263,5264..5266,5267..5977)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5306..5327)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5567..5586)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5914..5935
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6059..6573
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6735..6751
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6759..6765,6766..6783,6784..6789))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6804..6825
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6942..6959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7113..7701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7193..7212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7872..8663,8664..8732))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8495..8514
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8733..8837)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8905..8923
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8961..8983)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9083..9102
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9296..9318
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9446..9465
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9665..9666,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatccgc ggccgctgat
     1441 ccgaattcgc atggtcgact accagaggag aggaacgtgt gtccagtgtc ccgatccctg
     1501 cggagctagt agctgagagc tctgtgccct gggcaccttg cagccctgca cctgcctgcc
     1561 acttccccac cgaggccatg ggcccaggag ttctgctgct cctgctggtg gccacagctt
     1621 ggcatggtca gggaatccca gtgatagagc ccagtgtccc cgagctggtc gtgaagccag
     1681 gagcaacggt gaccttgcga tgtgtgggca atggcagcgt ggaatgggat ggccccccat
     1741 cacctcactg gaccctgtac tctgatggct ccagcagcat cctcagcacc aacaacgcta
     1801 ccttccaaaa cacggggacc tatcgctgca ctgagcctgg agaccccctg ggaggcagcg
     1861 ccgccatcca cctctatgtc aaagaccctg cccggccctg gaacgtgcta gcacaggagg
     1921 tggtcgtgtt cgaggaccag gacgcactac tgccctgtct gctcacagac ccggtgctgg
     1981 aagcaggcgt ctcgctggtg cgtgtgcgtg gccggcccct catgcgccac accaactact
     2041 ccttctcgcc ctggcatggc ttcaccatcc acagggccaa gttcattcag agccaggact
     2101 atcaatgcag tgccctgatg ggtggcagga aggtgatgtc catcagcatc cggctgaaag
     2161 tgcagaaagt catcccaggg cccccagcct tgacactggt gcctgcagag ctggtgcgga
     2221 ttcgagggga ggctgcccag atcgtgtgct cagccagcag cgttgatgtt aactttgatg
     2281 tcttcctcca acacaacaac actaagctcg caatccctca acaatctgac tttcataata
     2341 accgttacca aaaagtcctg accctcaacc tcgatcaagt agatttccaa catgccggca
     2401 actactcctg cgtggccagc aacgtgcagg gcaagcactc cacctccatg ttcttccggg
     2461 tggtagagag tgcctactcg aacttgagct ctgagcagaa cctcatccag gaggtgaccg
     2521 tgggggaggg gctcaacctc aaagtcatgg tggaggccta cccaggcctg caaggtttta
     2581 actggaccta cctgggaccc ttttctgacc accagcctga gcccaagctt gctaatgcta
     2641 ccaccaagga cacatacagg cacaccttca ccctctctct gccccgcctg aagccctctg
     2701 aggctggccg ctactccttc ctggccagaa acccaggagg ctggagagct ctgacgtttg
     2761 agctcaccct tcgatacccc ccagaggtaa gcgtcatatg gacattcatc aacggctctg
     2821 gcaccctttt gtgtgctgcc tctgggtacc cccagcccaa cgtgacatgg ctgcagtgca
     2881 gtggccacac tgataggtgt gatgaggccc aagtgctgca ggtctgggat gacccatacc
     2941 ctgaggtcct gagccaggag cccttccaca aggtgacggt gcagagcctg ctgactgttg
     3001 agaccttaga gcacaaccaa acctacgagt gcagggccca caacagcgtg gggagtggct
     3061 cctgggcctt catacccatc tctgcaggag cccacacgca tcccccggat gagttcctct
     3121 tcacaccagt ggtggtcgcc tgcatgtcca tcatggcctt gctgctgctg ctgctcctgc
     3181 tgctattgta caagtataag cagaagccca agtaccaggt ccgctggaag atcatcgaga
     3241 gctatgaggg caacagttat actttcatcg accccacgca gctgccttac aacgagaagt
     3301 gggagttccc ccggaacaac ctgcagtttg gtaagaccct cggagctgga gcctttggga
     3361 aggtggtgga ggccacggcc tttggtctgg gcaaggagga tgctgtcctg aaggtggctg
     3421 tgaagatgct gaagtccacg gcccatgctg atgagaagga ggccctcatg tccgagctga
     3481 agatcatgag ccacctgggc cagcacgaga acatcgtcaa ccttctggga gcctgtaccc
     3541 atggaggccc tgtactggtc atcacggagt actgttgcta tggcgacctg ctcaactttc
     3601 tgcgaaggaa ggctgaggcc atgctgggac ccagcctgag ccccggccag gaccccgagg
     3661 gaggcgtcga ctataagaac atccacctcg agaagaaata tgtccgcagg gacagtggct
     3721 tctccagcca gggtgtggac acctatgtgg agatgaggcc tgtctccact tcttcaaatg
     3781 actccttctc tgagcaagac ctggacaagg aggatggacg gcccctggag ctccgggacc
     3841 tgcttcactt ctccagccaa gtagcccagg gcatggcctt cctcgcttcc aagaattgca
     3901 tccaccggga cgtggcagcg cgtaacgtgc tgttgaccaa tggtcatgtg gccaagattg
     3961 gggacttcgg gctggctagg gacatcatga atgactccaa ctacattgtc aagggcaatg
     4021 cccgcctgcc tgtgaagtgg atggccccag agagcatctt tgactgtgtc tacacggttc
     4081 agagcgacgt ctggtcctat ggcatcctcc tctgggagat cttctcactt gggctgaatc
     4141 cctaccctgg catcctggtg aacagcaagt tctataaact ggtgaaggat ggataccaaa
     4201 tggcccagcc tgcatttgcc ccaaagaata tatacagcat catgcaggcc tgctgggcct
     4261 tggagcccac ccacagaccc accttccagc agatctgctc cttccttcag gagcaggccc
     4321 aagaggacag gagagagcgg gactatacca atctgccgag cagcagcaga agcggtggca
     4381 gcggcagcag cagcagtgag ctggaggagg agagctctag tgagcacctg acctgctgcg
     4441 agcaagggga tatcgcccag cccttgctgc agcccaacaa ctatcagttc tgctgaggag
     4501 ttgacgacag ggagtaccac tctcccctcc tccaaacttc aactcctcca tggatggggc
     4561 gacacgggga gaacatacaa actctgcctt cggtcatttc actcaacagc tcggcccagc
     4621 tctgaaactt gggaaggtga gggattcagg ggaggtcaga ggatcagcgg ccgcccctct
     4681 ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt
     4741 gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct
     4801 ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa
     4861 ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg
     4921 tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc
     4981 caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg
     5041 agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg
     5101 aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc
     5161 tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg
     5221 gttttccttt gaaaaacacg atgataatat ggccacaacc atggtgagca agggcgagga
     5281 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
     5341 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
     5401 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
     5461 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
     5521 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
     5581 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
     5641 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
     5701 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
     5761 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
     5821 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc
     5881 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     5941 cgccgggatc actctcggca tggacgagct gtacaagtaa ctcgagggat ccgtcgacct
     6001 gcagccaagc ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     6061 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     6121 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
     6181 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     6241 cagatggtcc ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc
     6301 cagggtgccc caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg
     6361 cttctcgctt ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc
     6421 tcactcggcg cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa
     6481 accctcttgc agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga
     6541 gtgattgact acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa
     6601 caacattctg agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt
     6661 gaatccacat actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg
     6721 ggagaattgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     6781 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     6841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     6901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     6961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     7021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     7081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     7141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     7201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     7261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     7321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     7381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     7441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     7501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     7561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     7621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     7681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     7741 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     7801 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     7861 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     7921 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     7981 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8041 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8101 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8161 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8221 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8281 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8341 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8401 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8461 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     8521 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     8581 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     8641 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     8701 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     8761 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     8821 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     8881 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     8941 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     9001 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     9061 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     9121 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     9181 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     9241 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     9301 tcacgacgtt gtaaaacgac ggcgcaagga atggtgcatg caaggagatg gcgcccaaca
     9361 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     9421 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     9481 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggcgatta gtccaatttg
     9541 ttaaagacag gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga
     9601 agcctataga gtacgagcca tagataaaat aaaagatttt atttagtctc cagaaaaagg
     9661 ggggaa
//
