LOCUS       sequence-265945-        9691 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  retroviral expression of CSF1-R mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9691)
  AUTHORS   Roussel MF, Dull TJ, Rettenmier CW, Ralph P, Ullrich A, Sherr CJ
  TITLE     Transforming potential of the c-fms proto-oncogene (CSF-1 receptor).
  JOURNAL   Nature. 1987 Feb 5-11;325(6104):549-52.
  PUBMED    3027579
REFERENCE   2  (bases 1 to 9691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            1987 Feb 5-11"; volume: "325(6104)"; pages: "549-52"
COMMENT     Sequence Label: MSCV-human CSFI-R-L301S- Y969F-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    join(4699..5273,5274..5276,5277..5285)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4866..4883)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5093..5112
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5286..5288,5289..5291,5292..6005)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5286..5288,5289..5291,5292..6002)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5331..5352)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5592..5611)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5939..5960
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6084..6598
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    6760..6776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6784..6790,6791..6808,6809..6814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6829..6850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6967..6984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7138..7726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7218..7237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7897..8688,8689..8757))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8520..8539
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8758..8862)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8930..8948
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8986..9008)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9108..9127
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9321..9343
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9471..9490
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9690..9691,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaattc gttaacctcg agagatccgc ggccgctgat
     1441 ccgctagagt cgacctgcag cccaagctaa ttcgcatggt cgactaccag aggagaggaa
     1501 cgtgtgtcca gtgtcccgat ccctgcggag ctagtagctg agagctctgt gccctgggca
     1561 ccttgcagcc ctgcacctgc ctgccacttc cccaccgagg ccatgggccc aggagttctg
     1621 ctgctcctgc tggtggccac agcttggcat ggtcagggaa tcccagtgat agagcccagt
     1681 gtccccgagc tggtcgtgaa gccaggagca acggtgacct tgcgatgtgt gggcaatggc
     1741 agcgtggaat gggatggccc cccatcacct cactggaccc tgtactctga tggctccagc
     1801 agcatcctca gcaccaacaa cgctaccttc caaaacacgg ggacctatcg ctgcactgag
     1861 cctggagacc ccctgggagg cagcgccgcc atccacctct atgtcaaaga ccctgcccgg
     1921 ccctggaacg tgctagcaca ggaggtggtc gtgttcgagg accaggacgc actactgccc
     1981 tgtctgctca cagacccggt gctggaagca ggcgtctcgc tggtgcgtgt gcgtggccgg
     2041 cccctcatgc gccacaccaa ctactccttc tcgccctggc atggcttcac catccacagg
     2101 gccaagttca ttcagagcca ggactatcaa tgcagtgccc tgatgggtgg caggaaggtg
     2161 atgtccatca gcatccggct gaaagtgcag aaagtcatcc cagggccccc agccttgaca
     2221 ctggtgcctg cagagctggt gcggattcga ggggaggctg cccagatcgt gtgctcagcc
     2281 agcagcgttg atgttaactt tgatgtcttc ctccaacaca acaacactaa gctcgcaatc
     2341 cctcaacaat ctgactttca taataaccgt taccaaaaag tcctgaccct caacctcgat
     2401 caagtagatt tccaacatgc cggcaactac tcctgcgtgg ccagcaacgt gcagggcaag
     2461 cactccacct ccatgttctt ccgggtggta gagagtgcct actcgaactt gagctctgag
     2521 cagaacctca tccaggaggt gaccgtgggg gaggggctca acctcaaagt catggtggag
     2581 gcctacccag gcctgcaagg ttttaactgg acctacctgg gacccttttc tgaccaccag
     2641 cctgagccca agcttgctaa tgctaccacc aaggacacat acaggcacac cttcaccctc
     2701 tctctgcccc gcctgaagcc ctctgaggct ggccgctact ccttcctggc cagaaaccca
     2761 ggaggctgga gagctctgac gtttgagctc acccttcgat accccccaga ggtaagcgtc
     2821 atatggacat tcatcaacgg ctctggcacc cttttgtgtg ctgcctctgg gtacccccag
     2881 cccaacgtga catggctgca gtgcagtggc cacactgata ggtgtgatga ggcccaagtg
     2941 ctgcaggtct gggatgaccc ataccctgag gtcctgagcc aggagccctt ccacaaggtg
     3001 acggtgcaga gcctgctgac tgttgagacc ttagagcaca accaaaccta cgagtgcagg
     3061 gcccacaaca gcgtggggag tggctcctgg gccttcatac ccatctctgc aggagcccac
     3121 acgcatcccc cggatgagtt cctcttcaca ccagtggtgg tcgcctgcat gtccatcatg
     3181 gccttgctgc tgctgctgct cctgctgcta ttgtacaagt ataagcagaa gcccaagtac
     3241 caggtccgct ggaagatcat cgagagctat gagggcaaca gttatacttt catcgacccc
     3301 acgcagctgc cttacaacga gaagtgggag ttcccccgga acaacctgca gtttggtaag
     3361 accctcggag ctggagcctt tgggaaggtg gtggaggcca cggcctttgg tctgggcaag
     3421 gaggatgctg tcctgaaggt ggctgtgaag atgctgaagt ccacggccca tgctgatgag
     3481 aaggaggccc tcatgtccga gctgaagatc atgagccacc tgggccagca cgagaacatc
     3541 gtcaaccttc tgggagcctg tacccatgga ggccctgtac tggtcatcac ggagtactgt
     3601 tgctatggcg acctgctcaa ctttctgcga aggaaggctg aggccatgct gggacccagc
     3661 ctgagccccg gccaggaccc cgagggaggc gtcgactata agaacatcca cctcgagaag
     3721 aaatatgtcc gcagggacag tggcttctcc agccagggtg tggacaccta tgtggagatg
     3781 aggcctgtct ccacttcttc aaatgactcc ttctctgagc aagacctgga caaggaggat
     3841 ggacggcccc tggagctccg ggacctgctt cacttctcca gccaagtagc ccagggcatg
     3901 gccttcctcg cttccaagaa ttgcatccac cgggacgtgg cagcgcgtaa cgtgctgttg
     3961 accaatggtc atgtggccaa gattggggac ttcgggctgg ctagggacat catgaatgac
     4021 tccaactaca ttgtcaaggg caatgcccgc ctgcctgtga agtggatggc cccagagagc
     4081 atctttgact gtgtctacac ggttcagagc gacgtctggt cctatggcat cctcctctgg
     4141 gagatcttct cacttgggct gaatccctac cctggcatcc tggtgaacag caagttctat
     4201 aaactggtga aggatggata ccaaatggcc cagcctgcat ttgccccaaa gaatatatac
     4261 agcatcatgc aggcctgctg ggccttggag cccacccaca gacccacctt ccagcagatc
     4321 tgctccttcc ttcaggagca ggcccaagag gacaggagag agcgggacta taccaatctg
     4381 ccgagcagca gcagaagcgg tggcagcggc agcagcagca gtgagctgga ggaggagagc
     4441 tctagtgagc acctgacctg ctgcgagcaa ggggatatcg cccagccctt gctgcagccc
     4501 aacaacttcc agttctgctg aggagttgac gacagggagt accactctcc cctcctccaa
     4561 acttcaactc ctccatggat ggggcgacac ggggagaaca tacaaactct gccttcggtc
     4621 atttcactca acagctcggc ccagctctga aacttgggaa ggtgagggat tcaggggagg
     4681 tcagaggatc agcggccgcc cctctccctc ccccccccct aacgttactg gccgaagccg
     4741 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     4801 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     4861 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     4921 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     4981 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     5041 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     5101 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     5161 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     5221 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
     5281 caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     5341 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     5401 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     5461 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     5521 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     5581 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5641 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5701 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     5761 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     5821 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     5881 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     5941 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     6001 agtaactcga gggatccgtc gacctgcagc caagcttatc gataaaataa aagattttat
     6061 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     6121 aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc
     6181 aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt
     6241 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
     6301 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     6361 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     6421 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
     6481 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     6541 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg
     6601 gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa
     6661 tcctgactca attagccact gttttgaatc cacatactcc aatactcctg aaatagttca
     6721 ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc tcacaattcc
     6781 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6841 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6901 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7561 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7741 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7801 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7861 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7921 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7981 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     8041 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     8101 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     8161 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8221 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8281 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8341 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8401 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8461 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8521 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8581 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8641 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8701 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8761 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8821 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8881 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8941 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     9001 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     9061 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     9121 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     9181 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     9241 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     9301 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc aaggaatggt
     9361 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     9421 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     9481 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     9541 gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
     9601 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag
     9661 attttattta gtctccagaa aaagggggga a
//
