LOCUS       sequence-265951-        6930 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human GATA2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Pereira CF, Chang B, Qiu J, Niu X, Papatsenko D, Hendry CE, Clark 
            NR, Nomura-Kitabayashi A, Kovacic JC, Ma'ayan A, Schaniel C, 
            Lemischka IR, Moore K
  TITLE     Induction of a hemogenic program in mouse fibroblasts.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):205-18. doi: 
            10.1016/j.stem.2013.05.024. Epub 2013 Jun 13.
  PUBMED    23770078
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.05.024"; journalName: "Cell Stem Cell"; date: 
            "2013-08-1- 1"; volume: "13"; issue: "2"; pages: "205-18"
COMMENT     Sequence Label: pMXs-GATA2
FEATURES             Location/Qualifiers
     source          1..6930
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..594
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    657..1014
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1073..1489
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1419..1441
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1457..1473
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1499..1873
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1811..1827
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             4414..4883
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5013..5030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5184..5772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5264..5283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5936..6727,6728..6796))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6559..6578
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6797..6901)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
       61 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
      121 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
      181 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
      241 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
      301 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
      361 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
      421 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
      481 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
      541 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
      601 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
      661 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
      721 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
      781 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
      841 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
      901 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
      961 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     1021 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     1081 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1141 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1201 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1261 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1321 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1381 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1441 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1501 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1561 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1621 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     1681 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     1741 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     1801 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     1861 atcctctaga ctgccggatc tagctagtta attaaggatc tcgagctcaa gcttcgaatt
     1921 ccggggccgg agccgggagg gcccgaggcc gaggcgcact ctacccccag ctcctaccct
     1981 gtaagccccg ccagcctccg gacgtgctgt ccctgggccc gtcgccctcg gggctcccgc
     2041 cggaactcct tcactctcag aggccgagtc cctcccctcc ccacggctgc gtgtggccgt
     2101 tgccgtctgc acccagaccc tgagccgccg ccgccggcca tggaggtggc gcccgagcag
     2161 ccgcgctgga tggcgcaccc ggccgtgctg aatgcgcagc accccgactc acaccacccg
     2221 ggcctggcgc acaactacat ggaacccgcg cagctgctgc ctccagacga ggtggacgtc
     2281 ttcttcaatc acctcgactc gcagggcaac ccctactatg ccaaccccgc tcacgcgcgg
     2341 gcgcgcgtct cctacagccc cgcgcacgcc cgcctgaccg gaggccagat gtgccgccca
     2401 cacttgttgc acagcccggg tttgccctgg ctggacgggg gcaaagcagc cctctctgcc
     2461 gctgcggccc accaccacaa cccctggacc gtgagcccct tctccaagac gccactgcac
     2521 ccctcagctg ctggaggccc tggaggccca ctctctgtgt acccaggggc tgggggtggg
     2581 agcgggggag gcagcgggag ctcagtggcc tccctcaccc ctacagcagc ccactctggc
     2641 tcccaccttt tcggcttccc acccacgcca cccaaagaag tgtctcctga ccctagcacc
     2701 acgggggctg cgtctccagc ctcatcttcc gcggggggta gtgcagcccg aggagaggac
     2761 aaggacggcg tcaagtacca ggtgtcactg acggagagca tgaagatgga aagtggcagt
     2821 cccctgcgcc caggcctagc tactatgggc acccagcctg ctacacacca ccccatcccc
     2881 acctacccct cctatgtgcc ggcggctgcc cacgactaca gcagcggact cttccacccc
     2941 ggaggcttcc tggggggacc ggcctccagc ttcaccccta agcagcgcag caaggctcgt
     3001 tcctgttcag aaggccggga gtgtgtcaac tgtggggcca cagccacccc tctctggcgg
     3061 cgggacggca ccggccacta cctgtgcaat gcctgtggcc tctaccacaa gatgaatggg
     3121 cagaaccgac cactcatcaa gcccaagcga agactgtcgg ccgccagaag agccggcacc
     3181 tgttgtgcaa attgtcagac gacaaccacc accttatggc gccgaaacgc caacggggac
     3241 cctgtctgca acgcctgtgg cctctactac aagctgcaca atgttaacag gccactgacc
     3301 atgaagaagg aagggatcca gactcggaac cggaagatgt ccaacaagtc caagaagagc
     3361 aagaaagggg cggagtgctt cgaggagctg tcaaagtgca tgcaggagaa gtcatccccc
     3421 ttcagtgcag ctgccctggc tggacacatg gcacctgtgg gccacctccc gcccttcagc
     3481 cactccggac acatcctgcc cactccgacg cccatccacc cctcctccag cctctccttc
     3541 ggccaccccc acccgtccag catggtgacc gccatgggct agggaacaga tggacgtcga
     3601 ggaccgggca ctcccgggat gggtggacca aacccttagc agcccagcat ttcccgaagg
     3661 ccgacaccac tcctgccagc ccggctcggc ccagcacccc ctctcctgga gggcgcccag
     3721 cagcctgcca gcagttactg tgaatgttcc ccaccgctga gaggctgcct ccgcacctga
     3781 ccgctgccca ggtggggttt cctgcatgga cagttgtttg gagaacaaca aggacaactt
     3841 tatgtagaga aaaggagggg acgggacaga cgaaggcaac catttttaga aggaaaaagg
     3901 attaggcaaa aataatttat tttgctcttg tttctaacaa ggacttggag acttggtggt
     3961 ctgagctgtc ccaagtcctc cggttcttcc tcgggattgg cgggtccact tgccagggct
     4021 ctgggggcag atttgtgggg acctcagcct gcaccctctt ctcctctggc ttccctctct
     4081 gaaatagccg aactccaggc tgggctgagc caaagccaga gtggccacgg cccagggagg
     4141 gtgagctggt gcctgctttg acgggccagg ccctggaggg cagagacaat cacgggcggt
     4201 cctgcaggaa ttcggatccg ttaacgcggc cgccagcaca gtggtcgacg ataaaataaa
     4261 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     4321 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     4381 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     4441 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     4501 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     4561 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     4621 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     4681 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     4741 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     4801 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     4861 tacccgtcag cgggggtctt tcacatgcag catgtatcaa aattaatttg gttttttttc
     4921 ttaagtattt acattaaatg gccatagttg cattaatgaa tcggccaacg cgcggggaga
     4981 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5041 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5101 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5161 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5221 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5281 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     5341 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5401 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5461 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5521 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5581 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5641 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5701 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5761 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5821 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5881 ttgcggccgg ccgcaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5941 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     6001 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     6061 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     6121 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     6181 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     6241 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     6301 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     6361 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     6421 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     6481 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     6541 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     6601 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     6661 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     6721 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6781 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6841 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6901 gcacatttcc ccgaaaagtg ccacctgcat
//
