LOCUS       sequence-265953-        8795 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged ATR C-terminal fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A
  TITLE     Preferential binding of ATR protein to UV-damaged DNA.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.
  PUBMED    12011431
REFERENCE   2  (bases 1 to 8795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-05-14"; volume: "99(10)"; pages: 
            "6673-8"
COMMENT     Sequence Label: pcDNA4-ATR-C
FEATURES             Location/Qualifiers
     source          1..8795
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..821
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     866..885
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        866..884
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             935..958
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4721..4750
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4766..4783
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4806..4823)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4812..5036
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5082..5510
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5169..5188)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5379..5400
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5519..5539)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5524..5853
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5704..5839
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5766..5785
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        5901..5948
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5967..6341
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6471..6592
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6508..6527)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6562..6581
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6641..6657)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6641..6657)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6654..6676)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6665..6681
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6689..6695,6696..6713,6714..6719))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6734..6755
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6872..6889)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7043..7631)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7123..7142)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7802..8593,8594..8662))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8425..8444
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8663..8767)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
      841 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
      901 agttaagctt ggtaccgagc tcggatccag gatggactac aaggacgacg atgacaagga
      961 gctaacaaga gcttaccttg cgtatgctga taatagccga gctcaagatt cagctgccta
     1021 tgccattcag gagttgcttt ctatttatga ctgtagagag atggagacca acggcccagg
     1081 tcaccaattg tggaggagat ttcctgagca tgttcgggaa atactagaac ctcatctaaa
     1141 taccagatac aagagttctc agaagtcaac cgattggtct ggagtaaaga agccaattta
     1201 cttaagtaaa ttgggtagta actttgcaga atggtcagca tcttgggcag gttatcttat
     1261 tacaaaggtt cgacatgatc ttgccagtaa aattttcacc tgctgtagca ttatgatgaa
     1321 gcatgatttc aaagtgacca tctatcttct tccacatatt ctggtgtatg tcttactggg
     1381 ttgtaatcaa gaagatcagc aggaggttta tgcagaaatt atggcagttc taaagcatga
     1441 cgatcagcat accataaata cccaagacat tgcatctgat ctgtgtcaac tcagtacaca
     1501 gactgtgttc tccatgcttg accatctcac acagtgggca aggcacaaat ttcaggcact
     1561 gaaagctgag aaatgtccac acagcaaatc aaacagaaat aaggtagact caatggtatc
     1621 tactgtggat tatgaagact atcagagtgt aacccatttt ctagacctca taccccagga
     1681 tactctggca gtagcttcct ttcgctccaa agcatacaca cgagctgtaa tgcactttga
     1741 atcatttatt acagaaaaga agcaaaatat tcaggaacat cttggatttt tacagaaatt
     1801 gtatgctgct atgcatgaac ctgatggagt ggccggagtc agtgcaatta gaaaggcaga
     1861 accatctcta aaagaacaga tccttgaaca tgaaagcctt ggcttgctga gggatgccac
     1921 tgcttgttat gacagggcta ttcagctaga accagaccag atcattcatt atcatggtgt
     1981 agtaaagtcc atgttaggtc ttggtcagct gtctactgtt atcactcagg tgaatggagt
     2041 gcatgctaac aggtccgagt ggacagatga attaaacacg tacagagtgg aagcagcttg
     2101 gaaattgtca cagtgggatt tggtggaaaa ctatttggca gcagatggaa aatctacaac
     2161 atggagtgtc agactgggac agctattatt atcagccaaa aaaagagata tcacagcttt
     2221 ttatgactca ctgaaactag tgagagcaga acaaattgta cctctttcag ctgcaagctt
     2281 tgaaagaggc tcctaccaac gaggatatga atatattgtg agattgcaca tgttatgtga
     2341 gttggagcat agcatcaaac cacttttcca gcattctcca ggtgacagtt ctcaagaaga
     2401 ttctctaaac tgggtagctc gactagaaat gacccagaat tcctacagag ccaaggagcc
     2461 tatcctggct ctccggaggg ctttactaag cctcaacaaa agaccagatt acaatgaaat
     2521 ggttggagaa tgctggctgc agagtgccag ggtagctaga aaggctggtc accaccagac
     2581 agcctacaat gctctcctta atgcagggga atcacgactc gctgaactgt acgtggaaag
     2641 ggcaaagtgg ctctggtcca agggtgatgt tcaccaggca ctaattgttc ttcaaaaagg
     2701 tgttgaatta tgttttcctg aaaatgaaac cccacctgag ggtaagaaca tgttaatcca
     2761 tggtcgagct atgctactag tgggccgatt tatggaagaa acagctaact ttgaaagcaa
     2821 tgcaattatg aaaaaatata aggatgtgac cgcgtgcctg ccagaatggg aggatgggca
     2881 tttttacctt gccaagtact atgacaaatt gatgcccatg gtcacagaca acaaaatgga
     2941 aaagcaaggt gatctcatcc ggtatatagt tcttcatttt ggcagatctc tacaatatgg
     3001 aaatcagttc atatatcagt caatgccacg aatgttaact ctatggcttg attatggtac
     3061 aaaggcatat gaatgggaaa aagctggccg ctccgatcgt gtacaaatga ggaatgattt
     3121 gggtaaaata aacaaggtta tcacagagca tacaaactat ttagctccat atcaattttt
     3181 gactgctttt tcacaattga tctctcgaat ttgtcattct cacgatgaag tttttgttgt
     3241 cttgatggaa ataatagcca aagtatttct agcctatcct caacaagcaa tgtggatgat
     3301 gacagctgtg tcaaagtcat cttatcccat gcgtgtgaac agatgcaagg aaatcctcaa
     3361 taaagctatt catatgaaaa aatccttaga gaagtttgtt ggagatgcaa ctcgcctaac
     3421 agataagctt ctagaattgt gcaataaacc ggttgatgga agtagttcca cattaagcat
     3481 gagcactcat tttaaaatgc ttaaaaagct ggtagaagaa gcaacattta gtgaaatcct
     3541 cattcctcta caatcagtca tgatacctac acttccatca attctgggta cccatgctaa
     3601 ccatgctagc catgaaccat ttcctggaca ttgggcctat attgcagggt ttgatgatat
     3661 ggtggaaatt cttgcttctc ttcagaaacc aaagaagatt tctttaaaag gctcagatgg
     3721 aaagttctac atcatgatgt gtaagccaaa agatgacctg agaaaggatt gtagactaat
     3781 ggaattcaat tccttgatta ataagtgctt aagaaaagat gcagagtctc gtagaagaga
     3841 acttcatatt cgaacatatg cagttattcc actaaatgat gaatgtggga ttattgaatg
     3901 ggtgaacaac actgctggtt tgagacctat tctgaccaaa ctatataaag aaaagggagt
     3961 gtatatgaca ggaaaagaac ttcgccagtg tatgctacca aagtcagcag ctttatctga
     4021 aaaactcaaa gtattccgag aatttctcct gcccaggcat cctcctattt ttcatgagtg
     4081 gtttctgaga acattccctg atcctacatc atggtacagt agtagatcag cttactgccg
     4141 ttccactgca gtaatgtcaa tggttggtta tattctgggg cttggagacc gtcatggtga
     4201 aaatattctc tttgattctt tgactggtga atgcgtacat gtagatttca attgtctttt
     4261 caataaggga gaaacctttg aagttccaga aattgtgcca tttcgcctga ctcataatat
     4321 ggttaatgga atgggtccta tgggaacaga gggtcttttt cgaagagcat gtgaagttac
     4381 aatgaggctg atgcgtgatc agcgagagcc tttaatgagt gtcttaaaga cttttctaca
     4441 tgatcctctt gtggaatgga gtaaaccagt gaaaggacat tccaaagcgc cactgaatga
     4501 aactggagaa gttgtcaatg aaaaggccaa gacccatgtt cttgacattg agcagcgact
     4561 acaaggtgta atcaagactc gaaatagagt gacaggactg ccgttatcta ttgaaggaca
     4621 tgtgcattac cttatacagg aagctactga tgaaaactta ctatgccaga tgtatcttgg
     4681 ttggactcca tatatgtgac tcgagtctag agggcccttc gaacaaaaac tcatctcaga
     4741 agaggatctg aatatgcata ccggtcatca tcaccatcac cattgagttt aaacccgctg
     4801 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4861 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4921 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4981 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     5041 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     5101 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     5161 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     5221 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     5281 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     5341 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     5401 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5461 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5521 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5581 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5641 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5701 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5761 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5821 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5881 ttcggatctg atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat
     5941 aatacgacaa ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca
     6001 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     6061 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     6121 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     6181 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     6241 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     6301 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg
     6361 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
     6421 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta
     6481 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     6541 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     6601 gtataccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     6661 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     6721 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     6781 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     6841 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     6901 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     6961 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     7021 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     7081 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     7141 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     7201 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     7261 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7321 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7381 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7441 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7501 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7561 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7621 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     7681 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     7741 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     7801 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     7861 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     7921 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     7981 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8041 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8101 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8161 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8221 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8281 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8341 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8401 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8461 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8521 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8581 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     8641 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     8701 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8761 ttccgcgcac atttccccga aaagtgccac ctgac
//
