LOCUS       sequence-265957-        9281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged ATR N-terminal fragment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Unsal-Kacmaz K, Makhov AM, Griffith JD, Sancar A
  TITLE     Preferential binding of ATR protein to UV-damaged DNA.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 May 14;99(10):6673-8.
  PUBMED    12011431
REFERENCE   2  (bases 1 to 9281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2002-05-14"; volume: "99(10)"; pages: 
            "6673-8"
COMMENT     Sequence Label: pcDNA4-ATR-N
FEATURES             Location/Qualifiers
     source          1..9281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(34..53)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        225..604
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        605..808
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     759..779
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     853..872
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        853..871
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             922..945
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5194..5223
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5239..5256
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5279..5296)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5285..5509
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5555..5983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5642..5661)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5852..5873
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5992..6012)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5997..6326
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6177..6312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6239..6258
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6374..6421
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6440..6814
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6944..7065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6981..7000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7035..7054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7114..7130)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7114..7130)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7127..7149)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7138..7154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7162..7168,7169..7186,7187..7192))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7207..7228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7345..7362)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7516..8104)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7596..7615)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8275..9066,9067..9135))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8898..8917
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9136..9240)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
       61 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
      121 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
      181 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
      241 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
      301 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      361 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      421 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      481 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      541 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      601 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      661 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      721 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      781 acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca ctgcttactg
      841 gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt taagcttggt
      901 accgagctcg gatccaggat ggactacaag gacgacgatg acaaggggga acatggcctg
      961 gagctggctt ccatgatccc cgccctgcgg gagctgggca gtgccacacc agaggaatat
     1021 aatacagttg tacagaagcc aagacaaatt ctgtgtcaat tcattgaccg gatacttaca
     1081 gatgtaaatg ttgttgctgt agaacttgta aagaaaactg actctcagcc aacctccgtg
     1141 atgttgcttg atttcatcca gcatatcatg aaatcctccc cacttatgtt tgtaaatgtg
     1201 agtggaagcc atgaggccaa aggcagttgt attgaattca gtaattggat cataacgaga
     1261 cttctgcgga ttgcagcaac tccctcctgt catttgttac acaagaaaat ctgtgaagtc
     1321 atctgttcat tattatttct ttttaaaagc aagagtcctg ctatttttgg ggtcctcaca
     1381 aaagaattat tacaactttt tgaagacttg gtttacctcc atagaagaaa tgtgatgggt
     1441 catgctgtgg aatggccagt ggtcatgagc cgatttttaa gtcaattaga tgaacacatg
     1501 ggatatttac aatcagctcc tttgcagttg atgagtatgc aaaatttaga atttattgaa
     1561 gtcactttat taatggttct tactcgtatt attgcaattg tgttttttag aaggcaagaa
     1621 ctcttacttt ggcagatagg ttgtgttctg ctagagtatg gtagtccaaa aattaaatcc
     1681 ctagcaatta gctttttaac agaacttttt cagcttggag gactaccagc acaaccagct
     1741 agcacttttt tcagctcatt tttggaatta ttaaaacacc ttgtagaaat ggatactgac
     1801 caattgaaac tctatgaaga gccattatca aagctgataa agacactatt tccctttgaa
     1861 gcagaagctt atagaaatat tgaacctgtc tatttaaata tgctgctgga aaaactctgt
     1921 gtcatgtttg aagacggtgt gctcatgcgg cttaagtctg atttgctaaa agcagctttg
     1981 tgccatttac tgcagtattt ccttaaattt gtgccagctg ggtatgaatc tgctttacaa
     2041 gtcaggaagg tctatgtgag aaatatttgt aaagctcttt tggatgtgct tggaattgag
     2101 gtagatgcag agtacttgtt gggcccactt tatgcagctt tgaaaatgga aagtatggaa
     2161 atcattgagg agattcaatg ccaaactcaa caggaaaacc tcagcagtaa tagtgatgga
     2221 atatcaccca aaaggcgtcg tctcagctcg tctctaaacc cttctaaaag agcaccaaaa
     2281 cagactgagg aaattaaaca tgtggacatg aaccaaaaga gcatattatg gagtgcactg
     2341 aaacagaaag ctgaatccct tcagatttcc cttgaataca gtggcctaaa gaatcctgtt
     2401 attgagatgt tagaaggaat tgctgttgtc ttacaactga ctgctctgtg tactgttcat
     2461 tgttctcatc aaaacatgaa ctgccgtact ttcaaggact gtcaacataa atccaagaag
     2521 aaaccttctg tagtgataac ttggatgtca ttggattttt acacaaaagt gcttaagagc
     2581 tgtagaagtt tgttagaatc tgttcagaaa ctggacctgg aggcaaccat tgataaggtg
     2641 gtgaaaattt atgatgcttt gatttatatg caagtaaaca gttcatttga agatcatatc
     2701 ctggaagatt tatgtggtat gctctcactt ccatggattt attcccattc tgatgatggc
     2761 tgtttaaagt tgaccacatt tgccgctaat cttctaacat taagctgtag gatttcagat
     2821 agctattcac cacaggcaca atcacgatgt gtgtttcttc tgactctgtt tccaagaaga
     2881 atattccttg agtggagaac agcagtttac aactgggccc tgcagagctc ccatgaagta
     2941 atccgggcta gttgtgttag tggatttttt atcttattgc agcagcagaa ttcttgtaac
     3001 agagttccca agattcttat agataaagtc aaagatgatt ctgacattgt caagaaagaa
     3061 tttgcttcta tacttggtca acttgtctgt actcttcacg gcatgtttta tctgacaagt
     3121 tctttaacag aacctttctc tgaacacgga catgtggacc tcttctgtag gaacttgaaa
     3181 gccacttctc aacatgaatg ttcatcttct caactaaaag cttctgtctg caagccattc
     3241 cttttcctac tgaaaaaaaa aatacctagt ccagtaaaac ttgctttcat agataatcta
     3301 catcatcttt gtaagcatct tgattttaga gaagatgaaa cagatgtaaa agcagttctt
     3361 ggaactttat taaatttaat ggaagatcca gacaaagatg ttagagtggc ttttagtgga
     3421 aatatcaagc acatattgga atccttggac tctgaagatg gatttataaa ggagcttttt
     3481 gtcttaagaa tgaaggaagc atatacacat gcccaaatat caagaaataa tgagctgaag
     3541 gataccttga ttcttacaac aggggatatt ggaagggccg caaaaggaga tttggtacca
     3601 tttgcactct tacacttatt gcattgtttg ttatccaagt cagcatctgt ctctggagca
     3661 gcatacacag aaattagagc tctggttgca gctaaaagtg ttaaactgca aagttttttc
     3721 agccagtata agaaacccat ctgtcagttt ttggtagaat cccttcactc tagtcagatg
     3781 acagcacttc cgaatactcc atgccagaat gctgacgtgc gaaaacaaga tgtggctcac
     3841 cagagagaaa tggctttaaa tacgttgtct gaaattgcca acgttttcga ctttcctgat
     3901 cttaatcgtt ttcttactag gacattacaa gttctactac ctgatcttgc tgccaaagca
     3961 agccctgcag cttctgctct cattcgaact ttaggaaaac aattaaatgt caatcgtaga
     4021 gagattttaa taaacaactt caaatatatt ttttctcatt tggtctgttc ttgttccaaa
     4081 gatgaattag aacgtgccct tcattatctg aagaatgaaa cagaaattga actggggagc
     4141 ctgttgagac aagatttcca aggattgcat aatgaattat tgctgcgtat tggagaacac
     4201 tatcaacagg tttttaatgg tttgtcaata cttgcctcat ttgcatccag tgatgatcca
     4261 tatcagggcc cgagagatat catatcacct gaactgatgg ctgattattt acaacccaaa
     4321 ttgttgggca ttttggcttt ttttaacatg cagttactga gctctagtgt tggcattgaa
     4381 gataagaaaa tggccttgaa cagtttgatg tctttgatga agttaatggg acccaaacat
     4441 gtcagttctg tgagggtgaa gatgatgacc acactgagaa ctggccttcg attcaaggat
     4501 gattttcctg aattgtgttg cagagcttgg gactgctttg ttcgctgcct ggatcatgct
     4561 tgtctgggct cccttctcag tcatgtaata gtagctttgt tacctcttat acacatccag
     4621 cctaaagaaa ctgcagctat cttccactac ctcataattg aaaacaggga tgctgtgcaa
     4681 gattttcttc atgaaatata ttttttacct gatcatccag aattaaaaaa gataaaagcc
     4741 gttctccagg aatacagaaa ggagacctct gagagcactg atcttcagac aactcttcag
     4801 ctctctatga aggccattca acatgaaaat gtcgatgttc gtattcatgc tcttacaagc
     4861 ttgaaggaaa ccttgtataa aaatcaggaa aaactgataa agtatgcaac agacagtgaa
     4921 acagtagaac ctattatctc acagttggtg acagtgcttt tgaaaggttg ccaagatgca
     4981 aactctcaag ctcggttgct ctgtggggaa tgtttagggg aattgggggc gatagatcca
     5041 ggtcgattag atttctcaac aactgaaact caaggaaaag attttacatt tgtgactgga
     5101 gtagaagatt caagctttgc ctatggatta ttgatggagc taacaagagc ttaccttgcg
     5161 tatgctgatt gactcgagtc tagagggccc ttcgaacaaa aactcatctc agaagaggat
     5221 ctgaatatgc ataccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc
     5281 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5341 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5401 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5461 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5521 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc
     5581 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5641 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5701 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5761 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5821 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5881 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5941 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt
     6001 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     6061 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6121 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6181 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6241 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6301 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat
     6361 ctgatcagca cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga
     6421 caaggtgagg aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg
     6481 cgacgtcgcc ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt
     6541 ggaggacgac ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca
     6601 ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct
     6661 gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat
     6721 gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa
     6781 ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac
     6841 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6901 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6961 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7021 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     7081 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     7141 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     7201 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     7261 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     7321 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     7381 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     7441 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     7501 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     7561 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     7621 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     7681 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     7741 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     7801 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7861 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7921 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7981 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     8041 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     8101 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     8161 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     8221 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     8281 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     8341 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     8401 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     8461 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     8521 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     8581 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     8641 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     8701 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     8761 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     8821 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8881 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8941 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     9001 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     9061 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     9121 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     9181 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     9241 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg g
//
