LOCUS       sequence-265960-        1228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral transfer vector for expression of roGFP2 and HyPer Red.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Faber SC, McNabb NA, Ariel P, Aungst ER, McCullough SD
  TITLE     Exposure Effects Beyond the Epithelial Barrier: Trans-Epithelial 
            Induction of Oxidative Stress by Diesel Exhaust Particulates in Lung
            Fibroblasts in an Organotypic Human Airway Model.
  JOURNAL   Toxicol Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085.
  PUBMED    32525552
REFERENCE   2  (bases 1 to 1228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Toxicol 
            Sci. 2020 Jun 11. pii: 5856111. doi: 10.1093/toxsci/kfaa085."
COMMENT     Sequence Label: pLV-dfROX
FEATURES             Location/Qualifiers
     source          1..1228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    19..135
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      192..201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(198..200,201..203,204..914)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(243..264)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(504..523)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     851..872
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             924..977
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     gap             1129..1142
                     /estimated_length=14
     gap             1202..1215
                     /estimated_length=14
ORIGIN
        1 ctcgnnggta tcggttactt ttaaagaaaa ggggggattg gggggtacag tgcaggggaa
       61 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
      121 aaaattcaaa attttatcga tcacgagact agcctcgagg tttaaacgcg gatccgcgac
      181 gcgtgctagc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      241 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      301 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctccaccacc ggcaagctgc
      361 ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct
      421 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      481 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
      541 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      601 gcaacatcct ggggcacaag ctggagtaca actacaactg ccacaacgtc tatatcatgg
      661 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
      721 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
      781 tgctgcccga caaccactac ctgagcacct gctccgccct gagcaaagac cccaacgaga
      841 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
      901 acgagctgta caagggaagc ggagagggca gaggaagtct gctaacatgc ggtgacgtcg
      961 aggagaatcc tggacccatg gagatggcga gccagcnggc gagacgatgt ccggaccgct
     1021 gcacattggt ttgattccca cagttggacc gtacctgcta ccgcatntnt ccctatgctg
     1081 caccagacnt ttccnaagcn ggnaangnat ctgcatganc nnngacccnn nnnnnnnnnn
     1141 nntnnnngng nnaanctcna nnnnnnatcn nncnnnnnnn aannnnannc naancatncn
     1201 tnnnnnnnnn nnnnngaann nannnang
//
