LOCUS       sequence-265966-       17167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rabies vector pSAD-Delta-G expressing ChR2-mCherry for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Osakada F, Mori T, Cetin AH, Marshel JH, Virgen B, Callaway EM
  TITLE     New rabies virus variants for monitoring and manipulating activity 
            and gene expression in defined neural circuits.
  JOURNAL   Neuron. 2011 Aug 25;71(4):617-31.
  PUBMED    21867879
REFERENCE   2  (bases 1 to 17167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron."; 
            date: "2011-08-25"; volume: "71(4)"; pages: "617-31"
COMMENT     Sequence Label: pSADdeltaG-ChR2-mCherry
FEATURES             Location/Qualifiers
     source          1..17167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(46..65)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        237..616
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        617..820
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     771..791
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     865..884
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        865..883
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4328..4337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4334..5260
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVILIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             5279..5989
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5424..5442)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5692..5711
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             7724..7735
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(12760..12777)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12766..12990
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      13036..13464
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13123..13142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13333..13354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(13473..13493)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        13478..13807
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13658..13793
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13874..14668
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(13928..13947)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     14538..14557
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    14842..14963
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14879..14898)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14933..14952
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(15012..15028)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15012..15028)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15025..15047)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15036..15052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15060..15066,15067..15084,15085..15090))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15105..15126
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15243..15260)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15414..16002)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15494..15513)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(16173..16964,16965..17033))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16796..16815
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(17034..17138)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
       61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
      121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
      181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
      241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
      301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
      661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
      721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
      781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
      841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta
      901 gcgtttaaac gggccctcta gactcgagcg gccgccagtg tgatggatat ctgcagaatt
      961 cgccctttta agcgtctgat gagtccgtga ggacgaaacc cggagtcccg ggtcacgctt
     1021 aacaaccaga tcaaagaaaa aacagacatt gtcaattgca aagcaaaaat gtaacacccc
     1081 tacaatggat gccgacaaga ttgtattcaa agtcaataat caggtggtct ctttgaagcc
     1141 tgagattatc gtggatcaat atgagtacaa gtaccctgcc atcaaagatt tgaaaaagcc
     1201 ctgtataacc ctaggaaagg ctcccgattt aaataaagca tacaagtcag ttttgtcagg
     1261 catgagcgcc gccaaactta atcctgacga tgtatgttcc tatttggcag cggcaatgca
     1321 gttttttgag gggacatgtc cggaagactg gaccagctat ggaattgtga ttgcacgaaa
     1381 aggagataag atcaccccag gttctctggt ggagataaaa cgtactgatg tagaagggaa
     1441 ttgggctctg acaggaggca tggaactgac aagagacccc actgtccctg agcatgcgtc
     1501 cttagtcggt cttctcttga gtctgtatag gttgagcaaa atatccgggc aaaacactgg
     1561 taactataag acaaacattg cagacaggat agagcagatt tttgagacag ccccttttgt
     1621 taaaatcgtg gaacaccata ctctaatgac aactcacaaa atgtgtgcta attggagtac
     1681 tataccaaac ttcagatttt tggccggaac ctatgacatg tttttctccc ggattgagca
     1741 tctatattca gcaatcagag tgggcacagt tgtcactgct tatgaagact gttcaggact
     1801 ggtatcattt actgggttca taaaacaaat caatctcacc gctagagagg caatactata
     1861 tttcttccac aagaactttg aggaagagat aagaagaatg tttgagccag ggcaggagac
     1921 agctgttcct cactcttatt tcatccactt ccgttcacta ggcttgagtg ggaaatctcc
     1981 ttattcatca aatgctgttg gtcacgtgtt caatctcatt cactttgtag gatgctatat
     2041 gggtcaagtc agatccctaa atgcaacggt tattgctgca tgtgctcctc atgaaatgtc
     2101 tgttctaggg ggctatctgg gagaggaatt cttcgggaaa gggacatttg aaagaagatt
     2161 cttcagagat gagaaagaac ttcaagaata cgaggcggct gaactgacaa agactgacgt
     2221 agcactggca gatgatggaa ctgtcaactc tgacgacgag gactactttt caggtgaaac
     2281 cagaagtccg gaggctgttt atactcgaat catgatgaat ggaggtcgac taaagagatc
     2341 tcacatacgg agatatgtct cagtcagttc caatcatcaa gcccgtccaa actcattcgc
     2401 cgagtttcta aacaagacat attcgagtga ctcataagaa gttgaataac aaaatgccgg
     2461 aaatctacgg attgtgtata tccatcatga aaaaaactaa cacccctcct ttcgaaccat
     2521 cccaaacatg agcaagatct ttgtcaatcc tagtgctatt agagccggtc tggccgatct
     2581 tgagatggct gaagaaactg ttgatctgat caatagaaat atcgaagaca atcaggctca
     2641 tctccaaggg gaacccatag aggtggacaa tctccctgag gatatggggc gacttcacct
     2701 ggatgatgga aaatcgccca accatggtga gatagccaag gtgggagaag gcaagtatcg
     2761 agaggacttt cagatggatg aaggagagga tcctagcttc ctgttccagt catacctgga
     2821 aaatgttgga gtccaaatag tcagacaaat gaggtcagga gagagatttc tcaagatatg
     2881 gtcacagacc gtagaagaga ttatatccta tgtcgcggtc aactttccca accctccagg
     2941 aaagtcttca gaggataaat caacccagac tactggccga gagctcaaga aggagacaac
     3001 acccactcct tctcagagag aaagccaatc atcgaaagcc aggatggcgg ctcaaattgc
     3061 ttctggccct ccagcccttg aatggtcggc taccaatgaa gaggatgatc tatcagtgga
     3121 ggctgagatc gctcaccaga ttgcagaaag tttctccaaa aaatataagt ttccctctcg
     3181 atcctcaggg atactcttgt ataattttga gcaattgaaa atgaaccttg atgatatagt
     3241 taaagaggca aaaaatgtac caggtgtgac ccgtttagcc catgacgggt ccaaactccc
     3301 cctaagatgt gtactgggat gggtcgcttt ggccaactct aagaaattcc agttgttagt
     3361 cgaatccgac aagctgagta aaatcatgca agatgacttg aatcgctata catcttgcta
     3421 accgaacctc tcccctcagt ccctctagac aataaaatcc gagatgtccc aaagtcaaca
     3481 tgaaaaaaac aggcaacacc actgataaaa tgaacctcct acgtaagata gtgaaaaacc
     3541 gcagggacga ggacactcaa aaatcctctc ccgcgtcagc ccctctggat gacgatgact
     3601 tgtggcttcc accccctgaa tacgtcccgc tgaaagaact tacaggcaag aagaacatga
     3661 ggaacttttg tatcaacgga agggttaaag tgtgtagccc gaatggttac tcgttcagga
     3721 tcctgcggca cattctgaaa tcattcgacg agatatattc tgggaatcat aggatgatcg
     3781 ggttagtcaa agtggttatt ggactggctt tgtcaggatc tccagtccct gagggcctga
     3841 actgggtata caaattgagg agaaccttta tcttccagtg ggctgattcc aggggccctc
     3901 ttgaagggga ggagttggaa tactctcagg agatcacttg ggatgatgat actgagttcg
     3961 tcggattgca aataagagtg attgcaaaac agtgtcatat ccagggcaga gtctggtgta
     4021 tcaacatgaa cccgagagca tgtcaactat ggtctgacat gtctcttcag acacaaaggt
     4081 ccgaagagga caaagattcc tctctgcttc tagaataatc agattatatc ccgcaaattt
     4141 atcacttgtt tacctctgga ggagagaaca tatgggctca actccaaccc ttgggagcaa
     4201 tataacaaaa aacatgttat ggtgccatta aaccgctgca tttcatcaaa gtcaagttga
     4261 ttacctttac attttgatcc tcttggatgt gaaaaaaact attaacatcc ctcaaaggac
     4321 gggatccgcc accatggatt atggaggcgc cctgagtgcc gttgggcgcg agctgctatt
     4381 tgtaacgaac ccagtagtcg tcaatggctc tgtacttgtg cctgaggacc agtgttactg
     4441 cgcgggctgg attgagtcgc gtggcacaaa cggtgcccaa acggcgtcga acgtgctgca
     4501 atggcttgct gctggcttct ccatcctact gcttatgttt tacgcctacc aaacatggaa
     4561 gtcaacctgc ggctgggagg agatctatgt gtgcgctatc gagatggtca aggtgattct
     4621 cgagttcttc ttcgagttta agaacccgtc catgctgtat ctagccacag gccaccgcgt
     4681 ccagtggttg cgttacgccg agtggcttct cacctgcccg gtcattctca ttcacctgtc
     4741 aaacctgacg ggcttgtcca acgactacag caggcgcacc atgggtctgc ttgtgtctga
     4801 tattggcaca attgtgtggg gcgccacttc cgccatggcc accggatacg tcaaggtcat
     4861 cttcttctgc ctgggtctgt gttatggtgc taacacgttc tttcacgctg ccaaggccta
     4921 catcgagggt taccacaccg tgccgaaggg ccggtgtcgc caggtggtga ctggcatggc
     4981 ttggctcttc ttcgtatcat ggggtatgtt ccccatcctg ttcatcctcg gccccgaggg
     5041 cttcggcgtc ctgagcgtgt acggctccac cgtcggccac accatcattg acctgatgtc
     5101 gaagaactgc tggggtctgc tcggccacta cctgcgcgtg ctgatccacg agcatatcct
     5161 catccacggc gacattcgca agaccaccaa attgaacatt ggtggcactg agattgaggt
     5221 cgagacgctg gtggaggacg aggccgaggc tggcgcggtc aacaagggca ccggcaagat
     5281 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     5341 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     5401 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     5461 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     5521 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     5581 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     5641 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     5701 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     5761 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     5821 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     5881 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     5941 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgaccc
     6001 gctagctttt cagtcgagaa aaaaacatta gatcagaaga acaactggca acacttctca
     6061 acctgagact tacttcaaga tgctcgatcc tggagaggtc tatgatgacc ctattgaccc
     6121 aatcgagtta gaggctgaac ccagaggaac ccccattgtc cccaacatct tgaggaactc
     6181 tgactacaat ctcaactctc ctttgataga agatcctgct agactaatgt tagaatggtt
     6241 aaaaacaggg aatagacctt atcggatgac tctaacagac aattgctcca ggtctttcag
     6301 agttttgaaa gattatttca agaaggtaga tttgggttct ctcaaggtgg gcggaatggc
     6361 tgcacagtca atgatttctc tctggttata tggtgcccac tctgaatcca acaggagccg
     6421 gagatgtata acagacttgg cccatttcta ttccaagtcg tcccccatag agaagctgtt
     6481 gaatctcacg ctaggaaata gagggctgag aatcccccca gagggagtgt taagttgcct
     6541 tgagagggtt gattatgata atgcatttgg aaggtatctt gccaacacgt attcctctta
     6601 cttgttcttc catgtaatca ccttatacat gaacgcccta gactgggatg aagaaaagac
     6661 catcctagca ttatggaaag atttaacctc agtggacatc gggaaggact tggtaaagtt
     6721 caaagaccaa atatggggac tgctgatcgt gacaaaggac tttgtttact cccaaagttc
     6781 caattgtctt tttgacagaa actacacact tatgctaaaa gatcttttct tgtctcgctt
     6841 caactcctta atggtcttgc tctctccccc agagccccga tactcagatg acttgatatc
     6901 tcaactatgc cagctgtaca ttgctgggga tcaagtcttg tctatgtgtg gaaactccgg
     6961 ctatgaagtc atcaaaatat tggagccata tgtcgtgaat agtttagtcc agagagcaga
     7021 aaagtttagg cctctcattc attccttggg agactttcct gtatttataa aagacaaggt
     7081 aagtcaactt gaagagacgt tcggtccctg tgcaagaagg ttctttaggg ctctggatca
     7141 attcgacaac atacatgact tggtttttgt gtttggctgt tacaggcatt gggggcaccc
     7201 atatatagat tatcgaaagg gtctgtcaaa actatatgat caggttcacc ttaaaaaaat
     7261 gatagataag tcctaccagg agtgcttagc aagcgaccta gccaggagga tccttagatg
     7321 gggttttgat aagtactcca agtggtatct ggattcaaga ttcctagccc gagaccaccc
     7381 cttgactcct tatatcaaaa cccaaacatg gccacccaaa catattgtag acttggtggg
     7441 ggatacatgg cacaagctcc cgatcacgca gatctttgag attcctgaat caatggatcc
     7501 gtcagaaata ttggatgaca aatcacattc tttcaccaga acgagactag cttcttggct
     7561 gtcagaaaac cgaggggggc ctgttcctag cgaaaaagtt attatcacgg ccctgtctaa
     7621 gccgcctgtc aatccccgag agtttctgag gtctatagac ctcggaggat tgccagatga
     7681 agacttgata attggcctca agccaaagga acgggaattg aagattgaag gtcgattctt
     7741 tgctctaatg tcatggaatc taagattgta ttttgtcatc actgaaaaac tcttggccaa
     7801 ctacatcttg ccactttttg acgcgctgac tatgacagac aacctgaaca aggtgtttaa
     7861 aaagctgatc gacagggtca ccgggcaagg gcttttggac tattcaaggg tcacatatgc
     7921 atttcacctg gactatgaaa agtggaacaa ccatcaaaga ttagagtcaa cagaggatgt
     7981 attttctgtc ctagatcaag tgtttggatt gaagagagtg ttttctagaa cacacgagtt
     8041 ttttcaaaag gcctggatct attattcaga cagatcagac ctcatcgggt tacgggagga
     8101 tcaaatatac tgcttagatg cgtccaacgg cccaacctgt tggaatggcc aggatggcgg
     8161 gctagaaggc ttacggcaga agggctggag tctagtcagc ttattgatga tagatagaga
     8221 atctcaaatc aggaacacaa gaaccaaaat actagctcaa ggagacaacc aggttttatg
     8281 tccgacatac atgttgtcgc cagggctatc tcaagagggg ctcctctatg aattggagag
     8341 aatatcaagg aatgcacttt cgatatacag agccgtcgag gaaggggcat ctaagctagg
     8401 gctgatcatc aagaaagaag agaccatgtg tagttatgac ttcctcatct atggaaaaac
     8461 ccctttgttt agaggtaaca tattggtgcc tgagtccaaa agatgggcca gagtctcttg
     8521 cgtctctaat gaccaaatag tcaacctcgc caatataatg tcgacagtgt ccaccaatgc
     8581 gctaacagtg gcacaacact ctcaatcttt gatcaaaccg atgagggatt ttctgctcat
     8641 gtcagtacag gcagtctttc actacctgct atttagccca atcttaaagg gaagagttta
     8701 caagattctg agcgctgaag gggagagctt tctcctagcc atgtcaagga taatctatct
     8761 agatccttct ttgggaggga tatctggaat gtccctcgga agattccata tacgacagtt
     8821 ctcagaccct gtctctgaag ggttatcctt ctggagagag atctggttaa gctcccaaga
     8881 gtcctggatt cacgcgttgt gtcaagaggc tggaaaccca gatcttggag agagaacact
     8941 cgagagcttc actcgccttc tagaagatcc gaccacctta aatatcagag gaggggccag
     9001 tcctaccatt ctactcaagg atgcaatcag aaaggcttta tatgacgagg tggacaaggt
     9061 ggaaaattca gagtttcgag aggcaatcct gttgtccaag acccatagag ataattttat
     9121 actcttctta atatctgttg agcctctgtt tcctcgattt ctcagtgagc tattcagttc
     9181 gtcttttttg ggaatccccg agtcaatcat tggattgata caaaactccc gaacgataag
     9241 aaggcagttt agaaagagtc tctcaaaaac tttagaagaa tccttctaca actcagagat
     9301 ccacgggatt agtcggatga cccagacacc tcagagggtt gggggggtgt ggccttgctc
     9361 ttcagagagg gcagatctac ttagggagat ctcttgggga agaaaagtgg taggcacgac
     9421 agttcctcac ccttctgaga tgttgggatt acttcccaag tcctctattt cttgcacttg
     9481 tggagcaaca ggaggaggca atcctagagt ttctgtatca gtactcccgt cctttgatca
     9541 gtcatttttt tcacgaggcc ccctaaaggg atacttgggc tcgtccacct ctatgtcgac
     9601 ccagctattc catgcatggg aaaaagtcac taatgttcat gtggtgaaga gagctctatc
     9661 gttaaaagaa tctataaact ggttcattac tagagattcc aacttggctc aagctctaat
     9721 taggaacatt atgtctctga caggccctga tttccctcta gaggaggccc ctgtcttcaa
     9781 aaggacgggg tcagccttgc ataggttcaa gtctgccaga tacagcgaag gagggtattc
     9841 ttctgtctgc ccgaacctcc tctctcatat ttctgttagt acagacacca tgtctgattt
     9901 gacccaagac gggaagaact acgatttcat gttccagcca ttgatgcttt atgcacagac
     9961 atggacatca gagctggtac agagagacac aaggctaaga gactctacgt ttcattggca
    10021 cctccgatgc aacaggtgtg tgagacccat tgacgacgtg accctggaga cctctcagat
    10081 cttcgagttt ccggatgtgt cgaaaagaat atccagaatg gtttctgggg ctgtgcctca
    10141 cttccagagg cttcccgata tccgtctgag accaggagat tttgaatctc taagcggtag
    10201 agaaaagtct caccatatcg gatcagctca ggggctctta tactcaatct tagtggcaat
    10261 tcacgactca ggatacaatg atggaaccat cttccctgtc aacatatacg gcaaggtttc
    10321 ccctagagac tatttgagag ggctcgcaag gggagtattg ataggatcct cgatttgctt
    10381 cttgacaaga atgacaaata tcaatattaa tagacctctt gaattggtct caggggtaat
    10441 ctcatatatt ctcctgaggc tagataacca tccctccttg tacataatgc tcagagaacc
    10501 gtctcttaga ggagagatat tttctatccc tcagaaaatc cccgccgctt atccaaccac
    10561 tatgaaagaa ggcaacagat caatcttgtg ttatctccaa catgtgctac gctatgagcg
    10621 agagataatc acggcgtctc cagagaatga ctggctatgg atcttttcag actttagaag
    10681 tgccaaaatg acgtacctat ccctcattac ttaccagtct catcttctac tccagagggt
    10741 tgagagaaac ctatctaaga gtatgagaga taacctgcga caattgagtt ctttgatgag
    10801 gcaggtgctg ggcgggcacg gagaagatac cttagagtca gacgacaaca ttcaacgact
    10861 gctaaaagac tctttacgaa ggacaagatg ggtggatcaa gaggtgcgcc atgcagctag
    10921 aaccatgact ggagattaca gccccaacaa gaaggtgtcc cgtaaggtag gatgttcaga
    10981 atgggtctgc tctgctcaac aggttgcagt ctctacctca gcaaacccgg cccctgtctc
    11041 ggagcttgac ataagggccc tctctaagag gttccagaac cctttgatct cgggcttgag
    11101 agtggttcag tgggcaaccg gtgctcatta taagcttaag cctattctag atgatctcaa
    11161 tgttttccca tctctctgcc ttgtagttgg ggacgggtca ggggggatat caagggcagt
    11221 cctcaacatg tttccagatg ccaagcttgt gttcaacagt cttttagagg tgaatgacct
    11281 gatggcttcc ggaacacatc cactgcctcc ttcagcaatc atgaggggag gaaatgatat
    11341 cgtctccaga gtgatagatc ttgactcaat ctgggaaaaa ccgtccgact tgagaaactt
    11401 ggcaacctgg aaatacttcc agtcagtcca aaagcaggtc aacatgtcct atgacctcat
    11461 tatttgcgat gcagaagtta ctgacattgc atctatcaac cggatcaccc tgttaatgtc
    11521 cgattttgca ttgtctatag atggaccact ctatttggtc ttcaaaactt atgggactat
    11581 gctagtaaat ccaaactaca aggctattca acacctgtca agagcgttcc cctcggtcac
    11641 agggtttatc acccaagtaa cttcgtcttt ttcatctgag ctctacctcc gattctccaa
    11701 acgagggaag tttttcagag atgctgagta cttgacctct tccacccttc gagaaatgag
    11761 ccttgtgtta ttcaattgta gcagccccaa gagtgagatg cagagagctc gttccttgaa
    11821 ctatcaggat cttgtgagag gatttcctga agaaatcata tcaaatcctt acaatgagat
    11881 gatcataact ctgattgaca gtgatgtaga atcttttcta gtccacaaga tggttgatga
    11941 tcttgagtta cagaggggaa ctctgtctaa agtggctatc attatagcca tcatgatagt
    12001 tttctccaac agagtcttca acgtttccaa acccctaact gacccctcgt tctatccacc
    12061 gtctgatccc aaaatcctga ggcacttcaa catatgttgc agtactatga tgtatctatc
    12121 tactgcttta ggtgacgtcc ctagcttcgc aagacttcac gacctgtata acagacctat
    12181 aacttattac ttcagaaagc aagtcattcg agggaacgtt tatctatctt ggagttggtc
    12241 caacgacacc tcagtgttca aaagggtagc ctgtaattct agcctgagtc tgtcatctca
    12301 ctggatcagg ttgatttaca agatagtgaa gactaccaga ctcgttggca gcatcaagga
    12361 tctatccaga gaagtggaaa gacaccttca taggtacaac aggtggatca ccctagagga
    12421 tatcagatct agatcatccc tactagacta cagttgcctg tgaaccggat actcctggaa
    12481 gcctgcccat gctaagactc ttgtgtgatg tatcttgaaa aaaacaagat cctaaatctg
    12541 aacctttggt tgtttgattg tttttctcat ttttgttgtt tatttgttaa gcgtgggtcg
    12601 gcatggcatc tccacctcct cgcggtccga cctgggcatc cgaaggagga cgcacgtcca
    12661 ctcggatggc taagggagaa gggcgaattc cagcacactg gcggccgtta ctagtggatc
    12721 cgagctcggt accaagctta agtttaaacc gctgatcagc ctcgactgtg ccttctagtt
    12781 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    12841 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    12901 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    12961 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    13021 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    13081 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    13141 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    13201 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    13261 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    13321 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    13381 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    13441 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
    13501 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    13561 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    13621 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    13681 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    13741 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    13801 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag agacaggatg
    13861 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    13921 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    13981 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    14041 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    14101 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    14161 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    14221 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    14281 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    14341 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    14401 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    14461 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    14521 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    14581 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    14641 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    14701 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    14761 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    14821 ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc
    14881 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    14941 tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg
    15001 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    15061 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    15121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    15181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    15241 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    15301 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    15361 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    15421 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    15481 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    15541 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    15601 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    15661 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    15721 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    15781 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    15841 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    15901 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    15961 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    16021 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    16081 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    16141 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    16201 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    16261 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    16321 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    16381 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    16441 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    16501 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    16561 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    16621 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    16681 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    16741 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    16801 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    16861 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    16921 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    16981 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    17041 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    17101 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    17161 cctgacg
//
