LOCUS       sequence-266006-       10948 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express flag-USP19 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10948)
  AUTHORS   Lee JG, Takahama S, Zhang G, Tomarev SI, Ye Y
  TITLE     Unconventional secretion of misfolded proteins promotes adaptation 
            to proteasome dysfunction in mammalian cells.
  JOURNAL   Nat Cell Biol. 2016 Jul;18(7):765-76. doi: 10.1038/ncb3372. Epub 
            2016 Jun 13.
  PUBMED    27295555
REFERENCE   2  (bases 1 to 10948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/ncb3372"; 
            journalName: "Nat Cell Biol"; date: "2016-07"; volume: "18"; issue: 
            "7"; pages: "765-76"
COMMENT     Sequence Label: pRK-flag-USP19
FEATURES             Location/Qualifiers
     source          1..10948
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        815..833
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     regulatory      905..914
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             914..937
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(4938..4956)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4976..4993)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4982..5206
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5252..5680
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5694..6023
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5874..6009
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6090..6884
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6144..6163)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6754..6773
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7058..7179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      7235..7244
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7248..7382
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7451..7808
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7451..7642
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      7659..7794
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7828..7845)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7828..7844)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7837..7859)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8057..8512
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8529..8548)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8648..8670
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8708..8726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8794..8898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8899..8967,8968..9759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9117..9136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9930..10518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10419..10438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10672..10689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10806..10827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10842..10847,10848..10865,10866..10872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10880..10896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10885..10907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10904..10920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10904..10920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg
      721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca
      781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat
      841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctaa
      901 gcttgccgcc atggactaca aggacgacga tgacaagggg tcgaccatgt ctggcggggc
      961 cagtgccaca ggcccaagga gagggccccc aggactggag gacaccacta gtaagaagaa
     1021 gcagaaggat cgagcaaacc aggagagcaa ggatggagat cctaggaaag agacagggtc
     1081 tcgatatgtt gcccaggctg gtcttgaacc tctggcctca ggtgatcctt ctgcctcagc
     1141 ctcccatgca gctgggatca caggctcacg ccaccgtacc cggctgttct ttccttcatc
     1201 gtcagggtca gcatccactc ctcaagagga gcagaccaaa gagggagctt gtgaagaccc
     1261 tcatgatctc ttggctactc ccactccaga gttgttgctc gattggaggc agagtgcaga
     1321 agaggtgatt gtcaagcttc gtgtgggagt aggtcccctg cagctggagg atgtagatgc
     1381 tgctttcaca gatacagact gtgtggtgcg gtttgcaggt ggtcagcagt ggggtggtgt
     1441 cttctatgct gagataaaaa gctcttgtgc taaagtgcaa acccgcaagg gcagtctcct
     1501 gcacctgaca ctgcccaaaa aggtgcctat gctcacgtgg ccctccctcc tggttgaggc
     1561 tgatgaacag ctttgcatac caccgctgaa ctcccaaacc tgcctcctgg gctcagagga
     1621 gaatttagcc cctttggcag gagagaaagc agtgcctccc gggaatgacc cagtctctcc
     1681 agccatggtc cggagcagaa accctgggaa agatgactgt gccaaggagg agatggcagt
     1741 ggcagcagat gctgcaacct tggtggatga gcccgagtcg atggtgaacc tggcgtttgt
     1801 caagaatgac tcgtatgaga agggcccgga ttcagtggtg gtgcacgtgt acgtgaagga
     1861 gatctgcagg gacacctcaa gagtactttt ccgtgagcag gacttcacgc tcatcttcca
     1921 gaccagggat ggaaacttcc tgaggctgca cccgggctgt gggccccaca ccaccttccg
     1981 ttggcaggtg aagctcagga atctgattga gccagagcag tgcaccttct gtttcacggc
     2041 ttctcgcatc gacatctgcc ttcgtaagag gcagagtcag cgctgggggg gcctggaggc
     2101 cccggctgca cgagtgggtg gtgcaaaggt tgccgtgccg acaggtccaa cccctctgga
     2161 ttcaacccca ccaggaggtg ctccccaccc cctgacaggc caggaggagg cccgggctgt
     2221 ggagaaggat aaatccaagg cacgatctga ggacacaggg ctagacagtg tggcaacccg
     2281 cacacccatg gagcatgtaa ccccaaagcc agagacacac ctggcctcgc ccaagcctac
     2341 atgcatggtg cctcccatgc cccacagccc agttagtgga gacagcgtgg aggaggagga
     2401 agaggaagag aagaaggtgt gtctgccagg cttcactggc cttgtcaatt taggcaacac
     2461 ctgcttcatg aacagcgtca ttcagtctct gtccaacact cgggaactcc gggacttctt
     2521 ccatgaccgc tcctttgagg ctgagatcaa ctacaacaac ccactaggga ctggtgggcg
     2581 tctggccatt ggctttgccg tgctgcttcg ggcgctgtgg aagggcaccc accatgcctt
     2641 ccagccttcc aagttgaagg ccattgtggc gagtaaggcc agccagttca caggctatgc
     2701 acagcatgat gcccaggagt tcatggcttt cctgctggat gggctgcacg aggacctgaa
     2761 tcgcattcag aacaagccct acacagagac cgtggattca gatgggcggc ccgatgaggt
     2821 ggtagctgag gaagcatggc agcggcacaa gatgaggaat gactctttca tcgtggacct
     2881 atttcagggg cagtacaagt cgaagctggt gtgccctgtg tgtgccaagg tctccatcac
     2941 ttttgacccg tttctttatc tgccggtgcc cttgccacaa aagcaaaagg ttctccctgt
     3001 cttttatttt gcccgagagc cccacagcaa gcccatcaag ttcctggtga gcgtcagcaa
     3061 ggagaactcc actgcgagcg aagtattgga ctccctctct cagagtgttc atgtgaagcc
     3121 tgagaacctg cgtttggcgg aggtaattaa gaatcgtttt catcgtgtgt tcctaccctc
     3181 ccactcactg gacactgtgt ccccatctga tacgctcctc tgctttgagc tgctatcctc
     3241 agagttggct aaggagcggg tagtggtgct agaggtgcaa cagcgccccc aggtgcccag
     3301 cgtccccatc tccaagtgtg cagcctgcca gcggaagcaa cagtcggagg atgaaaagct
     3361 gaagcgctgt acccggtgct accgtgtggg ctactgcaac cagctctgcc agaaaaccca
     3421 ctggcctgac cacaagggcc tctgccgacc tgagaacatt ggctacccct tcctggtcag
     3481 tgtacctgcc tcacgcctca cttatgcccg cctcgctcag ttgctagagg gctatgcccg
     3541 gtactctgtg agtgtattcc agccaccctt tcagccaggc cgcatggcct tggagtctca
     3601 gagccctggc tgcaccacac tgctctccac aggttccctg gaggctgggg acagcgagag
     3661 agaccccatt cagccacctg agctccagct ggtgacccct atggctgagg gggacacagg
     3721 gcttccccgg gtgtgggcag cccctgaccg gggtcctgtg cccagcacca gtggaatttc
     3781 ttctgagatg ctggccagtg ggcccattga ggttggctcc ttgccagctg gcgagagggt
     3841 gtcccgaccc gaagctgctg tgcctgggta ccagcatcca agtgaagcta tgaatgccca
     3901 cacaccccag ttcttcatct ataaaattga ttcatccaac cgagagcagc ggctagagga
     3961 caaaggagac accccactgg agctgggtga cgactgtagc ctggctctcg tctggcggaa
     4021 caatgagcgc ttgcaggagt ttgtgttggt agcctccaag gagctggaat gtgctgagga
     4081 tccaggctct gccggtgagg ctgcccgggc cggccacttc accctggacc agtgcctcaa
     4141 cctcttcaca cggcctgagg tgctggcacc cgaggaggcc tggtactgcc cacagtgcaa
     4201 acagcaccgt gaggcctcca agcagctgtt gctatggcgc ctgccaaatg ttctcatcgt
     4261 gcagctcaag cgcttctcct ttcgtagttt tatctggcgt gacaagatca atgacttggt
     4321 ggagttccct gttaggaacc tggacctgag caagttctgc attggtcaga aagaggagca
     4381 gctgcccagc tacgatctat atgctgtcat caaccactat ggaggcatga ttggtggcca
     4441 ctacactgcc tgtgcacgcc tgcccaatga tcgtagcagt cagcgcagtg acgtgggctg
     4501 gcgcttgttt gatgacagca cagtgacaac ggtagacgag agccaggttg tgacgcgtta
     4561 tgcctatgta ctcttctacc gccggcggaa ctctcctgtg gagaggcccc ccagggcggg
     4621 tcactctgag caccacccag acctaggccc tgcagctgag gctgctgcca gccaggcttc
     4681 ccggatttgg caggagctgg aggctgagga ggagccggtg cctgaggggt ctgggcccct
     4741 gggtccctgg gggccccaag actgggtggg ccccctacca cgtggcccta ccacaccaga
     4801 tgagggctgc ctccggtact ttgtcctggg caccgtggcg gctttggtgg ccctcgtgct
     4861 caacgtgttc tatcctctgg tatcccagag tcgctggaga taagcggccg ctcgagcatg
     4921 catctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     4981 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5041 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5101 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5161 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5221 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5281 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5341 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5401 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5461 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5521 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5581 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5641 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5701 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5761 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5821 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     5881 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     5941 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6001 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6061 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     6121 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     6181 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     6241 ccgacctgtc cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg
     6301 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     6361 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     6421 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     6481 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     6541 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     6601 tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     6661 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     6721 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     6781 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     6841 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     6901 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     6961 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     7021 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     7081 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     7141 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgag
     7201 gatccccggg taccgagctc gaattcaatc gatggccgcc atggcccaac ttgtttattg
     7261 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     7321 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     7381 tcgggaatta attcggcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt
     7441 taggtacctt ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
     7501 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     7561 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     7621 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7681 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7741 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7801 tttgcaaaaa gctgttaaca gcttggcact ggccgtcgtt ttacaacgtc gtgactggga
     7861 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
     7921 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
     7981 atggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg
     8041 tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     8101 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     8161 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     8221 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat
     8281 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     8341 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc
     8401 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     8461 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc
     8521 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     8581 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8641 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     8701 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8761 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     8941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     9961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10681 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
    10801 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
    10861 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
    10921 atgattacga attaattcga gctcgccc
//
