LOCUS       sequence-266007-        9589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-ICP0 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM
  TITLE     Single-cell analysis of Daxx and ATRX-dependent transcriptional 
            repression.
  JOURNAL   J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 
            10.1242/jcs.110148. Epub 2012 Sep 12.
  PUBMED    22976303
REFERENCE   2  (bases 1 to 9589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-11-15"; volume: "125(Pt 22):5489-501. doi"; 
            pages: " 10.1242/jcs.110148. Epub 2012 Sep 12"
COMMENT     Sequence Label: pLU-YFP-ICP0
FEATURES             Location/Qualifiers
     source          1..9589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    619..852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1037..1081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1348..1464
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1487..1790
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1791..1994
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1945..1965
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1991..2015
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2081..2090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2087..2089,2090..2092,2093..2803)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(2132..2153)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2393..2412)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2740..2761
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5197..5785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5250..5270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5668..5679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5872..6105
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6177..6298
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6214..6233)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6268..6287
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6338..6473
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6505..6521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6505..6521)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6518..6540)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6529..6545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6553..6559,6560..6577,6578..6583))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6598..6619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6736..6753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6907..7495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6987..7006)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7666..8457,8458..8526))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8289..8308
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8527..8631)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8699..8717
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8755..8777)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8877..8896
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9090..9112
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9104..9121
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9105..9121
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        9136..9362
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9363..9543
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
       61 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
      121 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
      181 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
      241 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
      301 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
      361 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
      421 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
      481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
      541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
      601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
      661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
      721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
      781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
      841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
      901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
      961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     1021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     1081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     1141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     1201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     1261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     1321 gaacggatct cgacggtatc ggttaacttt taaaagaaaa ggggggattg gggggtacag
     1381 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     1441 acaaattaca aaattcaaaa ttttatcgat aagcttggga gttccgcgtt acataactta
     1501 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     1561 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     1621 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     1681 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     1741 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     1801 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     1861 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     1921 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     1981 atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt
     2041 ttgacctcca tagaagacac cgactctagc gctaccggtc gccaccatgg tgagcaaggg
     2101 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     2161 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     2221 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt
     2281 cggctacggc ctgaagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     2341 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     2401 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     2461 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     2521 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2581 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2641 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     2701 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2761 gaccgccgcc gggatcactc acggcatgga cgagctgtac aagtactcag atctcgacat
     2821 ggagccccgc cccggagcga gtacccgccg gcctgagggc cgcccccagc gcgagcccgc
     2881 cccggatgtc tgggtgtttc cctgcgaccg agacctgccg gacagcagcg actctgaggc
     2941 ggagaccgaa gtgggggggc ggggggacgc cgaccaccat gacgacgact ccgcctccga
     3001 ggcggacagc acggacacgg aactgttcga gacggggctg ctggggccgc agggcgtgga
     3061 tgggggggcg gtctcggggg ggagcccccc ccgcgaggaa gaccccggca gttgcggggg
     3121 cgccccccct cgagaggacg gggggagcga cgagggcgac gtgtgcgccg tgtgcacgga
     3181 tgagatcgcg ccccacctgc gctgcgacac cttcccgtgc atgcaccgct tctgcatccc
     3241 gtgcatgaaa acctggatgc aattgcgcaa cacctgcccg ctgtgcaacg ccaagctggt
     3301 gtacctgata gtgggcgtga cgcccagcgg gtcgttcagc accatcccga tcgtgaacga
     3361 cccccagacc cgcatggagg ccgaggaggc cgtcagggcg ggcacggccg tggactttat
     3421 ctggacgggc aatcagcggt tcgccccgcg gtacctgacc ctgggggggc acacggtgag
     3481 ggccctgtcg cccacccacc cggagcccac cacggacgag gatgacgacg acctggacga
     3541 cgcagactac gtaccgcccg ccccccgccg gacgccccgc gcccccccac gcagaggcgc
     3601 cgccgcgccc cccgtgacgg gcggggcgtc tcacgcagcc ccccagccgg ccgcggctcg
     3661 gacagcgccc ccctcggcgc ccatcgggcc acacggcagc agtaacacca acaccaccac
     3721 caacagcagc ggcggcggcg gctcccgcca gtcgcgagcc gcggcgccgc ggggggcgtc
     3781 tggcccctcc gggggggttg gggttggggt tggggttgtt gaagcggagg cggggcggcc
     3841 gaggggccgg acgggccccc ttgtcaacag acccgccccc cttgcaaaca acagagaccc
     3901 catagtgatc agcgactccc ccccggcctc tccccacagg ccccccgcgg cgcccatgcc
     3961 aggctccgcc ccccgccccg ggccccccgc gtccgcggcc gcgtcgggac ccgcgcgccc
     4021 ccgcgcggcc gtggccccgt gcgtgcgagc gccgcctccg gggcccggcc cccgcgcccc
     4081 ggcccccggg gcggagccgg ccgcccgccc cgcggacgcg cgccgtgtgc cccagtcgca
     4141 ctcgtccctg gctcaggccg cgaaccaaga acagagtctg tgccgggcgc gtgcgacggt
     4201 ggcgcgcggc tcgggggggc cgggcgtgga gggtgggcac gggccctccc gcggcgccgc
     4261 cccctccggc gccgccccgc tcccctccgc cgcctctgtc gagcaggagg cggcggtgcg
     4321 tccgaggaag aggcgcgggt cgggccagga aaacccctcc ccccagtcca cgcgtccccc
     4381 cctcgcgccg gcaggggcca agagggcggc gacgcacccc ccctccgact cagggccggg
     4441 ggggcgcggc cagggtgggc ccgggacccc cctgacgtcc tcggcggcct ccgcctcttc
     4501 ctcctctgcc tcttcctcct cggccccgac ccccgcgggg gccgcctctt ccgccgccgg
     4561 ggccgcgtcc tcctccgctt ccgcctcctc gggcggggcc gtcggtgccc tgggagggag
     4621 acaagaggaa acctccctcg gcccccgcgc tgcttctggg ccgcgggggc cgaggaagtg
     4681 tgcccggaag acgcgccacg cggagacttc cggggccgtc cccgcgggcg gcctcacgcg
     4741 ctacctgccc atctcggggg tctctagcgt ggtcgccctg tcgccttacg tgaacaagac
     4801 tatcacgggg gactgcctgc ccatcctgga catggagacg gggaacatcg gggcgtacgt
     4861 ggtcctggtg gaccagacgg gaaacatggc gacccggctg cgggccgcgg tccccggctg
     4921 gagccgccgc accctgctcc ccgagaccgc gggtaaccac gtgatgcccc ccgagtaccc
     4981 gacggccccc gcgtcggagt ggaacagcct ctggatgacc cccgtgggga acatgctgtt
     5041 cgaccagggc accctagtgg gcgccctgga cttccgcagc ctgcggtctc ggcacccgtg
     5101 gtccggggag cagggggcgt cgacccggga cgagggaaaa caataagaat tctgcagtcg
     5161 acggtaccgc gggcccggga tccgcgacgc gtcgacaatc aacctctgga ttacaaaatt
     5221 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5281 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5341 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5401 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5461 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5521 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5581 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5641 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5701 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5761 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     5821 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     5881 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     5941 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     6001 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     6061 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     6121 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     6181 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6241 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6301 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6361 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6421 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6481 ttgcgggccc aaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
     6541 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6601 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6661 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6721 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6781 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6841 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     6901 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     6961 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7021 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7081 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7141 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7201 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7261 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7321 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7381 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7441 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7501 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7561 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7621 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7681 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7741 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7801 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7861 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     7921 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     7981 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8041 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8101 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8161 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8221 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8281 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8341 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8401 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8461 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8521 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8581 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8641 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8701 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     8761 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8821 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     8881 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     8941 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     9001 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9061 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     9121 tgccaagctg acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     9181 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     9241 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     9301 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     9361 acgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     9421 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     9481 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9541 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggag
//
