LOCUS       sequence-266029-        1526 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Visualization of the At1aR DRY mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Pietraszewska-Bogiel A, Joosen L, Chertkova AO, Goedhart J
  TITLE     Not So Dry After All: DRY Mutants of the AT1A Receptor and H1 
            Receptor Can Induce G-Protein-Dependent Signaling.
  JOURNAL   ACS Omega. 2020 Feb 3;5(6):2648-2659. doi: 10.1021/acsomega.9b03146.
            eCollection 2020 Feb 18.
  PUBMED    32095688
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.9b03146"; journalName: "ACS Omega"; date: 
            "2020-02-3- 3"; volume: "5"; issue: "6"; pages: "2648-2659"
COMMENT     Sequence Label: RnAT1aR DRY->AAY-mCherry
FEATURES             Location/Qualifiers
     source          1..1526
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..731)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(299..318)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     568..586
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      728..737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggggacgagt cgcggccgct ttacttgtac agctcgtcca tgccgccggt ggagtggcgg
       61 ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc
      121 aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc cttgtaggtg
      181 gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg cttgatctcg
      241 cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca gcccatggtc
      301 ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt caccttgtag
      361 atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc gccgtcctcg
      421 aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa gtagtcgggg
      481 atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg ggacaggatg
      541 tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc
      601 tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc
      661 tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc
      721 ttgctcacca tggtggcgac cggttccacc tcaaaacaag acgcaggctt tttggccgat
      781 gagctcatgt tatccgaagg ccggtaagaa agcgtgctca ttttcgtaga caggcttgag
      841 tgggacttgg cctttggggg aatatatttc aggagctgga ggaaatactt tttaaatttc
      901 ttccccagaa agccgtagaa cagagggttc aggcagttgt taaaatacgc tatgcagatg
      961 gtgatgggca tggcagtgtc cacgatgtca gaaattttac agtcatggat gacgcccagc
     1021 tgaatcagca catccaggaa agtgaatatt tggtggggga cccaggaaaa gaagaagaaa
     1081 agcacaatcg ccataattat cctaaagatg tcatcgtttc ttggtttgtt cttttgaatt
     1141 tcataagcct tctttagagc tttccaaata agggtatagc tggtgaaaat gataaggaaa
     1201 gggaacaaaa gcccagaatt tcttggtaag gccagccctt ggggagcgtc aattccagat
     1261 caaatgaaac cgcacctggg aattggggtt cccaagaaga taactttccg gggataacct
     1321 gcaaatggcc acccgccttc cccaaaagaa gcggggattt tgcccccctt tggggggaag
     1381 ggaaattttt gggggaaggc cggaggggga ttgaaaacaa aaaaaaaccg ggtgaaagga
     1441 acccctcccc aaactttttt tttttggggg gcctctcttt ttatccccgg cgggaaaaaa
     1501 caaccagaaa aaaaaagacg cccttt
//
