LOCUS       sequence-26603-e         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Funakoshi M, Hochstrasser M
  TITLE     Small epitope-linker modules for PCR-based C-terminal tagging in 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2009 Feb 25. 26(3):185-192.
  PUBMED    19243080
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2009
            Feb 25. 26(3):185-192."
COMMENT     Sequence Label: pFA6a-6xGLY-FLAG-HIS3MX6
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(81..278)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(572..586)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     gap             605..639
                     /estimated_length=35
ORIGIN
        1 taggcnncta gtgganctga tatcatcgat gaattcgagc tcgtttaaac tggatggcgg
       61 cgttagtatc gaatcgacag cagtatagcg accagcattc acatacgatt gacgcatgat
      121 attactttct gcgcacttaa cttcgcatct gggcagatga tgtcgaggcg aaaaaaaata
      181 taaatcacgc taacatttga ttaaaataga acaactacaa tataaaaaaa ctatacaaat
      241 gacaagttct tgaaaacaag aatcttttta ttgtcagtac ttcacatcaa aacacctttg
      301 gttgagggaa cgtcattggt gccattgcta gaaatagctt ctcttatggc aacagccaaa
      361 gccttgaacg cactctcact tctgtggtga tcgttgaaac ctctcagaca atcaacatgc
      421 aaagtaattc tggccgcctc cgcgaaactt tccaaaaagt gtggaatcat ttcagtggat
      481 aaatcaccaa tcatctctct cttcaatcca aggtcgatta cagcaaatgg tctactagat
      541 aaatcgacta cggcacgtga tagcgcctca tccaatggtg cgaacccagt accgaatctt
      601 tttannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc tngcnnnnan tgcnnatacc
      661 ngcaatcttc ggtagtatgg tgatcgtcaa tgtgcaggtc accaatacat tcaacaataa
      721 gagaccaacc agagtgtttt gccaacgcat ggatcatatg atccaaaaag ccaacacctg
      781 tgtgaatatc gatga
//
