LOCUS       sequence-266061-        5405 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Visualization of beta-arrestin2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Pietraszewska-Bogiel A, Joosen L, Chertkova AO, Goedhart J
  TITLE     Not So Dry After All: DRY Mutants of the AT1A Receptor and H1 
            Receptor Can Induce G-Protein-Dependent Signaling.
  JOURNAL   ACS Omega. 2020 Feb 3;5(6):2648-2659. doi: 10.1021/acsomega.9b03146.
            eCollection 2020 Feb 18.
  PUBMED    32095688
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.9b03146"; journalName: "ACS Omega"; date: 
            "2020-02-3- 3"; volume: "5"; issue: "6"; pages: "2648-2659"
COMMENT     Sequence Label: mCherry-RnBarr2
FEATURES             Location/Qualifiers
     source          1..5405
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(124..142)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    1953..2009
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    2133..2254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2170..2189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2224..2243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(2261..2716)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2398..2419)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2610..2629
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2743..2847
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2849..3206
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        2849..3040
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(2872..2892)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      3057..3192
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3241..4035
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3241..4035
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3295..3314)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3905..3924
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(4223..4242)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4267..4314
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4643..5231
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5132..5151
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
       61 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      121 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      181 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      241 tgaagctgtc cttccccgag ggcttcaatt gggagcgcgt gatgaacttc gaggacggcg
      301 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      361 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagtgtcgt accatgggct
      421 gggaggcctc cactgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      481 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      541 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcga catcaagttg gacatccttt
      601 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      661 ccggcggcat ggacgagctg tacaagtcca tgggcgacaa agggacacga gtgttcaaga
      721 aggcaagccc caatggaaag ctcaccgtct acctgggaaa gcgggacttt gtggaccaca
      781 ttgacctggt ggaccccgtg gatggcgtgg tcctggtgga tcctgagtat ctcaaagaaa
      841 ggcgagtcta cgtgacactg acctgcgcct tccggtatgg ccgggaagac ctggatgtct
      901 tgggtctgac ttttcgcaaa gacctgtttg tggctaacgt gcagtccttc ccaccggccc
      961 ctgaggacaa gaagccactg actcggctac aagagcgact catcaagaag ctgggcgagc
     1021 atgcctaccc cttcaccttt gagatcccgc caaaccttcc gtgctcagtc acattgcaac
     1081 ctgggcctga ggacacaggg aaggcctgcg gtgtggatta tgaagtgaaa gccttctgtg
     1141 ctgagaacct ggaggagaag atccacaaaa ggaattctgt gcggctagtc atccggaagg
     1201 ttcaatatgc ccctgagagg cctggccctc agcccacggc tgagaccacc agacagttcc
     1261 tcatgtcgga caagcccctg caccttgagg catctctgga taaggagatc tattatcatg
     1321 gagaacccat cagcgtcaat gtccatgtca ccaacaacac caacaagact gtgaagaaga
     1381 tcaagatctc ggtgcgccag tatgcagaca tctgtctctt caacacagct cagtacaagt
     1441 gcccagtggc catggaggaa gctgatgata ctgtggcacc cagctcaaca ttctgcaagg
     1501 tctacacact gactcccttc ctggcaaaca acagagagaa gcgggggctt gccctcgacg
     1561 ggaagctcaa gcatgaagac acaaatctgg cttccagcac tctgttgcgg gaaggcgcca
     1621 accgtgaaat cctgggtatc attgtttcct acaaagtcaa agtgaagctg gtggtgtccc
     1681 ggggcggcct gttgggagac cttgcatcca gtgatgtggc tgtggaactg ccctttacct
     1741 taatgcaccc caagcctaaa gaggagcccc cacatcggga agttccagag agcgagactc
     1801 cagtagacac caatctcata gagcttgaca ccaatgatga cgacattgtg tttgaggact
     1861 ttgctcgtca gcggctgaaa ggcatgaagg atgacaagga cgaagaggat gatggcaccg
     1921 gctctccaca cctcaacaac agataaggac tcagatctcg agctcaagct tcgaattctg
     1981 cagtcgacgg taccgcgggc ccgggatcca ccggatctag ataactgatc ataatcagcc
     2041 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2101 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2161 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2221 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgttaatatt
     2281 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     2341 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     2401 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     2461 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     2521 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     2581 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     2641 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     2701 ccgctacagg gcgcgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     2761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     2821 cttcaataat attgaaaaag gaagagtcct gaggcggaaa gaaccagctg tggaatgtgt
     2881 gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     2941 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     3001 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     3061 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     3121 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     3181 tttttggagg cctaggcttt tgcaaagatc gatcaagaga caggatgagg atcgtttcgc
     3241 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     3301 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     3361 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     3421 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     3481 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     3541 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     3601 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     3661 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     3721 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     3781 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     3841 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     3901 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     3961 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     4021 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     4081 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     4141 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     4201 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     4261 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     4321 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     4381 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     4441 ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc ctcaggttac tcatatatac
     4501 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     4561 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     4621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     4681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     4741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     4801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     4861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     4921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     4981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5221 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     5281 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcca
     5341 tgcattagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     5401 ttccg
//
