LOCUS       sequence-266073-        7045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses YFP-ICP0 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7045)
  AUTHORS   Newhart A, Rafalska-Metcalf IU, Yang T, Negorev DG, Janicki SM
  TITLE     Single-cell analysis of Daxx and ATRX-dependent transcriptional 
            repression.
  JOURNAL   J Cell Sci. 2012 Nov 15;125(Pt 22):5489-501. doi: 
            10.1242/jcs.110148. Epub 2012 Sep 12.
  PUBMED    22976303
REFERENCE   2  (bases 1 to 7045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-11-15"; volume: "125(Pt 22):5489-501. doi"; 
            pages: " 10.1242/jcs.110148. Epub 2012 Sep 12"
COMMENT     Sequence Label: YFP-ICP0-C3
FEATURES             Location/Qualifiers
     source          1..7045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced YFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3833..3954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3870..3889)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3924..3943
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3961..4416)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4098..4119)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4310..4329
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4443..4547
                     /gene="bla"
                     /label=AmpR promoter
     promoter        4549..4906
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        4549..4740
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(4572..4592)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      4757..4892
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4941..5735
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4941..5735
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4995..5014)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5605..5624
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(5923..5942)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5967..6014
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6343..6931
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6832..6851
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccttcggc tacggcctga agtgcttcgc ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag ctaccagtcc aagctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactcacgg catggacgag
     1321 ctgtacaagt actcagatct cgacatggag ccccgccccg gagcgagtac ccgccggcct
     1381 gagggccgcc cccagcgcga gcccgccccg gatgtctggg tgtttccctg cgaccgagac
     1441 ctgccggaca gcagcgactc tgaggcggag accgaagtgg gggggcgggg ggacgccgac
     1501 caccatgacg acgactccgc ctccgaggcg gacagcacgg acacggaact gttcgagacg
     1561 gggctgctgg ggccgcaggg cgtggatggg ggggcggtct cgggggggag ccccccccgc
     1621 gaggaagacc ccggcagttg cgggggcgcc ccccctcgag aggacggggg gagcgacgag
     1681 ggcgacgtgt gcgccgtgtg cacggatgag atcgcgcccc acctgcgctg cgacaccttc
     1741 ccgtgcatgc accgcttctg catcccgtgc atgaaaacct ggatgcaatt gcgcaacacc
     1801 tgcccgctgt gcaacgccaa gctggtgtac ctgatagtgg gcgtgacgcc cagcgggtcg
     1861 ttcagcacca tcccgatcgt gaacgacccc cagacccgca tggaggccga ggaggccgtc
     1921 agggcgggca cggccgtgga ctttatctgg acgggcaatc agcggttcgc cccgcggtac
     1981 ctgaccctgg gggggcacac ggtgagggcc ctgtcgccca cccacccgga gcccaccacg
     2041 gacgaggatg acgacgacct ggacgacgca gactacgtac cgcccgcccc ccgccggacg
     2101 ccccgcgccc ccccacgcag aggcgccgcc gcgccccccg tgacgggcgg ggcgtctcac
     2161 gcagcccccc agccggccgc ggctcggaca gcgcccccct cggcgcccat cgggccacac
     2221 ggcagcagta acaccaacmc cmccaccaac agcagcggcg gcggcggcyc ccgccagtcg
     2281 cgagccgcgg cgccgcgggg ggcgtctggc ccctccgggg gggttggggt tggggttggg
     2341 gttgttgaag cggaggcggg gcggccgagg ggccggacgg gcccccttgt caacagaccc
     2401 gccccccttg caaacaacag agaccccata gtgatcagcg actccccccc ggcctctccc
     2461 cacaggcccc ccgcggcgcc catgccaggc tccgcccccc gccccgggcc ccccgcgtcc
     2521 gcggccgcgt cgggacccgc gcgcccccgc gcggccgtgg ccccgtgcgt gcgagcgccg
     2581 cctccggggc ccggcccccg cgccccggcc cccggggcgg agccggccgc ccgccccgcg
     2641 gacgcgcgcc gtgtgcccca gtcgcactcg tccctggctc aggccgcgaa ccaagaacag
     2701 agtctgtgcc gggcgcgtgc gacggtggcg cgcggctcgg gggggccggg cgtggagggt
     2761 gggcacgggc cctcccgcgg cgccgccccc tccggcgccg ccccgctccc ctccgccgcc
     2821 tctgtcgagc aggaggcggc ggtgcgtccg aggaagaggc gcgggtcggg ccaggaaaac
     2881 ccctcccccc agtccacgcg tccccccctc gcgccggcag gggccaagag ggcggcgacg
     2941 caccccccct ccgactcagg gccggggggg cgcggccagg gtgggcccgg gacccccctg
     3001 acgtcctcgg cggcctccgc ctcttcctcc tctgcctctt cctcctcggc cccgaccccc
     3061 gcgggggccg cctcttccgc cgccggggcc gcgtcctcct ccgcttccgc ctcctcgggc
     3121 ggggccgtcg gtgccctggg agggagacaa gaggaaacct ccctcggccc ccgcgctgct
     3181 tctgggccgc gggggccgag gaagtgtgcc cggaagacgc gccacgcgga gacttccggg
     3241 gccgtccccg cgggcggcct cacgcgctac ctgcccatct cgggggtctc tagcgtggtc
     3301 gccctgtcgc cttacgtgaa caagactatc acgggggact gcctgcccat cctggacatg
     3361 gagacgggga acatcggggc gtacgtggtc ctggtggacc agacgggaaa catggcgacc
     3421 cggctgcggg ccgcggtccc cggctggagc cgccgcaccc tgctccccga gaccgcgggt
     3481 aaccacgtga tgccccccga gtacccgacg gcccccgcgt cggagtggaa cagcctctgg
     3541 atgacccccg tggggaacat gctgttcgac cagggcaccc tagtgggcgc cctggacttc
     3601 cgcagcctgc ggtctcggca cccgtggtcc ggggagcagg gggcgtcgac ccgggacgag
     3661 ggaaaacaat aagaattctg cagtcgacgg taccgcgggc ccgggatcca ccggatctag
     3721 ataactgatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3781 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3841 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3901 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgt
     3961 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     4021 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     4081 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     4141 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4201 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4261 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4321 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4381 cacccgccgc gcttaatgcg ccgctacagg gcgcgtcagg tggcactttt cggggaaatg
     4441 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4501 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtcct gaggcggaaa
     4561 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     4621 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     4681 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     4741 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     4801 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     4861 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaagatc gatcaagaga
     4921 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
     4981 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     5041 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     5101 ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg gccacgacgg
     5161 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     5221 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     5281 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     5341 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     5401 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     5461 tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     5521 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     5581 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     5641 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     5701 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     5761 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     5821 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     5881 tctcatgctg gagttcttcg cccaccctag ggggaggcta actgaaacac ggaaggagac
     5941 aataccggaa ggaacccgcg ctatgacggc aataaaaaga cagaataaaa cgcacggtgt
     6001 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     6061 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     6121 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     6181 ctcaggttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     6241 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     6301 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6361 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6421 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6481 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6541 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6601 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6661 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6721 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6781 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6841 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     6901 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     6961 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7021 ggataaccgt attaccgcca tgcat
//
