LOCUS       sequence-266089-        7132 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7132)
  TITLE     Second Batch
REFERENCE   2  (bases 1 to 7132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGL3-Basic-Pax8
FEATURES             Location/Qualifiers
     source          1..7132
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    117..165
                     /note="synthetic polyadenylation signal"
     misc_feature    179..270
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     219..238
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     regulatory      2673..2682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2679..4331
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2745..2763)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    4372..4493
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4383..4402)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4437..4456
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4741..4758)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4912..5500)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4992..5011)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5671..6462,6463..6531))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6294..6313
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6532..6636)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6663..7118
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6750..6769)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6960..6981
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagccc
       61 aagctaccat gataagtaag taatattaag gtacgggagg tacttggagc ggccgcaata
      121 aaatatcttt attttcatta catctgtgtg ttggtttttt gtgtgaatcg atagtactaa
      181 catacgctct ccatcaaaac aaaacgaaac aaaacaaact agcaaaatag gctgtcccca
      241 gtgcaagtgc aggtgccaga acatttctct atcgataggt accgagctct tacgcgtgct
      301 agcccgggct cgagatatca gattcaccct ggagcatcgc ctccctggtg ggagggaatc
      361 ctgggtggac aaagagagtt tctgctgtgg ctgccacact ccacacctcc cctgcagcag
      421 gacacaatcc agggcagcaa actggcccat ggggtccgga ggacctatgc caagatccat
      481 ttccatccca acacccttgg attgtctctc tctgggaggg actgccagag aagttaggga
      541 tctggagctt tgaaaaccac agagatattt aaaattacaa ttttagagaa agatttaaaa
      601 tgatgaagca ccaactattt ttgtttacag cccctctttg caaatgtgga ggaaacatgt
      661 agggatacac atcttcttct ctcttttttc acaagatgat tcatttctcc taaattgaga
      721 aatgagaaag actgggtgga aggaggttgt ggattgacat gaaagagaaa acttttttaa
      781 ttcagaaaaa tagttcatgt gttttcacac acgtgtacat atgtgcatac acacacacac
      841 acacacacac acacacacat ccaatccccc tgaatatccc agtttcccct gcaccttcct
      901 tgcccccctc ccactggctt cttagcctcc tgtcccagaa caaccccacc aggcctgcag
      961 agccatcact gaaaagggca tggctgtcct ctagactgta atcctgagtc cagaggtctg
     1021 gccaaagatt ggaggagcat ccaggccttg acccccaggc ctgccagtgt tccaggacat
     1081 ttgctcactc atacccactc acaatcttac gtgtaccccg gttccccgac tgagagaact
     1141 atgaggaagg agggggcagc ttttgccatc actaaggtaa agaggtgcca ggcagctctg
     1201 ctcggtccca gggctcacca accctgccat cattcccacc agtggacccc ttcttaacca
     1261 cacccaggcc cccatattgc tgaggctatt gattcctaca gaccaggcca gaggtcagct
     1321 cagtcctgtt cctttctaag gcctttccac acagagatga agtctaggcc ctcaatgccc
     1381 ttcccctgaa ctcttcttcc aagttcaaaa tcctcaagga gcaagaaaga gggcaggtgg
     1441 ctgcgtgtgt gttccagagg agagaggcga ggagttgctg tggaatgggt tatgtgacaa
     1501 ttttgttagc ctgacaatgc aagggtgtga acgctatctg tgtagaacag agggaatggc
     1561 ttcaggtgct gctcaggatt cttccctgga agaggtcata gctgtggtgg gaaggtaata
     1621 gcatcttgcc tacagccccg aaagcaggct ctacctaaag aatcaggcat aaaacagaag
     1681 ttccctcctt ccttttacaa aagaaaaggc tgcttcctgg tgagcaaacc aggggagaca
     1741 gggggcaagg agggcatcct acagagacca tccctctcac ttctggtggt cagtgaccca
     1801 ggaggaccca gagaacctca tcctcaggct cctaggcctc tgttccccac tcctccaata
     1861 tttccaggcc tgctgagtta gggctcagct gcctccctct tccctccgct ggaaacagaa
     1921 gctcccagcg aatgtttcta actcctgagt ccactcagcc atgtccccct tgcaaaatcc
     1981 tccccttccc gcacccccct gcacccagcc agccaatccg cggcctacga gcctcgccag
     2041 acccatctcc cagggcctca gcgggataaa accggtcggt gatgccgggt ggatgggaac
     2101 aaacttcaga aggaggagag acaccgggcc cagggcaccc tcgcgggcgg acccaagcag
     2161 tgagggcctg cagccggccg gccaggtatg tcacccaggg gttagctgga agctggctag
     2221 cagtgaggac gggggatgga agaaaggaga gggtcccagg atgcctggca gccttttccc
     2281 tcccaagtta aacgggataa gactgggaca gcggagggag tgggcacgaa ggttggagtc
     2341 tggagcttct tcagcgcact cccaatcctt gatcctcccg ggaagcctgt tagctaggct
     2401 aggctgaggt tggccctact tcgcctaaaa atcctcctac tcctggcaga cgatgcaggt
     2461 gtaaaggatg aggcctgggg agggggcctg aggatgcagg catcgaatct catcgcatct
     2521 catgcccttc tcctgggttt gtgcagggca gcggcaggcg cggcccggac ctacgggagg
     2581 aagccccgag ccctcggcgg gctgcgagcg actccccggc gatgcctcac aactccatca
     2641 gataagcttg gcattccggt actgttggta aagccaccat ggaagacgcc aaaaacataa
     2701 agaaaggccc ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata
     2761 aggctatgaa gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg
     2821 aggtggacat cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga
     2881 aacgatatgg gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat
     2941 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca
     3001 tttataatga acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg
     3061 tttccaaaaa ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa
     3121 aaattattat catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg
     3181 tcacatctca tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata
     3241 gggacaagac aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg
     3301 tcgctctgcc tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg
     3361 gcaatcaaat cattccggat actgcgattt taagtgttgt tccattccat cacggttttg
     3421 gaatgtttac tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat
     3481 ttgaagaaga gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg
     3541 tgccaaccct attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta
     3601 atttacacga aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg
     3661 ccaagaggtt ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag
     3721 ctattctgat tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat
     3781 tttttgaagc gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag
     3841 gcgaactgtg tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga
     3901 ccaacgcctt gattgacaag gatggatggc tacattctgg agacatagct tactgggacg
     3961 aagacgaaca cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc
     4021 aggtggctcc cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag
     4081 gtgtcgcagg tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg
     4141 agcacggaaa gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa
     4201 ccgcgaaaaa gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg
     4261 gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga
     4321 tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg ataagataca
     4381 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     4441 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     4501 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     4561 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat gcccttgaga
     4621 gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt
     4681 atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct cttccgcttc
     4741 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4801 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4861 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4921 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4981 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     5041 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     5101 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     5161 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     5221 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     5281 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     5341 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5401 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5461 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5521 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5581 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5641 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5701 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5761 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5821 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5881 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5941 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     6001 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     6061 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     6121 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     6181 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     6241 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     6301 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6361 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6421 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6481 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6541 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6601 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6661 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6721 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6781 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6841 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     6901 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     6961 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7021 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7081 taacgcgaat tttaacaaaa tattaacgct tacaatttgc cattcgccat tc
//
